##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630317.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	312042
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.103986001884362	31.0	28.0	31.0	25.0	34.0
2	29.46848501163305	31.0	28.0	31.0	25.0	34.0
3	29.54758654283718	31.0	30.0	31.0	25.0	34.0
4	33.08023919856942	35.0	32.0	37.0	27.0	37.0
5	31.39870273873389	35.0	30.0	35.0	19.0	37.0
6	31.83236231020183	35.0	30.0	35.0	23.0	37.0
7	32.02014151941085	35.0	31.0	35.0	25.0	37.0
8	31.94806147890348	35.0	31.0	35.0	23.0	37.0
9	33.24406650386807	35.0	32.0	38.0	25.0	39.0
10	32.641551457816576	35.0	30.0	37.0	23.0	39.0
11	33.114930041468774	35.0	32.0	38.0	25.0	39.0
12	32.762714634568425	35.0	30.0	37.0	23.0	39.0
13	33.095775568673446	35.0	32.0	38.0	25.0	39.0
14	33.56282167144167	36.0	31.0	39.0	24.0	40.0
15	33.554217060523904	36.0	31.0	39.0	24.0	40.0
16	33.4947987770877	36.0	31.0	39.0	24.0	40.0
17	33.36546362348658	36.0	31.0	38.0	24.0	40.0
18	33.27191211439486	36.0	31.0	38.0	24.0	40.0
19	33.294364220201125	36.0	31.0	39.0	23.0	40.0
20	33.174681613372556	36.0	31.0	39.0	22.0	40.0
21	33.14025996500471	36.0	30.0	39.0	22.0	40.0
22	33.07809525640779	36.0	30.0	39.0	22.0	40.0
23	32.923093686106355	36.0	30.0	39.0	21.0	40.0
24	32.86182949731126	36.0	30.0	39.0	21.0	40.0
25	32.808019433281416	36.0	30.0	39.0	20.0	40.0
26	32.48724210202473	35.0	30.0	38.0	19.0	40.0
27	32.34710391549856	35.0	30.0	38.0	18.0	40.0
28	32.103559777209476	35.0	29.0	38.0	18.0	40.0
29	31.945465674492535	35.0	29.0	38.0	18.0	40.0
30	31.785211606129945	35.0	29.0	38.0	17.0	40.0
31	31.56633401913845	34.0	28.0	38.0	16.0	40.0
32	31.367001877952326	34.0	27.0	38.0	16.0	40.0
33	31.241156639170367	34.0	27.0	38.0	16.0	40.0
34	31.05109248114036	34.0	27.0	38.0	16.0	40.0
35	30.931525243396724	34.0	27.0	38.0	15.0	40.0
36	30.745255446382217	34.0	26.0	38.0	15.0	40.0
37	30.579338678767602	34.0	26.0	38.0	15.0	40.0
38	30.457448035841328	34.0	26.0	38.0	15.0	40.0
39	30.240403535421514	33.0	25.0	38.0	14.0	40.0
40	30.053938251901986	33.0	25.0	38.0	13.0	40.0
41	29.819328808301446	33.0	25.0	38.0	12.0	40.0
42	29.69080444299165	33.0	24.0	38.0	11.0	40.0
43	28.558203703347626	32.0	23.0	37.0	9.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	6.0
11	13.0
12	34.0
13	69.0
14	119.0
15	232.0
16	463.0
17	767.0
18	1144.0
19	1834.0
20	2605.0
21	3780.0
22	4965.0
23	6675.0
24	8388.0
25	10166.0
26	12504.0
27	14651.0
28	16669.0
29	18984.0
30	20641.0
31	21790.0
32	22791.0
33	23631.0
34	23587.0
35	23142.0
36	22488.0
37	20893.0
38	19021.0
39	9987.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.7842021266368	24.446388627171984	14.653155664942542	22.116253581248678
2	21.368918286640902	25.116490728812146	31.218874382294693	22.29571660225226
3	23.23341088699598	24.74025932406535	29.218182167785105	22.808147621153562
4	18.89905846007909	18.124483242640412	32.86352478192038	30.112933515360112
5	14.970420648502444	38.40124085860237	32.38089744329289	14.247441049602298
6	33.24680651963517	37.59109350664333	14.977470981470443	14.184628992251044
7	29.38226264413124	31.971337191788287	20.68727927650765	17.959120887572826
8	27.635702886149943	35.30678562501202	19.22561706436954	17.8318944244685
9	27.0383474019523	14.117009889694335	20.13991706244672	38.70472564590664
10	17.38900532620609	28.654476000025635	32.840450964934206	21.116067708834066
11	36.81395453176175	22.519083969465647	20.655232308471298	20.011729190301306
12	21.05742175732754	26.934194755834152	29.700489036732236	22.307894450106076
13	31.115362675537266	20.335403567468482	25.44144698470078	23.107786772293473
14	22.910697918869896	21.591965184173926	26.166669871363467	29.33066702559271
15	25.072586382602342	28.874318200755027	22.980880778869512	23.07221463777312
16	25.132193743149962	27.058216522134842	24.780318034110792	23.029271700604408
17	23.58720941411733	27.616154235647766	25.830497176662114	22.966139173572788
18	22.98632876343569	25.554893251549473	28.217034886329408	23.241743098685433
19	24.896328058402396	26.34933758917069	27.776068606149174	20.978265746277742
20	24.741541202786806	25.485992270271307	27.44822812313727	22.324238403804618
21	24.620724133289748	25.753263983694502	26.94509072496651	22.680921158049237
22	25.28249402324046	25.130270925067776	27.660379051537937	21.926856000153823
23	23.330513200146132	25.70070695611488	28.278565064959203	22.69021477877978
24	23.94196935027977	26.013485364149698	27.719665942405193	22.324879343165343
25	24.068875343703734	25.55329090314765	27.766454515738264	22.611379237410347
26	24.319803103428384	26.17372020433147	27.158203062408266	22.348273629831883
27	24.482922170733428	25.584376462142917	26.64481063446587	23.287890732657786
28	24.16469577813243	25.567391569083647	28.267989565507207	21.999923087276713
29	24.61816037584684	25.939456867985722	28.180821812448325	21.261560943719115
30	24.42716044635017	25.79300222405958	27.093788656655192	22.68604867293505
31	23.80096269091981	25.37927586671025	28.1423654508047	22.677395991565238
32	23.58881176251915	25.90676896058864	27.830227982130612	22.674191294761602
33	24.10572935694554	25.401067804974968	27.84080348158261	22.652399356496883
34	24.786086488357338	25.33793527794335	27.69979682222265	22.176181411476662
35	24.937668647169293	25.53662647976875	27.222937937841703	22.30276693522026
36	23.915370366809597	25.706475410361424	26.721082418392395	23.657071804436583
37	23.82692073502926	25.51964158670948	28.21030502304177	22.44313265521949
38	23.96376128854449	24.920042814749298	27.896885675646228	23.219310221059985
39	24.267246075848764	24.976765948173643	28.284013049525385	22.47197492645221
40	24.535158728632684	25.03893706616417	28.073464469526538	22.352439735676608
41	23.692323469276573	25.324796021048453	28.05487722806545	22.928003281609527
42	23.837496234481257	25.994257183327885	27.492132469347077	22.676114112843784
43	24.40985508361054	24.455361778222162	27.690182731811742	23.444600406355555
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	217.0
1	164.5
2	112.0
3	169.5
4	227.0
5	227.0
6	391.5
7	556.0
8	522.0
9	488.0
10	656.0
11	824.0
12	824.0
13	1506.0
14	2188.0
15	3195.0
16	4202.0
17	3946.0
18	3690.0
19	3690.0
20	4178.0
21	4666.0
22	4240.0
23	3814.0
24	4307.5
25	4801.0
26	4801.0
27	5279.0
28	5757.0
29	6491.5
30	7226.0
31	8165.5
32	9105.0
33	9105.0
34	11305.5
35	13506.0
36	14554.5
37	15603.0
38	15904.0
39	16205.0
40	16205.0
41	16783.0
42	17361.0
43	18173.0
44	18985.0
45	19087.5
46	19190.0
47	19190.0
48	19465.5
49	19741.0
50	20195.5
51	20650.0
52	21622.0
53	22594.0
54	22594.0
55	22007.5
56	21421.0
57	19547.5
58	17674.0
59	16958.0
60	16242.0
61	16242.0
62	14883.0
63	13524.0
64	11410.5
65	9297.0
66	7949.5
67	6602.0
68	6602.0
69	5961.5
70	5321.0
71	4938.5
72	4556.0
73	3964.5
74	3373.0
75	3373.0
76	2204.0
77	1035.0
78	795.0
79	555.0
80	426.5
81	298.0
82	298.0
83	225.5
84	153.0
85	136.5
86	120.0
87	104.0
88	88.0
89	88.0
90	66.5
91	45.0
92	32.5
93	20.0
94	14.0
95	8.0
96	8.0
97	5.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	312042.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.68645210015137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.19337533974426	66.09015985243855
2	4.573145194992657	6.556651079357572
3	1.151813822174575	2.4770833917482986
4	0.5499255597099967	1.5768884917919845
5	0.3108829142303772	1.1143046569865704
6	0.2289788170626378	0.9848807400786067
7	0.14331414294900194	0.7191577710651512
8	0.1016118149801806	0.5827352405908928
9	0.09159384308152356	0.590943388025918
>10	0.5339737825184728	7.484198653093401
>50	0.07435376966921378	3.755192602421431
>100	0.04434351323645602	5.688498687002561
>500	0.0013437428253471522	0.686177562323576
>1k	0.0013437428253471522	1.6931278830754404
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2024	0.6486306330558066	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1934	0.6197883618230879	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1315	0.4214176296780562	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	893	0.28617942456464196	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	702	0.22496971561520562	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	542	0.17369456675703912	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	448	0.14357041680286628	No Hit
ATTTTATTTTGTATTTACAGTTTTCAGTTTCTAATGCAAGGGT	432	0.13844290191704964	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	408	0.13075162958832465	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	402	0.1288288115061434	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	401	0.12850834182577986	No Hit
ATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAAGAGAG	396	0.12690599342396217	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	364	0.11665096365232885	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	357	0.11440767588978408	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	338	0.1083187519628768	No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG	335	0.10735734292178617	No Hit
TTCTAATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAA	327	0.10479358547887785	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	322	0.10319123707706014	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	3.204696803635408E-4	0.0
19	0.0	0.0	0.0	3.204696803635408E-4	0.0
20	0.0	0.0	0.0	6.409393607270817E-4	0.0
21	0.0	0.0	0.0	9.614090410906224E-4	0.0
22	0.0	0.0	0.0	0.0025637574429083266	0.0
23	0.0	0.0	0.0	0.003204696803635408	0.0
24	0.0	0.0	0.0	0.00416610584472603	0.0
25	0.0	0.0	0.0	0.004807045205453112	0.0
26	0.0	0.0	0.0	0.007370802648361438	0.0
27	0.0	0.0	0.0	0.01249831753417809	0.0
28	0.0	0.0	0.0	0.03396978611853532	0.0
29	0.0	0.0	0.0	0.05928689086725505	0.0
30	0.0	0.0	0.0	0.09549996474833516	0.0
31	0.0	0.0	0.0	0.22240595817229733	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGTA	25	0.005490283	29.6	10
CGCGTAA	100	0.0	27.750002	10
GGTATCA	515	0.0	25.864077	1
GCGTAAC	115	0.0	25.73913	11
AGCGTCA	75	1.36551535E-8	24.666666	3
GTCGCGT	100	2.0008883E-11	24.050001	8
GGTCGCG	100	2.0008883E-11	24.050001	7
AAGTTGC	55	1.8969042E-5	23.545454	7
TAGGACA	40	0.0019280522	23.125	4
TCGCGTA	105	4.0017767E-11	22.904764	9
CGTAACT	130	0.0	22.76923	12
TTAATAC	50	2.6959923E-4	22.2	3
GTAACTA	125	0.0	22.199999	13
GGGTCGC	110	7.2759576E-11	21.863636	6
TCGTTCG	95	7.0940587E-9	21.421053	37
GCAGCGT	90	9.422911E-8	20.555555	1
AGTCTCG	135	1.8189894E-12	20.555553	33
TTGGATC	65	6.881553E-5	19.923077	34
GTCTCGT	130	3.274181E-11	19.923077	34
TCTCGTT	130	3.274181E-11	19.923077	35
>>END_MODULE