FastQCFastQC Report
Fri 10 Feb 2017
ERR1630316.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630316.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809700
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC44410.24539979002044537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24570.13576835939658505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22420.12388793722716473No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT19170.10592915952920375No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18800.10388462176051279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12450.018.722891
TTAGACT2350.017.3191494
AGACTAT1502.5102054E-1017.2666666
GTACCGT650.00158040817.0769236
GACAGGC11700.016.7606837
ATCGTAC904.4482284E-516.44444525
TTTAGCG803.383908E-416.187526
CGCGAAC1606.2937033E-1016.187528
TAGCGGG700.002593265415.85714228
GACGGTA1557.215931E-915.51612958
TGCATCA12350.015.42915114
CGTTATT1802.0190782E-1015.4166662
GTTCGCG1455.3505573E-815.31034630
CGCGCTT1701.4879333E-915.23529412
ATTAGAC1601.0975782E-815.0312513
TCGCGGA1601.0975782E-815.03125132
TATTAGA2850.014.9298242
AAGACGG3000.014.85
GACGGAC2900.014.6724157
GTATTAG5450.014.596331
Produced by FastQC (version 0.11.5)