##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630312.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2145471
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01592750496278	33.0	31.0	34.0	30.0	34.0
2	32.24621959467175	33.0	31.0	34.0	30.0	34.0
3	32.360473760773274	34.0	31.0	34.0	30.0	34.0
4	35.98282894525258	37.0	35.0	37.0	35.0	37.0
5	35.915969500403406	37.0	35.0	37.0	35.0	37.0
6	35.96516196210529	37.0	35.0	37.0	35.0	37.0
7	35.947266124780995	37.0	35.0	37.0	35.0	37.0
8	35.94003088366144	37.0	35.0	37.0	35.0	37.0
9	37.717123186470474	39.0	37.0	39.0	35.0	39.0
10	37.62635989952789	39.0	37.0	39.0	35.0	39.0
11	37.68378598452274	39.0	37.0	39.0	35.0	39.0
12	37.620068507101706	39.0	37.0	39.0	35.0	39.0
13	37.601762969529766	39.0	37.0	39.0	35.0	39.0
14	38.90754710737176	40.0	38.0	41.0	36.0	41.0
15	38.92407774330206	40.0	38.0	41.0	36.0	41.0
16	38.8954635136061	40.0	38.0	41.0	35.0	41.0
17	38.85024267398627	40.0	38.0	41.0	35.0	41.0
18	38.73179921798057	40.0	38.0	41.0	35.0	41.0
19	38.754376544823955	40.0	38.0	41.0	35.0	41.0
20	38.74048262595952	40.0	38.0	41.0	35.0	41.0
21	38.697843503827364	40.0	38.0	41.0	35.0	41.0
22	38.64973285586242	40.0	38.0	41.0	34.0	41.0
23	38.599444597479994	40.0	38.0	41.0	34.0	41.0
24	38.56233386515129	40.0	38.0	41.0	34.0	41.0
25	38.542875200830025	40.0	38.0	41.0	34.0	41.0
26	38.37819714179311	40.0	38.0	41.0	34.0	41.0
27	38.28014734293775	40.0	38.0	41.0	34.0	41.0
28	38.20585922624915	40.0	38.0	41.0	34.0	41.0
29	38.14871000353769	40.0	38.0	41.0	33.0	41.0
30	38.10402191406922	40.0	38.0	41.0	33.0	41.0
31	38.08700140901462	40.0	38.0	41.0	33.0	41.0
32	38.015728481065466	40.0	38.0	41.0	33.0	41.0
33	37.93284178625579	40.0	37.0	41.0	33.0	41.0
34	37.8525969355913	40.0	37.0	41.0	33.0	41.0
35	37.71760606412298	40.0	37.0	41.0	33.0	41.0
36	37.65009081921872	40.0	37.0	41.0	33.0	41.0
37	37.575241520393426	40.0	37.0	41.0	32.0	41.0
38	37.492884779146394	40.0	37.0	41.0	32.0	41.0
39	37.42450818491604	40.0	37.0	41.0	32.0	41.0
40	37.309777666535695	40.0	36.0	41.0	31.0	41.0
41	37.18485078567829	39.0	36.0	41.0	31.0	41.0
42	37.17101699347136	39.0	36.0	41.0	31.0	41.0
43	35.90528466709641	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	4.0
11	1.0
12	1.0
13	0.0
14	1.0
15	4.0
16	16.0
17	36.0
18	89.0
19	152.0
20	287.0
21	534.0
22	1024.0
23	1722.0
24	2792.0
25	4430.0
26	6541.0
27	9575.0
28	13575.0
29	19126.0
30	26143.0
31	35348.0
32	47549.0
33	64548.0
34	90481.0
35	124955.0
36	171918.0
37	267189.0
38	515993.0
39	741433.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.0273818662662	16.877133272833795	11.564220630341776	28.531264230558236
2	19.68006092834627	18.454782190017948	34.63435301619085	27.23080386544493
3	20.969847646507457	20.840738467217687	27.908184263502044	30.28122962277281
4	16.028508425422668	14.089447025851202	31.691922193308603	38.19012235541753
5	16.478712599704217	35.110006147834206	32.09472418876788	16.316557063693708
6	38.2359864104432	31.590685681605578	14.821547343217409	15.351780564733804
7	32.0724913084353	28.02410286599073	19.805534542298638	20.097871283275328
8	28.584912124190915	32.93118387524231	18.517658826430186	19.96624517413659
9	28.273232311226764	13.646327542996387	17.358146532859216	40.72229361291763
10	18.780165287715377	25.250306342989486	30.882449587992568	25.08707878130257
11	37.82325652502411	20.387784314027083	19.935156429520603	21.853802731428203
12	24.523566154005344	22.68182604192739	27.578046964978785	25.21656083908848
13	31.285531242323945	17.903481333469433	23.458811608266902	27.352175815939717
14	23.977858474898987	18.629568985085328	23.660026166748466	33.73254637326722
15	27.156321385840222	25.064752681345958	20.45168636630372	27.3272395665101
16	26.52830077871013	24.145793627599723	21.16696054153144	28.158945052158714
17	25.514350928071273	24.81119530396822	23.83700362298069	25.83745014497982
18	25.576714856551312	22.87534998142599	24.495600266794565	27.052334895228135
19	26.65088458431738	22.988798263877722	23.57701409154447	26.783303060260426
20	26.06402044119916	23.998226962750838	23.638911921904327	26.29884067414568
21	28.625369441022507	22.77970664716512	22.646915292725932	25.948008619086437
22	27.747054143355935	23.66701763855116	22.81321910200604	25.77270911608686
23	26.96372964258198	23.95185019979296	22.49524696441947	26.589173193205596
24	27.397107674725035	23.34769381641607	23.185631500029597	26.069567008829296
25	26.93338665495828	22.986607602712876	23.616399382699647	26.463606359629193
26	27.68044872198226	23.335388826043328	23.737584893946366	25.24657755802805
27	28.120445347431872	23.025899674244023	22.40668832158533	26.446966656738773
28	26.34433185067521	22.30675688461881	24.18494586969481	27.163965395011164
29	26.31165837245062	22.505640952499476	24.597116437369696	26.58558423768021
30	25.897670022107032	23.214622803104774	24.156234225491747	26.731472949296446
31	26.037779117033043	23.2979611469929	23.894566740822878	26.76969299515118
32	25.623464498005333	23.1080727728317	24.294245878876946	26.97421685028602
33	27.004886106593844	23.34680822998773	23.392206186893226	26.2560994765252
34	27.152359551818687	23.656157552350976	23.431358428988318	25.76012446684201
35	27.418921066749448	23.014899758607786	24.13092509756599	25.43525407707678
36	27.012623335388824	23.582514049362587	22.46975139724564	26.935111218002945
37	25.744230520943884	23.08742462610774	24.06618406867303	27.10216078427534
38	27.100762489914803	22.731698540786617	24.195899175519035	25.97163979377955
39	25.079481381943637	23.140093713688042	24.747293251691588	27.033131652676733
40	26.150761301364593	21.69020229124514	25.880564221096442	26.27847218629383
41	24.646429618484706	22.13183026011538	26.393225543482064	26.828514577917854
42	24.790267498372152	22.945870627009175	25.08227797066472	27.18158390395396
43	24.816741871598357	20.79734473222896	26.814951122620627	27.570962273552052
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	204.0
1	161.0
2	118.0
3	246.5
4	375.0
5	375.0
6	461.5
7	548.0
8	549.0
9	550.0
10	782.0
11	1014.0
12	1014.0
13	1784.5
14	2555.0
15	3601.5
16	4648.0
17	4882.0
18	5116.0
19	5116.0
20	6758.0
21	8400.0
22	9416.5
23	10433.0
24	13039.0
25	15645.0
26	15645.0
27	18861.5
28	22078.0
29	26632.0
30	31186.0
31	36563.5
32	41941.0
33	41941.0
34	49346.5
35	56752.0
36	65037.0
37	73322.0
38	79301.0
39	85280.0
40	85280.0
41	92497.5
42	99715.0
43	103913.0
44	108111.0
45	111885.0
46	115659.0
47	115659.0
48	118171.5
49	120684.0
50	133921.5
51	147159.0
52	160390.5
53	173622.0
54	173622.0
55	178568.5
56	183515.0
57	174681.0
58	165847.0
59	149128.5
60	132410.0
61	132410.0
62	130595.0
63	128780.0
64	113124.5
65	97469.0
66	83153.0
67	68837.0
68	68837.0
69	59773.0
70	50709.0
71	47325.0
72	43941.0
73	51238.5
74	58536.0
75	58536.0
76	55956.5
77	53377.0
78	40449.5
79	27522.0
80	16656.5
81	5791.0
82	5791.0
83	3763.0
84	1735.0
85	1342.0
86	949.0
87	737.5
88	526.0
89	526.0
90	412.0
91	298.0
92	191.0
93	84.0
94	50.5
95	17.0
96	17.0
97	13.0
98	9.0
99	6.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2145471.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.79965901009492
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.29474937839063	39.33660958608293
2	10.204251044131203	9.75519440725753
3	2.9133047050882825	4.177649144871752
4	1.3008112427070992	2.4871333535158864
5	0.7151972231482016	1.7093091695725402
6	0.46502261995572547	1.3336753599518782
7	0.32392453507750746	1.0838437625195871
8	0.24130685983886407	0.9227508493675577
9	0.16825616301051208	0.7238328496424481
>10	1.149688738851187	10.542624039332393
>50	0.11706133617946296	3.8599717798057145
>100	0.08173257559837879	7.436303314813188
>500	0.01234678901151731	4.2642759353110975
>1k	0.011954827455596126	10.829614950707303
>5k	2.939711669408883E-4	0.8900360422532868
>10k+	9.799038898029611E-5	0.6471754549949301
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	13817	0.6440077726522521	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	8315	0.3875605869293969	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	5431	0.2531378890695796	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	5256	0.24498117196643535	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	4817	0.22451946449054777	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	4765	0.22209575426561348	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	4616	0.2151508922749364	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	4543	0.21174837599762475	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	4481	0.20885856765251082	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	4168	0.19426969649088707	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	4155	0.1936637689346535	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	3896	0.18159182762200002	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	3644	0.1698461549934723	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	3642	0.16975293536943636	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	3599	0.16774871345266376	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	3507	0.1634606107470108	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	3442	0.16043097296584294	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	3435	0.16010470428171716	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	3366	0.15688862725247743	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	3222	0.15017681432189015	No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA	2898	0.1350752352280688	No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA	2767	0.1289693498537151	No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA	2657	0.12384227053173873	No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT	2645	0.12328295278752312	No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG	2614	0.12183804861496614	No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT	2612	0.1217448289909302	No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA	2526	0.117736385157385	No Hit
TTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGATGCTGGTAC	2477	0.11545250436850463	No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC	2425	0.11302879414357035	No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG	2358	0.10990593673836654	No Hit
GTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCAA	2275	0.10603732234087528	No Hit
GTTCCACAATGCCACGCTTCTGCAGGGACCCCTCCAGGGCCAA	2231	0.10398649061208472	No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC	2166	0.10095685283091685	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.660981201796715E-5
2	0.0	0.0	0.0	0.0	4.660981201796715E-5
3	0.0	0.0	0.0	0.0	9.32196240359343E-5
4	0.0	0.0	0.0	0.0	9.32196240359343E-5
5	1.3982943605390146E-4	0.0	0.0	0.0	9.32196240359343E-5
6	1.3982943605390146E-4	0.0	0.0	0.0	1.3982943605390146E-4
7	1.3982943605390146E-4	0.0	0.0	0.0	1.3982943605390146E-4
8	1.3982943605390146E-4	0.0	0.0	0.0	1.864392480718686E-4
9	1.3982943605390146E-4	0.0	0.0	4.660981201796715E-5	1.864392480718686E-4
10	1.3982943605390146E-4	0.0	0.0	4.660981201796715E-5	1.864392480718686E-4
11	1.864392480718686E-4	0.0	0.0	4.660981201796715E-5	1.864392480718686E-4
12	1.864392480718686E-4	0.0	0.0	1.3982943605390146E-4	1.864392480718686E-4
13	2.3304906008983576E-4	0.0	0.0	1.3982943605390146E-4	1.864392480718686E-4
14	2.3304906008983576E-4	0.0	0.0	1.864392480718686E-4	1.864392480718686E-4
15	2.3304906008983576E-4	0.0	0.0	2.796588721078029E-4	1.864392480718686E-4
16	2.3304906008983576E-4	0.0	0.0	5.127079321976387E-4	2.796588721078029E-4
17	2.3304906008983576E-4	0.0	0.0	6.525373682515401E-4	2.796588721078029E-4
18	2.796588721078029E-4	0.0	0.0	7.457569922874744E-4	3.2626868412577003E-4
19	2.796588721078029E-4	0.0	0.0	9.32196240359343E-4	3.2626868412577003E-4
20	2.796588721078029E-4	0.0	0.0	0.0013050747365030801	3.2626868412577003E-4
21	3.2626868412577003E-4	0.0	0.0	0.0016779532326468174	5.127079321976387E-4
22	3.2626868412577003E-4	0.0	0.0	0.002097441540808522	5.127079321976387E-4
23	3.2626868412577003E-4	0.0	0.0	0.0028898083451139632	5.127079321976387E-4
24	3.2626868412577003E-4	0.0	0.0	0.004148273269599077	5.127079321976387E-4
25	3.728784961437372E-4	0.0	0.0	0.005639787254174025	5.127079321976387E-4
26	3.728784961437372E-4	0.0	0.0	0.008389766163234088	5.127079321976387E-4
27	3.728784961437372E-4	0.0	0.0	0.018923583679294663	5.127079321976387E-4
28	3.728784961437372E-4	0.0	0.0	0.05224959927214118	5.127079321976387E-4
29	4.1948830816170436E-4	0.0	0.0	0.09997804677853954	5.127079321976387E-4
30	4.660981201796715E-4	0.0	0.0	0.16355383037104673	5.127079321976387E-4
31	4.660981201796715E-4	0.0	0.0	0.3535820339682988	5.127079321976387E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACAGGC	3155	0.0	20.992075	7
TGCATCA	3140	0.0	20.62102	14
ACAGGCT	3245	0.0	20.466871	8
CCAGGAC	3430	0.0	20.279882	3
CAGGACA	3385	0.0	20.057608	4
TCTAGCG	130	3.274181E-11	19.923079	28
GCATCAG	3320	0.0	19.837349	15
TCCAGGA	3600	0.0	19.579166	2
GCTGCAT	3295	0.0	19.538694	12
CTGCATC	3435	0.0	19.065502	13
GGTATCA	780	0.0	18.97436	1
GGACAGG	3595	0.0	18.834492	6
GGGTACG	50	0.007036823	18.499998	1
CTTATAC	810	0.0	18.04321	37
GGCCATC	3575	0.0	18.008392	26
TAACGCC	165	3.6379788E-12	17.939392	4
CATCAGA	3705	0.0	17.62618	16
ATCAGAA	3730	0.0	17.458445	17
AAGAGGC	3760	0.0	17.269945	22
TATCCCG	75	2.0683189E-4	17.266666	10
>>END_MODULE