##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630316.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1809700
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.043473503895676	33.0	31.0	34.0	30.0	34.0
2	32.24087141515168	33.0	31.0	34.0	30.0	34.0
3	32.389985080400066	34.0	31.0	34.0	30.0	34.0
4	36.005561695308614	37.0	35.0	37.0	35.0	37.0
5	35.924321710780795	37.0	35.0	37.0	35.0	37.0
6	35.98742830303365	37.0	35.0	37.0	35.0	37.0
7	35.969429739735865	37.0	35.0	37.0	35.0	37.0
8	35.952177709012545	37.0	35.0	37.0	35.0	37.0
9	37.69649002597116	39.0	37.0	39.0	35.0	39.0
10	37.60895673316019	39.0	37.0	39.0	35.0	39.0
11	37.696082223572965	39.0	37.0	39.0	35.0	39.0
12	37.575188705310275	39.0	37.0	39.0	35.0	39.0
13	37.61050063546444	39.0	37.0	39.0	35.0	39.0
14	38.89450792949108	40.0	38.0	41.0	36.0	41.0
15	38.89366138033928	40.0	38.0	41.0	36.0	41.0
16	38.88467867602365	40.0	38.0	41.0	36.0	41.0
17	38.85223130905675	40.0	38.0	41.0	35.0	41.0
18	38.808093606675136	40.0	38.0	41.0	35.0	41.0
19	38.81391777642703	40.0	38.0	41.0	35.0	41.0
20	38.762260043101065	40.0	38.0	41.0	35.0	41.0
21	38.731274796927664	40.0	38.0	41.0	35.0	41.0
22	38.701017295684366	40.0	38.0	41.0	35.0	41.0
23	38.654060341493064	40.0	38.0	41.0	34.0	41.0
24	38.65179919323645	40.0	38.0	41.0	34.0	41.0
25	38.623415483229266	40.0	38.0	41.0	34.0	41.0
26	38.49545836326463	40.0	38.0	41.0	34.0	41.0
27	38.40874012267226	40.0	38.0	41.0	34.0	41.0
28	38.34144388572692	40.0	38.0	41.0	34.0	41.0
29	38.28800906227551	40.0	38.0	41.0	34.0	41.0
30	38.23762336298834	40.0	38.0	41.0	34.0	41.0
31	38.16281096314306	40.0	38.0	41.0	34.0	41.0
32	38.08399292700447	40.0	38.0	41.0	33.0	41.0
33	38.02569431397469	40.0	38.0	41.0	33.0	41.0
34	37.940212189865726	40.0	37.0	41.0	33.0	41.0
35	37.85463004917942	40.0	37.0	41.0	33.0	41.0
36	37.77065480466376	40.0	37.0	41.0	33.0	41.0
37	37.7089296568492	40.0	37.0	41.0	33.0	41.0
38	37.61896281151572	40.0	37.0	41.0	33.0	41.0
39	37.54467480797922	40.0	37.0	41.0	32.0	41.0
40	37.449163397248164	40.0	37.0	41.0	32.0	41.0
41	37.339351273691776	39.0	36.0	41.0	32.0	41.0
42	37.29168370448141	39.0	36.0	41.0	32.0	41.0
43	36.17237166381168	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	2.0
10	6.0
11	1.0
12	1.0
13	2.0
14	1.0
15	3.0
16	11.0
17	26.0
18	40.0
19	81.0
20	183.0
21	386.0
22	671.0
23	1148.0
24	2007.0
25	3124.0
26	4821.0
27	7135.0
28	10112.0
29	14383.0
30	20479.0
31	27859.0
32	38045.0
33	51708.0
34	72817.0
35	102804.0
36	149319.0
37	233925.0
38	452415.0
39	616182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.27103939879538	17.829142951870473	11.576504392993314	27.323313256340832
2	18.76537547659833	19.475658948997072	34.298778803116534	27.460186771288058
3	19.6430900149196	21.584516770735483	29.548101895341773	29.22429131900315
4	14.491407415593747	14.845775542907663	34.47858761120628	36.18422943029231
5	15.106426479526993	36.00281814665414	33.93120406697243	14.95955130684644
6	35.92744653810024	34.59855224622866	15.247886390009393	14.226114825661712
7	31.098911421782617	29.823561916339724	20.287837763165165	18.789688898712495
8	28.768248881030008	33.15599270597336	18.868431231695862	19.20732718130077
9	27.469967397911255	13.628225672763442	18.318063767475273	40.58374316185003
10	17.29861302978394	26.12747969276676	32.288335083162956	24.285572194286345
11	37.655523014864336	20.760181245510307	20.67160302812621	20.912692711499144
12	22.68248881030005	24.195833563574073	28.57053655301984	24.551141073106038
13	30.96347460905122	18.35917555395922	24.106426479526995	26.57092335746256
14	23.008951759960215	19.326739238547823	24.56473448637896	33.099574515113
15	26.07835552854064	26.445046140244237	21.179974581422336	26.296623749792786
16	25.72647400121567	25.47963750897939	22.68226777918992	26.111620710615018
17	24.536829308725203	25.760789081063162	24.6769077747693	25.02547383544234
18	24.892855169365088	24.261258772172184	25.362159473945955	25.48372658451677
19	25.476598331215115	24.454716251312373	24.943526551362105	25.125158866110404
20	25.412665082610374	24.678399734762667	24.744764325578824	25.16417085704813
21	26.521854451013983	24.25208598110184	24.18743438138918	25.038625186495
22	26.102005857324418	24.36066751395259	24.635574957175223	24.90175167154777
23	25.431010664751064	24.300436536442504	24.614632259490524	25.653920539315912
24	25.44526717135437	24.580096148532906	24.532132397634967	25.442504282477756
25	25.79013096093275	24.096369564016136	24.852130187323866	25.26136928772725
26	25.879317013869702	24.624633917223846	24.649002597115544	24.847046471790904
27	25.770956512129082	24.53323755318561	24.30994087417804	25.385865060507268
28	25.084875946289444	24.249875670000552	25.22473338122341	25.440515002486602
29	24.873846493894018	24.55042272199812	25.375697629441348	25.200033154666517
30	24.822843565231807	25.068906448582638	24.953749240205557	25.154500745979995
31	25.320937171906944	24.63756423716638	24.59236337514505	25.44913521578162
32	24.686909432502624	24.607172459523678	25.0145880532685	25.691330054705197
33	24.939769022489916	24.46405481571531	25.11012875062165	25.486047411173125
34	25.753937116649166	24.425540144775375	24.602807095098637	25.21771564347682
35	25.59744709067801	24.412996629275572	25.343813891805272	24.645742388241146
36	25.342929767364758	24.81234458750069	24.19517047024369	25.649555174890864
37	25.33088357186274	24.06769077747693	25.13598939050671	25.465436260153616
38	25.253577941095205	24.227441012322483	25.413272918163234	25.105708128419074
39	24.56003757528872	24.389622589379456	25.58374316185003	25.466596673481796
40	24.846770182903242	23.763275681052107	26.40465270486821	24.98530143117644
41	24.00828866662983	23.867547107255348	26.55572746864121	25.568436757473616
42	24.366414322815935	24.213792341272033	26.06868541747251	25.35110791843952
43	24.314085207492955	22.94441067580262	26.720229872354533	26.02127424434989
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	87.0
1	81.5
2	76.0
3	221.0
4	366.0
5	366.0
6	449.0
7	532.0
8	545.0
9	558.0
10	723.0
11	888.0
12	888.0
13	1652.5
14	2417.0
15	3887.5
16	5358.0
17	5515.5
18	5673.0
19	5673.0
20	7761.5
21	9850.0
22	11072.5
23	12295.0
24	14636.0
25	16977.0
26	16977.0
27	19992.0
28	23007.0
29	27361.5
30	31716.0
31	38008.5
32	44301.0
33	44301.0
34	51226.5
35	58152.0
36	66396.0
37	74640.0
38	80195.5
39	85751.0
40	85751.0
41	92911.5
42	100072.0
43	104037.5
44	108003.0
45	111988.5
46	115974.0
47	115974.0
48	119676.5
49	123379.0
50	126501.5
51	129624.0
52	134404.0
53	139184.0
54	139184.0
55	137689.0
56	136194.0
57	128999.5
58	121805.0
59	114478.5
60	107152.0
61	107152.0
62	101333.5
63	95515.0
64	85208.0
65	74901.0
66	64958.5
67	55016.0
68	55016.0
69	47023.5
70	39031.0
71	33924.0
72	28817.0
73	26923.0
74	25029.0
75	25029.0
76	22199.5
77	19370.0
78	14955.5
79	10541.0
80	7211.5
81	3882.0
82	3882.0
83	2708.0
84	1534.0
85	1249.5
86	965.0
87	775.5
88	586.0
89	586.0
90	492.5
91	399.0
92	227.0
93	55.0
94	38.5
95	22.0
96	22.0
97	13.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1809700.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.664928719754776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.63371357724176	44.345323489713124
2	9.967401203571526	10.697997502217278
3	2.926219999710645	4.71106163108378
4	1.254142517767096	2.6921387528153997
5	0.6710471923068608	1.8005849871369635
6	0.43701120149401046	1.4071304986746276
7	0.30059099519381105	1.1291836031613216
8	0.2270339242375758	0.9747007488940571
9	0.17785408613432355	0.8590074169424039
>10	1.1485003962657887	12.091863765443417
>50	0.14501823076209167	5.4408873418758885
>100	0.10444227729481238	10.547473720487044
>500	0.00547489845659344	1.9236796300270778
>1k	0.0015494995631868229	1.378966911527724
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	4441	0.24539979002044537	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2457	0.13576835939658505	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2242	0.12388793722716473	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	1917	0.10592915952920375	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1880	0.10388462176051279	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	5.525777753218766E-5	0.0	0.0	0.0	0.0
7	5.525777753218766E-5	0.0	0.0	0.0	0.0
8	5.525777753218766E-5	0.0	0.0	0.0	0.0
9	5.525777753218766E-5	0.0	0.0	5.525777753218766E-5	0.0
10	5.525777753218766E-5	0.0	0.0	5.525777753218766E-5	0.0
11	5.525777753218766E-5	0.0	0.0	5.525777753218766E-5	0.0
12	5.525777753218766E-5	0.0	0.0	5.525777753218766E-5	0.0
13	5.525777753218766E-5	0.0	0.0	5.525777753218766E-5	0.0
14	5.525777753218766E-5	0.0	0.0	5.525777753218766E-5	0.0
15	5.525777753218766E-5	0.0	0.0	5.525777753218766E-5	0.0
16	5.525777753218766E-5	0.0	0.0	1.6577333259656298E-4	0.0
17	5.525777753218766E-5	0.0	0.0	2.7628888766093826E-4	0.0
18	5.525777753218766E-5	0.0	0.0	3.868044427253136E-4	0.0
19	5.525777753218766E-5	0.0	0.0	4.97319997789689E-4	0.0
20	5.525777753218766E-5	0.0	0.0	6.078355528540642E-4	0.0
21	1.1051555506437532E-4	0.0	0.0	8.841244405150025E-4	0.0
22	1.1051555506437532E-4	0.0	0.0	0.0015472177709012543	0.0
23	1.6577333259656298E-4	0.0	0.0	0.0024865999889484443	0.0
24	1.6577333259656298E-4	0.0	0.0	0.0033154666519312594	0.0
25	1.6577333259656298E-4	0.0	0.0	0.004310106647510637	0.0
26	1.6577333259656298E-4	0.0	0.0	0.005967839973476267	0.0
27	1.6577333259656298E-4	0.0	0.0	0.01464331104602973	0.0
28	2.2103111012875063E-4	0.0	0.0	0.036028070950986354	0.0
29	2.2103111012875063E-4	0.0	0.0	0.06597778637343206	0.0
30	2.2103111012875063E-4	0.0	0.0	0.10554235508647843	0.0
31	2.2103111012875063E-4	0.0	0.0	0.21550533237553185	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1245	0.0	18.72289	1
TTAGACT	235	0.0	17.319149	4
AGACTAT	150	2.5102054E-10	17.266666	6
GTACCGT	65	0.001580408	17.076923	6
GACAGGC	1170	0.0	16.760683	7
ATCGTAC	90	4.4482284E-5	16.444445	25
TTTAGCG	80	3.383908E-4	16.1875	26
CGCGAAC	160	6.2937033E-10	16.1875	28
TAGCGGG	70	0.0025932654	15.857142	28
GACGGTA	155	7.215931E-9	15.5161295	8
TGCATCA	1235	0.0	15.429151	14
CGTTATT	180	2.0190782E-10	15.416666	2
GTTCGCG	145	5.3505573E-8	15.310346	30
CGCGCTT	170	1.4879333E-9	15.235294	12
ATTAGAC	160	1.0975782E-8	15.031251	3
TCGCGGA	160	1.0975782E-8	15.031251	32
TATTAGA	285	0.0	14.929824	2
AAGACGG	300	0.0	14.8	5
GACGGAC	290	0.0	14.672415	7
GTATTAG	545	0.0	14.59633	1
>>END_MODULE