##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630315.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	505342
Sequences flagged as poor quality	0
Sequence length	43
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.838149213799763	31.0	31.0	34.0	30.0	34.0
2	31.936072996109566	33.0	31.0	34.0	30.0	34.0
3	31.94641648626079	33.0	31.0	34.0	30.0	34.0
4	35.658506516379006	37.0	35.0	37.0	33.0	37.0
5	35.64382141203383	37.0	35.0	37.0	33.0	37.0
6	35.786594029389995	37.0	35.0	37.0	35.0	37.0
7	35.757870907227186	37.0	35.0	37.0	35.0	37.0
8	35.83511760352395	37.0	35.0	37.0	35.0	37.0
9	37.4288679745598	39.0	37.0	39.0	34.0	39.0
10	37.38133976594069	39.0	37.0	39.0	34.0	39.0
11	37.50009894289412	39.0	37.0	39.0	35.0	39.0
12	37.407933637022055	39.0	37.0	39.0	34.0	39.0
13	37.47213768101602	39.0	37.0	39.0	35.0	39.0
14	38.48024110404439	40.0	38.0	41.0	34.0	41.0
15	38.47233952452003	40.0	38.0	41.0	34.0	41.0
16	38.30816160145011	40.0	38.0	41.0	34.0	41.0
17	38.48439472673952	40.0	38.0	41.0	34.0	41.0
18	38.60658722211888	40.0	38.0	41.0	35.0	41.0
19	38.66999576524413	40.0	38.0	41.0	34.0	41.0
20	38.69831717925682	40.0	38.0	41.0	35.0	41.0
21	38.679211306402394	40.0	38.0	41.0	35.0	41.0
22	38.625910769340365	40.0	38.0	41.0	34.0	41.0
23	38.528281836855044	40.0	38.0	41.0	34.0	41.0
24	38.4750386866716	40.0	38.0	41.0	34.0	41.0
25	38.41536029065465	40.0	38.0	41.0	34.0	41.0
26	38.133830158585674	40.0	37.0	41.0	34.0	41.0
27	37.91705815071773	40.0	36.0	41.0	33.0	41.0
28	37.729300157121315	40.0	36.0	41.0	33.0	41.0
29	37.52210977911988	39.0	35.0	41.0	33.0	41.0
30	37.244493827942264	39.0	35.0	41.0	33.0	41.0
31	36.88170387579105	39.0	35.0	40.0	33.0	41.0
32	36.559140146672945	38.0	35.0	40.0	32.0	41.0
33	36.29429376541036	38.0	35.0	40.0	31.0	41.0
34	36.04166287385573	38.0	35.0	40.0	31.0	41.0
35	35.79659517712757	38.0	35.0	40.0	30.0	41.0
36	35.46976503041505	38.0	35.0	40.0	29.0	41.0
37	35.161229424825166	37.0	35.0	40.0	27.0	41.0
38	34.81307312671419	37.0	35.0	40.0	25.0	41.0
39	34.40132820941066	37.0	34.0	40.0	22.0	41.0
40	33.95548163421999	37.0	33.0	40.0	20.0	41.0
41	33.45263207886936	36.0	33.0	40.0	15.0	41.0
42	32.977769510549294	36.0	33.0	40.0	12.0	41.0
43	31.86281765616157	35.0	31.0	39.0	9.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	10.0
11	1.0
12	1.0
13	2.0
14	5.0
15	2.0
16	2.0
17	10.0
18	25.0
19	55.0
20	104.0
21	220.0
22	459.0
23	765.0
24	1325.0
25	2142.0
26	3361.0
27	4849.0
28	6742.0
29	8960.0
30	11339.0
31	14015.0
32	17624.0
33	22454.0
34	29045.0
35	37456.0
36	52180.0
37	88813.0
38	98461.0
39	104912.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.39008433892295	19.70744565066826	14.619406263480968	18.283063746927823
2	18.37725738212933	26.350867333409848	32.52173775383799	22.750137530622826
3	22.347835723134036	27.72478836114948	31.32749702181889	18.599878893897596
4	14.181287128321019	19.308705787367764	31.26259048327667	35.24741660103455
5	11.929742629743817	42.4041540184667	30.825065005481438	14.841038346308045
6	28.41244147527813	32.60583921383934	18.981996350986066	19.999722959896467
7	24.26099552382347	36.40208017540596	21.52997376034448	17.806950540426087
8	31.706646192083777	31.460080499938652	19.624729391184584	17.208543916792983
9	28.880639250250322	12.590681162460275	22.47191011235955	36.05676947492985
10	21.68669930462934	30.807453170328213	26.40390072465776	21.10194680038469
11	28.815139054343398	27.163584265705204	18.355094173846624	25.66618250610478
12	20.28843832493638	33.8327311009178	27.731318592161347	18.147511981984476
13	31.64292696827099	20.72655746009633	28.1514301205916	19.47908545104108
14	22.70719631457508	25.466317859983935	30.281076973613906	21.54540885182708
15	28.506635110479632	27.014774152949883	26.55389815214251	17.92469258442797
16	19.06886821202275	27.168531410411166	32.304657044140406	21.457943333425682
17	17.857213530638656	31.817660119285552	30.519331462653014	19.805794887422774
18	16.92992072695323	25.37845657000605	34.76180487669737	22.92981782634335
19	17.59066137388145	29.65120650965089	37.40278860652786	15.355343509939804
20	18.951126168020867	25.645008726763262	38.775720205326294	16.62814489988958
21	20.938097367723245	26.115581131194322	36.393768972299945	16.55255252878249
22	20.657891091577586	26.844196603488328	34.82532621472191	17.67258609021217
23	18.770852214935626	28.244238555275437	35.20130921237499	17.78360001741395
24	17.076949867614406	29.931214900008314	36.068246850647675	16.9235883817296
25	17.145418350344915	29.600349862073607	35.56640849167494	17.687823295906536
26	18.638466622604096	29.002734781593453	35.36515864503643	16.993639950766017
27	17.139086005121285	29.94645210570267	35.154014508985995	17.76044738019005
28	16.32894158807303	30.862663305246745	36.38783239865279	16.420562708027433
29	16.50980919852298	30.949733052071664	36.059737761753425	16.480719987651927
30	15.368799743540018	32.824107238266365	36.8138805007302	14.99321251746342
31	16.31449592553162	32.132892179949415	35.113052150820636	16.439559743698325
32	14.586952994209861	32.92166493186792	35.85631117144429	16.635070902477924
33	15.4788242418006	32.28724309477542	36.393175314935235	15.840757348488745
34	15.357520253610426	32.861111880666954	34.4208476635625	17.36052020216012
35	15.517609856295342	33.46802759319431	34.37929164803242	16.635070902477924
36	14.658389763763946	33.617827134890824	34.228700563182954	17.495082538162272
37	15.280740567773904	33.6953983638803	34.11709297861646	16.906768089729333
38	15.270648392573744	32.410921712424454	34.50969838248157	17.808731512520236
39	15.735482109145885	31.872870254204084	35.23653288268143	17.1551147539686
40	15.294988344527072	32.21897249783315	34.71114611490832	17.774893042731456
41	14.310308662252494	32.47523459360196	35.05942510220801	18.155031641937537
42	14.176339983615058	32.56764725670932	34.66840278464881	18.587609975026815
43	13.968559905964673	33.13795409841256	34.54571359594097	18.3477723996818
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	89.0
1	193.0
2	297.0
3	1058.5
4	1820.0
5	1820.0
6	2630.5
7	3441.0
8	3441.5
9	3442.0
10	4990.0
11	6538.0
12	6538.0
13	12699.5
14	18861.0
15	27503.5
16	36146.0
17	30689.0
18	25232.0
19	25232.0
20	29391.0
21	33550.0
22	22877.5
23	12205.0
24	11743.0
25	11281.0
26	11281.0
27	11510.5
28	11740.0
29	11786.5
30	11833.0
31	12037.0
32	12241.0
33	12241.0
34	13260.0
35	14279.0
36	14430.5
37	14582.0
38	16051.0
39	17520.0
40	17520.0
41	19714.0
42	21908.0
43	24184.5
44	26461.0
45	30773.0
46	35085.0
47	35085.0
48	41103.5
49	47122.0
50	41973.5
51	36825.0
52	31280.0
53	25735.0
54	25735.0
55	23447.5
56	21160.0
57	19274.0
58	17388.0
59	15072.5
60	12757.0
61	12757.0
62	10756.0
63	8755.0
64	7372.5
65	5990.0
66	5009.0
67	4028.0
68	4028.0
69	3463.5
70	2899.0
71	2279.5
72	1660.0
73	1300.5
74	941.0
75	941.0
76	762.5
77	584.0
78	467.5
79	351.0
80	273.5
81	196.0
82	196.0
83	185.0
84	174.0
85	141.0
86	108.0
87	84.5
88	61.0
89	61.0
90	52.0
91	43.0
92	25.5
93	8.0
94	6.5
95	5.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	505342.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.467397681290535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.69023082075347	21.842147722794074
2	12.284446307789503	7.485502219077679
3	5.013299816508913	4.582265976153537
4	2.5975464000405593	3.165619166625612
5	1.616881856404503	2.4631091261369646
6	1.0876911697792435	1.988347165445539
7	0.789868801926673	1.6845672833041114
8	0.6267665300354268	1.52767560991295
9	0.4778083506716227	1.3101819331818467
>10	3.26902597867308	19.59537790773439
>50	0.3239572806829744	6.816537418727122
>100	0.20231067126988966	11.856450483037067
>500	0.012359815929671714	2.7097287119133897
>1k	0.005204133023019669	2.833006305448361
>5k	6.505166278774586E-4	1.345152777044776
>10k+	0.0019515498836323762	8.794330193462619
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17885	3.539187322644862	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15891	3.1446030608973725	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10596	2.0967978121747253	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6787	1.34305084477443	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	2595	0.5135136204788044	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2500	0.49471447059615076	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2128	0.42110095737144354	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1689	0.33422909633475945	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	1541	0.3049419996754673	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1387	0.27446758828674445	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1364	0.26991621515725983	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1090	0.2156955091799217	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	966	0.19115767143835263	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	938	0.18561686936767577	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	915	0.18106549623819115	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	910	0.18007606729699885	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	905	0.17908663835580657	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	879	0.17394160786160662	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	861	0.1703796636733143	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	753	0.1490079985435606	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	693	0.13713485124925298	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	669	0.13238559233152994	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	650	0.12862576235499917	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	643	0.12724056183732999	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	622	0.12308496028432231	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	588	0.11635684348421464	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	556	0.11002449826058391	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	540	0.10685832564876856	No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	537	0.10626466828405318	No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA	527	0.10428581040166857	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	520	0.10290060988399935	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.9788578823846028E-4	0.0
9	0.0	0.0	0.0	1.9788578823846028E-4	0.0
10	0.0	0.0	0.0	1.9788578823846028E-4	0.0
11	0.0	0.0	0.0	1.9788578823846028E-4	0.0
12	0.0	0.0	0.0	1.9788578823846028E-4	0.0
13	0.0	0.0	0.0	1.9788578823846028E-4	0.0
14	0.0	0.0	0.0	5.936573647153809E-4	0.0
15	0.0	0.0	0.0	0.0025725152470999837	0.0
16	0.0	0.0	0.0	0.006332345223630729	0.0
17	0.0	0.0	0.0	0.012664690447261458	0.0
18	0.0	0.0	0.0	0.014643548329646061	0.0
19	0.0	0.0	0.0	0.02077800776503833	0.0
20	0.0	0.0	0.0	0.023548408800376775	0.0
21	0.0	0.0	0.0	0.03185961190639211	0.0
22	0.0	0.0	0.0	0.046107388659561245	0.0
23	0.0	0.0	0.0	0.06708328221283803	0.0
24	0.0	0.0	0.0	0.09993232306042245	0.0
25	0.0	0.0	0.0	0.11101392720177622	0.0
26	0.0	0.0	0.0	0.13179193496681454	0.0
27	0.0	0.0	0.0	0.15474668640247594	0.0
28	0.0	0.0	0.0	0.19689635929726798	0.0
29	0.0	0.0	0.0	0.2705098725219752	0.0
30	0.0	0.0	0.0	0.4224861578891127	0.0
31	0.0	0.0	0.0	0.8075719018011565	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCAACC	30	3.59665E-4	30.833332	8
CCTAGAG	25	0.005493466	29.599998	7
AAACGTA	25	0.005493466	29.599998	15
GGTGAGC	35	8.862491E-4	26.42857	8
ACGTAGG	35	8.862491E-4	26.42857	17
TGCCTGA	45	1.3217793E-4	24.666666	21
CTTGGAC	55	1.8999248E-5	23.545454	3
GATATAG	40	0.0019296982	23.125	2
CCTATCT	40	0.0019296982	23.125	30
ATGCCCC	105	4.0017767E-11	22.90476	37
GGCGTCG	50	2.6991998E-4	22.199999	8
TCTGACT	60	3.72097E-5	21.583332	23
ATATGCT	60	3.72097E-5	21.583332	35
TTAGGAC	45	0.0038228864	20.555555	3
TCACATT	45	0.0038228864	20.555555	26
TACGGGC	45	0.0038228864	20.555555	4
GCCTGAT	55	5.137615E-4	20.181818	22
GGAGGGG	55	5.137615E-4	20.181818	8
TGGACAA	65	6.8923524E-5	19.923077	5
CAGTCGG	725	0.0	19.648275	10
>>END_MODULE