FastQCFastQC Report
Fri 10 Feb 2017
ERR1630313.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630313.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200778
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70203.496399007859427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70103.4914183824921055No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42512.1172638436482085No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28771.4329259181782865No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA11510.573269979778661No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10150.5055334747830937No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9000.44825628305890086No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA8070.4019364671428145No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6590.3282232117064619No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6130.3053123350167847No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5350.26646345715167996No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA4790.23857195509468168No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4670.23259520465389633No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4490.22363007899271833No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA4310.21466495333154031No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4150.20669595274382652No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4120.2052017651336302No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA4070.20271145244996963No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4000.19922501469284482No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA3920.19524051439898793No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3610.17980057576029246No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3460.17232963770931078No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.17232963770931078No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3420.17033738756238231No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA3410.16983932502565022No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT3400.1693412624889181No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3360.16734901234198965No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT3330.16585482473179333No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3310.1648586996583291No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3100.15439938638695475No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3090.15390132385022262No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2930.14593232326250885No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2870.14294394804211616No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2810.1399555728217235No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2640.1314885096972776No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA2530.12600982179322434No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2470.12302144657283169No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2450.12202532149936747No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA2430.12102919642590322No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA2360.11754275866877845No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA2350.11704469613204635No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA2330.11604857105858213No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT2270.11306019583818944No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2260.11256213330145733No Hit
GGTATCAACGCAGAGTACATGGGAATGGTATCAACGCAAAAAA2220.11056988315452888No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA2200.10957375808106466No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2180.10857763300760044No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG2160.10758150793413622No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT2080.10359700764027932No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG2070.1030989451035472No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2040.10160475749335088No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG2010.10011056988315453No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCCTA200.0018383237.029
GAGTCCT200.0018383237.028
GTCCTAC200.0018383237.030
CCAATGC200.0018383237.018
CTACACT200.0018383237.033
CAATGCG200.0018383237.019
TAGCACA352.378573E-531.7142854
CACACAT352.378573E-531.7142857
TCGTGGT303.5889968E-430.83333435
TCCAATG303.5889968E-430.83333417
TGAATGG303.5889968E-430.83333429
CGACGAG250.005485677629.59999824
CCTACAC250.005485677629.59999832
TAGCCCG250.005485677629.5999985
TCCTACA250.005485677629.59999831
GTGCTAG250.005485677629.5999981
TGCGACG250.005485677629.59999822
TCATCCA250.005485677629.59999814
CGACTAT250.005485677629.59999836
GGCGACT250.005485677629.59999834
Produced by FastQC (version 0.11.5)