FastQCFastQC Report
Fri 10 Feb 2017
ERR1630312.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630312.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145471
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC138170.6440077726522521No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT83150.3875605869293969No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT54310.2531378890695796No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG52560.24498117196643535No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG48170.22451946449054777No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG47650.22209575426561348No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT46160.2151508922749364No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT45430.21174837599762475No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG44810.20885856765251082No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT41680.19426969649088707No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC41550.1936637689346535No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG38960.18159182762200002No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT36440.1698461549934723No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG36420.16975293536943636No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG35990.16774871345266376No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT35070.1634606107470108No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC34420.16043097296584294No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG34350.16010470428171716No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG33660.15688862725247743No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT32220.15017681432189015No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA28980.1350752352280688No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA27670.1289693498537151No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA26570.12384227053173873No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT26450.12328295278752312No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG26140.12183804861496614No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT26120.1217448289909302No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA25260.117736385157385No Hit
TTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGATGCTGGTAC24770.11545250436850463No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC24250.11302879414357035No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG23580.10990593673836654No Hit
GTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCAA22750.10603732234087528No Hit
GTTCCACAATGCCACGCTTCTGCAGGGACCCCTCCAGGGCCAA22310.10398649061208472No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC21660.10095685283091685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGGC31550.020.9920757
TGCATCA31400.020.6210214
ACAGGCT32450.020.4668718
CCAGGAC34300.020.2798823
CAGGACA33850.020.0576084
TCTAGCG1303.274181E-1119.92307928
GCATCAG33200.019.83734915
TCCAGGA36000.019.5791662
GCTGCAT32950.019.53869412
CTGCATC34350.019.06550213
GGTATCA7800.018.974361
GGACAGG35950.018.8344926
GGGTACG500.00703682318.4999981
CTTATAC8100.018.0432137
GGCCATC35750.018.00839226
TAACGCC1653.6379788E-1217.9393924
CATCAGA37050.017.6261816
ATCAGAA37300.017.45844517
AAGAGGC37600.017.26994522
TATCCCG752.0683189E-417.26666610
Produced by FastQC (version 0.11.5)