##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630314.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2278177
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96137262381281	33.0	31.0	34.0	30.0	34.0
2	32.16223190735399	33.0	31.0	34.0	30.0	34.0
3	32.310416618199554	34.0	31.0	34.0	30.0	34.0
4	35.93684643467123	37.0	35.0	37.0	35.0	37.0
5	35.851371952223204	37.0	35.0	37.0	35.0	37.0
6	35.91254981504949	37.0	35.0	37.0	35.0	37.0
7	35.88759345740037	37.0	35.0	37.0	35.0	37.0
8	35.87119657515636	37.0	35.0	37.0	35.0	37.0
9	37.598924929889115	39.0	37.0	39.0	35.0	39.0
10	37.512681411497	39.0	37.0	39.0	35.0	39.0
11	37.59211861062595	39.0	37.0	39.0	35.0	39.0
12	37.48635465988815	39.0	37.0	39.0	35.0	39.0
13	37.52023569722633	39.0	37.0	39.0	35.0	39.0
14	38.78290668372124	40.0	38.0	41.0	35.0	41.0
15	38.783414106981155	40.0	38.0	41.0	35.0	41.0
16	38.76909827462923	40.0	38.0	41.0	35.0	41.0
17	38.73358830327933	40.0	38.0	41.0	35.0	41.0
18	38.70915824363076	40.0	38.0	41.0	35.0	41.0
19	38.73655471019153	40.0	38.0	41.0	35.0	41.0
20	38.69044766934264	40.0	38.0	41.0	34.0	41.0
21	38.6559086497669	40.0	38.0	41.0	34.0	41.0
22	38.62954897709879	40.0	38.0	41.0	34.0	41.0
23	38.58627490313527	40.0	38.0	41.0	34.0	41.0
24	38.59107611041635	40.0	38.0	41.0	34.0	41.0
25	38.55610516654325	40.0	38.0	41.0	34.0	41.0
26	38.426697749999235	40.0	38.0	41.0	34.0	41.0
27	38.34524534309669	40.0	38.0	41.0	34.0	41.0
28	38.27767640530126	40.0	38.0	41.0	34.0	41.0
29	38.22914944712373	40.0	38.0	41.0	34.0	41.0
30	38.182950666256396	40.0	38.0	41.0	34.0	41.0
31	38.08768238815509	40.0	38.0	41.0	33.0	41.0
32	38.00613560754937	40.0	37.0	41.0	33.0	41.0
33	37.95713283032881	40.0	37.0	41.0	33.0	41.0
34	37.8777935164827	40.0	37.0	41.0	33.0	41.0
35	37.81987396062729	40.0	37.0	41.0	33.0	41.0
36	37.73002141624641	40.0	37.0	41.0	33.0	41.0
37	37.68233811508061	40.0	37.0	41.0	33.0	41.0
38	37.59712305057948	40.0	37.0	41.0	32.0	41.0
39	37.51117099329859	40.0	37.0	41.0	32.0	41.0
40	37.442738206908416	40.0	37.0	41.0	32.0	41.0
41	37.34790009731465	40.0	37.0	41.0	32.0	41.0
42	37.30922312006486	40.0	36.0	41.0	31.0	41.0
43	36.15299733076051	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	4.0
11	1.0
12	5.0
13	1.0
14	5.0
15	2.0
16	10.0
17	31.0
18	54.0
19	115.0
20	226.0
21	432.0
22	824.0
23	1454.0
24	2570.0
25	4199.0
26	6496.0
27	9842.0
28	14453.0
29	20882.0
30	28987.0
31	39467.0
32	51993.0
33	69888.0
34	94677.0
35	130030.0
36	185980.0
37	285394.0
38	541810.0
39	788339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.847319150355744	19.027845509808937	12.626762538643838	26.49807280119148
2	17.603548802397707	20.367205884354025	36.18972538130269	25.83951993194559
3	19.13196384653168	22.339001754472985	30.189050280114323	28.339984118881016
4	14.256311076795175	15.536808597400464	34.972216820729905	35.23466350507445
5	14.401866053427806	36.41016479404366	34.780660150637985	14.407309001890548
6	34.76981814845818	35.41809964721793	15.667702728980231	14.144379475343664
7	29.80958020382086	30.61864815595979	20.966588636440452	18.6051830037789
8	26.90712793606467	35.14274790764721	19.516876871287874	18.433247285000242
9	26.588583766757367	14.873954043079182	19.412187902871462	39.12527428729199
10	16.459212782852255	27.40634287853841	33.39758060940831	22.736863729201023
11	36.00914239762758	21.80796312139048	21.52738790708536	20.655506573896584
12	21.11030003375506	25.31875266934922	29.866292215222963	23.704655081672758
13	30.179832383524197	19.70759954121212	24.71682402201409	25.39574405324959
14	22.32385806721778	20.56719034561406	25.330253092714045	31.778698494454115
15	25.10042020440027	27.88264476377384	22.275881110203464	24.74105392162242
16	24.623854950690838	27.071250390114553	23.72063276909564	24.58426189009897
17	23.536011468819147	27.03424711951705	25.497623757943305	23.932117653720496
18	23.251178464184303	25.978359012491126	26.561105655969662	24.209356867354906
19	24.301491938510484	25.926431528366763	26.49047901019104	23.281597522931712
20	24.447354178362787	25.599415673145675	26.484026482577956	23.46920366591358
21	24.502661557903537	25.628078942066395	25.743127070460286	24.126132429569783
22	24.37589353241649	25.690892323116245	25.89693425927836	24.0362798851889
23	23.985976506654225	25.84843056531604	25.854444145472456	24.311148782557282
24	24.360135318721944	25.817045822163948	25.797732134070355	24.02508672504375
25	24.591680102116737	25.426426480471008	25.95219774407344	24.029695673338814
26	24.453982284958546	25.60867746448147	25.84891340751838	24.088426843041606
27	24.29095719955034	25.69580853463098	25.863969305282247	24.149264960536428
28	23.894763225157657	25.834910983650524	26.086735139543592	24.183590651648228
29	23.699168238464352	26.13449262282957	26.30546265720354	23.860876481502537
30	23.72462719095136	26.46045500415464	25.79356213323197	24.021355671662032
31	24.20979581481158	25.95987932456521	25.75331065145509	24.07701420916812
32	23.55730042046777	26.049380710980753	26.075980926855113	24.317337941696366
33	23.55115515607435	25.7000663249607	26.486133430369986	24.26264508859496
34	24.14009095869197	25.40526921305939	26.12988367453451	24.324756153714134
35	24.03566535874956	25.48230449170543	26.425075839146828	24.056954310398183
36	24.025042830298084	25.735313805731515	26.038407024563938	24.201236339406464
37	24.32927731251786	25.030934822008998	26.31950897581707	24.320278889656073
38	23.73634708804452	25.500301337429004	26.809681600683355	23.95366997384312
39	23.520648307835607	25.21990170210655	27.151051037737627	24.108398952320208
40	23.296302262730244	24.747682028218176	27.592193231693585	24.363822477357992
41	22.83215922204464	25.138652527876452	27.836906438788557	24.192281811290343
42	23.366577750543527	24.69369149104745	27.7167226251516	24.223008133257427
43	22.907965447812	24.660682642305666	27.760441791836193	24.670910118046137
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	105.0
1	127.5
2	150.0
3	386.0
4	622.0
5	622.0
6	833.0
7	1044.0
8	994.5
9	945.0
10	1372.0
11	1799.0
12	1799.0
13	3146.0
14	4493.0
15	7431.0
16	10369.0
17	10594.0
18	10819.0
19	10819.0
20	13706.0
21	16593.0
22	18516.0
23	20439.0
24	24653.0
25	28867.0
26	28867.0
27	34867.0
28	40867.0
29	49444.0
30	58021.0
31	64603.5
32	71186.0
33	71186.0
34	80655.0
35	90124.0
36	97398.0
37	104672.0
38	114983.0
39	125294.0
40	125294.0
41	133919.5
42	142545.0
43	143782.5
44	145020.0
45	150357.5
46	155695.0
47	155695.0
48	161052.0
49	166409.0
50	168690.0
51	170971.0
52	173955.0
53	176939.0
54	176939.0
55	164532.0
56	152125.0
57	144090.5
58	136056.0
59	127331.0
60	118606.0
61	118606.0
62	108710.0
63	98814.0
64	86846.5
65	74879.0
66	64437.5
67	53996.0
68	53996.0
69	45380.0
70	36764.0
71	30998.0
72	25232.0
73	20407.0
74	15582.0
75	15582.0
76	12678.0
77	9774.0
78	7838.5
79	5903.0
80	4762.5
81	3622.0
82	3622.0
83	2484.0
84	1346.0
85	1079.5
86	813.0
87	595.0
88	377.0
89	377.0
90	305.5
91	234.0
92	140.0
93	46.0
94	29.5
95	13.0
96	13.0
97	8.5
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2278177.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.43090687129635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.99608959330095	45.14251881907445
2	11.717060369705154	13.224086850563774
3	3.557578959736211	6.022722208924724
4	1.4734961883397903	3.3260290471765144
5	0.8193284576913993	2.3117723946493114
6	0.4849171479278329	1.6418588649006107
7	0.32176832160768526	1.2710374733543635
8	0.2360747416201384	1.0657529407225104
9	0.1722544413884405	0.8748426906142436
>10	1.032837570473675	11.071886887376339
>50	0.10965415116069441	4.34104027238409
>100	0.075128262317942	7.714026779226138
>500	0.0028782939798483063	1.0232319604294429
>1k	9.335007502210029E-4	0.9691928106036231
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4243	0.1862454058661816	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3766	0.16530761218289888	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2977	0.1306746578514312	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.3894745667259394E-5	0.0
10	0.0	0.0	0.0	1.3168423700177817E-4	0.0
11	0.0	0.0	0.0	1.3168423700177817E-4	0.0
12	0.0	0.0	0.0	1.3168423700177817E-4	0.0
13	0.0	0.0	0.0	1.7557898266903758E-4	0.0
14	0.0	0.0	0.0	2.6336847400355634E-4	0.0
15	0.0	0.0	0.0	3.072632196708157E-4	0.0
16	0.0	0.0	0.0	5.267369480071127E-4	0.0
17	0.0	0.0	0.0	7.462106763434096E-4	0.0
18	0.0	0.0	0.0	7.462106763434096E-4	0.0
19	0.0	0.0	0.0	8.340001676779284E-4	0.0
20	0.0	0.0	0.0	9.217896590124473E-4	0.0
21	0.0	0.0	0.0	0.001097368641681485	0.0
22	0.0	0.0	0.0	0.0014924213526868193	0.0
23	0.0	0.0	0.0	0.0020630530463611914	0.0
24	0.0	4.3894745667259394E-5	0.0	0.002765368977037342	0.0
25	0.0	4.3894745667259394E-5	0.0	0.0031604216880426763	0.0
26	0.0	4.3894745667259394E-5	0.0	0.004784527277731274	0.0
27	0.0	4.3894745667259394E-5	0.0	0.01018358099480418	0.0
28	0.0	8.778949133451879E-5	0.0	0.0273464265507026	0.0
29	0.0	8.778949133451879E-5	0.0	0.05337601073138742	0.0
30	0.0	8.778949133451879E-5	0.0	0.09362749250826428	0.0
31	0.0	8.778949133451879E-5	0.0	0.25094626097972195	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1955	0.0	16.938618	1
AATAACG	125	1.6597005E-7	16.279999	2
AACCGTT	80	3.38439E-4	16.1875	7
CTTATAC	945	0.0	15.465609	37
CGATCGC	120	1.937433E-6	15.416667	19
CCGATCG	125	2.9616513E-6	14.799999	18
TATACTG	250	0.0	14.799999	5
TCTTATA	1530	0.0	14.267974	37
TTACTCG	105	1.6567581E-4	14.095238	19
TTAGTAC	145	8.920251E-7	14.034483	3
TCTATAC	240	5.456968E-12	13.875001	3
CTATACT	385	0.0	13.454547	4
CGAAATA	140	9.5678715E-6	13.214285	24
GACGGAC	210	2.044544E-9	13.214285	7
CTAGACT	225	4.0745363E-10	13.155556	4
ATTCCGC	425	0.0	13.058824	28
TCGCACG	170	3.7356403E-7	13.058823	22
AGACCGT	185	7.392555E-8	13.0	6
GAACCGT	100	0.0018334409	12.950001	6
CTATACA	230	5.7661964E-10	12.869564	4
>>END_MODULE