FastQCFastQC Report
Fri 10 Feb 2017
ERR1630311.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630311.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2327091
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56090.24103053984566997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47660.2048050548947162No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT42430.18233064370924904No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG41480.17824829368512018No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37010.15903976251895607No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA33270.1429681950555436No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG32800.14094850609623774No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC32620.14017500819692913No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA27820.11954839754869923No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27030.11615360121284471No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC25570.10987967380734144No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC25280.10863348274734422No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT25250.10850456643079279No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC23980.10304710903011528No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT23620.10150011323149803No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC23530.10111336428184374No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC23410.10059769901563799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18950.022.4538251
TAACGCT1054.8024776E-717.61904732
ACGGACC2950.017.5593228
CGGACCA3100.016.7096799
GTACGGA904.449106E-516.4444456
CTTAACG1151.2434575E-616.08695630
TACGTTA1151.2434575E-616.08695619
GTGCGAC700.002593564815.85714211
TCTAGCG2250.015.62222228
GACGGAC3200.015.6093757
CGGCCTT8850.015.05084724
TCGCCAT9100.015.04395613
GCGAAAG3200.015.0312518
CTTATAC11450.015.026237
ATCTCGC9000.015.00555611
CGCAATA2350.014.95744736
GTATCAA28750.014.9286972
ACGTTAA1252.9617167E-614.79999920
CGCTAAG1252.9617167E-614.79999935
CGAAAGC3550.014.59154919
Produced by FastQC (version 0.11.5)