FastQCFastQC Report
Fri 10 Feb 2017
ERR1630314.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630314.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2278177
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42430.1862454058661816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37660.16530761218289888No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29770.1306746578514312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19550.016.9386181
AATAACG1251.6597005E-716.2799992
AACCGTT803.38439E-416.18757
CTTATAC9450.015.46560937
CGATCGC1201.937433E-615.41666719
CCGATCG1252.9616513E-614.79999918
TATACTG2500.014.7999995
TCTTATA15300.014.26797437
TTACTCG1051.6567581E-414.09523819
TTAGTAC1458.920251E-714.0344833
TCTATAC2405.456968E-1213.8750013
CTATACT3850.013.4545474
CGAAATA1409.5678715E-613.21428524
GACGGAC2102.044544E-913.2142857
CTAGACT2254.0745363E-1013.1555564
ATTCCGC4250.013.05882428
TCGCACG1703.7356403E-713.05882322
AGACCGT1857.392555E-813.06
GAACCGT1000.001833440912.9500016
CTATACA2305.7661964E-1012.8695644
Produced by FastQC (version 0.11.5)