FastQCFastQC Report
Fri 10 Feb 2017
ERR1630315.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630315.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505342
Sequences flagged as poor quality0
Sequence length43
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT178853.539187322644862No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT158913.1446030608973725No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT105962.0967978121747253No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67871.34305084477443No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA25950.5135136204788044No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25000.49471447059615076No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21280.42110095737144354No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16890.33422909633475945No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA15410.3049419996754673No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13870.27446758828674445No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA13640.26991621515725983No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10900.2156955091799217No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA9660.19115767143835263No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA9380.18561686936767577No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9150.18106549623819115No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT9100.18007606729699885No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9050.17908663835580657No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA8790.17394160786160662No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA8610.1703796636733143No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA7530.1490079985435606No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA6930.13713485124925298No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6690.13238559233152994No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6500.12862576235499917No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6430.12724056183732999No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA6220.12308496028432231No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA5880.11635684348421464No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA5560.11002449826058391No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5400.10685832564876856No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5370.10626466828405318No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA5270.10428581040166857No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG5200.10290060988399935No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCAACC303.59665E-430.8333328
CCTAGAG250.00549346629.5999987
AAACGTA250.00549346629.59999815
GGTGAGC358.862491E-426.428578
ACGTAGG358.862491E-426.4285717
TGCCTGA451.3217793E-424.66666621
CTTGGAC551.8999248E-523.5454543
GATATAG400.001929698223.1252
CCTATCT400.001929698223.12530
ATGCCCC1054.0017767E-1122.9047637
GGCGTCG502.6991998E-422.1999998
TCTGACT603.72097E-521.58333223
ATATGCT603.72097E-521.58333235
TTAGGAC450.003822886420.5555553
TCACATT450.003822886420.55555526
TACGGGC450.003822886420.5555554
GCCTGAT555.137615E-420.18181822
GGAGGGG555.137615E-420.1818188
TGGACAA656.8923524E-519.9230775
CAGTCGG7250.019.64827510
Produced by FastQC (version 0.11.5)