##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630311.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2327091
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73571768358006	31.0	31.0	34.0	30.0	34.0
2	31.95817095248961	33.0	31.0	34.0	30.0	34.0
3	32.07150859162792	33.0	31.0	34.0	30.0	34.0
4	35.74017260175902	37.0	35.0	37.0	33.0	37.0
5	35.64990496718865	37.0	35.0	37.0	33.0	37.0
6	35.71006247714421	37.0	35.0	37.0	33.0	37.0
7	35.70041609889772	37.0	35.0	37.0	33.0	37.0
8	35.703737412933144	37.0	35.0	37.0	33.0	37.0
9	37.42890071767714	39.0	37.0	39.0	35.0	39.0
10	37.31450811334838	39.0	37.0	39.0	34.0	39.0
11	37.37979993047114	39.0	37.0	39.0	34.0	39.0
12	37.312299776845855	39.0	37.0	39.0	34.0	39.0
13	37.31528161124769	39.0	37.0	39.0	34.0	39.0
14	38.538726246631526	40.0	38.0	41.0	34.0	41.0
15	38.54809588451848	40.0	38.0	41.0	34.0	41.0
16	38.49359737113847	40.0	38.0	41.0	34.0	41.0
17	38.470882745883166	40.0	38.0	41.0	34.0	41.0
18	38.41709370196524	40.0	38.0	41.0	34.0	41.0
19	38.471789457309576	40.0	38.0	41.0	34.0	41.0
20	38.441182145433935	40.0	38.0	41.0	34.0	41.0
21	38.38273879276745	40.0	38.0	41.0	34.0	41.0
22	38.3573654833438	40.0	38.0	41.0	34.0	41.0
23	38.30251245009327	40.0	38.0	41.0	34.0	41.0
24	38.27268035500116	40.0	38.0	41.0	34.0	41.0
25	38.25039931829052	40.0	38.0	41.0	34.0	41.0
26	38.091200129260095	40.0	38.0	41.0	33.0	41.0
27	37.97700648577989	40.0	37.0	41.0	33.0	41.0
28	37.92334335013113	40.0	37.0	41.0	33.0	41.0
29	37.87715521223708	40.0	37.0	41.0	33.0	41.0
30	37.82149430340283	40.0	37.0	41.0	33.0	41.0
31	37.76500446265316	40.0	37.0	41.0	33.0	41.0
32	37.67872378003267	40.0	37.0	41.0	33.0	41.0
33	37.59767752958522	40.0	37.0	41.0	32.0	41.0
34	37.54029129071446	40.0	37.0	41.0	32.0	41.0
35	37.429813015477265	40.0	37.0	41.0	32.0	41.0
36	37.35103311387479	40.0	37.0	41.0	31.0	41.0
37	37.29393865560049	40.0	36.0	41.0	31.0	41.0
38	37.18888775729011	39.0	36.0	41.0	31.0	41.0
39	37.1208942838935	39.0	36.0	41.0	31.0	41.0
40	37.0394698789175	39.0	36.0	41.0	31.0	41.0
41	36.935408198476125	39.0	36.0	41.0	30.0	41.0
42	36.91413571708197	39.0	36.0	41.0	30.0	41.0
43	35.72590844105366	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	10.0
10	8.0
11	3.0
12	3.0
13	1.0
14	4.0
15	13.0
16	20.0
17	46.0
18	87.0
19	200.0
20	363.0
21	716.0
22	1318.0
23	2223.0
24	3847.0
25	6054.0
26	9262.0
27	13722.0
28	19631.0
29	26992.0
30	36975.0
31	47831.0
32	61736.0
33	80120.0
34	106305.0
35	141783.0
36	200425.0
37	305498.0
38	588397.0
39	673496.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.210635939892335	20.557382586241793	13.238889239827751	24.99309223403812
2	17.87373162459053	22.182114923739555	36.13193467724296	23.812218774426956
3	19.599061661103928	24.199741221980574	31.08825568059006	25.112941436325436
4	14.988025822797649	16.462097958352295	35.063390301453616	33.48648591739644
5	14.046249158283883	37.20374493305161	34.731903479494356	14.018102429170154
6	33.11890252680278	35.720004073755604	16.38883051844556	14.77226288099606
7	27.139248099880923	31.61234347947717	22.43943189157622	18.80897652906569
8	25.012085904676695	35.47609440284029	21.099303808918517	18.412515883564502
9	25.57016463902787	15.423891889058055	20.915512113621684	38.09043135829239
10	16.27521227145823	27.935435270902598	34.236649963409256	21.55270249422992
11	33.83761099157704	22.94663165299509	22.72670041695834	20.48905693846953
12	19.859515592643348	26.795943948904448	30.28957612744839	23.054964331003816
13	28.485435249416547	21.10635123422333	26.36076543633231	24.047448080027813
14	21.800178849903162	22.432298522060375	25.792330424551512	29.97519220348495
15	24.23643940009222	29.175008626650182	23.637107444444588	22.95144452881301
16	23.975126026442457	27.956534574711515	24.781927307526868	23.28641209131916
17	22.707362969475625	27.896201738565445	26.235372832433285	23.161062459525645
18	21.730907815809523	26.675879886089543	27.969727011105284	23.623485286995653
19	23.171805485904933	27.00689401488812	27.94905742835153	21.872243070855415
20	23.466465213436003	26.140748256084528	28.117336193556675	22.275450336922795
21	22.946545708784058	27.307354976664	26.767453442946582	22.978645871605366
22	22.990076451672927	26.643049197474443	27.101733451764453	23.265140899088173
23	22.488935757131973	27.16584783319604	27.19476806020908	23.150448349462913
24	23.9283723756398	27.01192175123362	26.897744866874568	22.161961006252014
25	23.620520211714968	26.205550191204384	27.21457820085248	22.95935139622817
26	23.34833489536937	26.871445938298073	26.881630327305633	22.898588839026925
27	23.578020799358512	26.34065449095029	26.626676825272412	23.454647884418787
28	22.58459166401314	26.842998404445723	27.403139799861716	23.169270131679422
29	22.763398595069983	27.253811733189636	27.233442955174507	22.749346716565878
30	22.409523306136286	27.899811395428888	27.06116778415627	22.629497514278558
31	23.112675868713342	26.861992075084302	27.36480868174042	22.660523374461935
32	22.253835367847667	26.928040201264153	27.323082767283275	23.495041663604905
33	22.533068109498082	26.66376175233371	27.55616346760827	23.247006670559937
34	22.833615015484998	26.600549785117988	27.311351382477095	23.254483816919922
35	23.27003971911713	26.512371024596803	27.289177776030243	22.92841148025582
36	22.702893870501843	26.908917614309026	27.423250745243738	22.964937769945397
37	23.629931102823225	25.492213239619765	27.23271242938072	23.645143228176295
38	22.66709810660606	26.254495419388412	28.144494564243512	22.933911909762017
39	22.66013662551228	25.888845773543018	28.3675197918775	23.0834978090672
40	22.55356580382976	25.27009042620164	29.015195366231914	23.161148403736682
41	21.794635448291448	26.14702218349003	29.14570165068749	22.91264071753103
42	22.625157331621324	25.111308496315786	28.706311871774677	23.557222300288213
43	21.657984152746927	25.50076468861768	28.842146697314377	23.99910446132102
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	768.0
1	664.5
2	561.0
3	1009.0
4	1457.0
5	1457.0
6	1912.5
7	2368.0
8	2188.5
9	2009.0
10	3116.0
11	4223.0
12	4223.0
13	6558.0
14	8893.0
15	13025.5
16	17158.0
17	17750.0
18	18342.0
19	18342.0
20	21865.5
21	25389.0
22	29043.0
23	32697.0
24	36677.0
25	40657.0
26	40657.0
27	45315.0
28	49973.0
29	64626.5
30	79280.0
31	82904.0
32	86528.0
33	86528.0
34	93215.5
35	99903.0
36	105932.0
37	111961.0
38	127265.0
39	142569.0
40	142569.0
41	152442.5
42	162316.0
43	152140.0
44	141964.0
45	149692.5
46	157421.0
47	157421.0
48	163595.5
49	169770.0
50	173988.5
51	178207.0
52	183445.0
53	188683.0
54	188683.0
55	162700.5
56	136718.0
57	126762.0
58	116806.0
59	104891.0
60	92976.0
61	92976.0
62	84915.5
63	76855.0
64	67240.0
65	57625.0
66	49737.0
67	41849.0
68	41849.0
69	35447.0
70	29045.0
71	25060.0
72	21075.0
73	17118.0
74	13161.0
75	13161.0
76	10239.0
77	7317.0
78	5997.5
79	4678.0
80	3702.0
81	2726.0
82	2726.0
83	1994.0
84	1262.0
85	1036.0
86	810.0
87	707.5
88	605.0
89	605.0
90	492.5
91	380.0
92	224.5
93	69.0
94	47.0
95	25.0
96	25.0
97	17.0
98	9.0
99	6.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2327091.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.76863365878109
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.20439169337405	42.74941914341417
2	9.608153458611035	9.755856461551485
3	2.555442069933093	3.892089068540113
4	1.0958551813692505	2.225402809840504
5	0.554331342516695	1.4071322426905197
6	0.3661395283593784	1.1153042149965706
7	0.24833953502967665	0.8825501213839583
8	0.17011673836367933	0.6909275495369879
9	0.13666990967714643	0.6244690118919973
>10	0.8202789481484226	7.97189272402368
>50	0.10233242998583449	3.65571109755007
>100	0.11244738926168675	12.224285429795195
>500	0.01846972435453645	6.350649437769099
>1k	0.006947327509504534	6.213050957959938
>5k	8.472350621346993E-5	0.2412597290558222
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5609	0.24103053984566997	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4766	0.2048050548947162	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	4243	0.18233064370924904	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	4148	0.17824829368512018	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3701	0.15903976251895607	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	3327	0.1429681950555436	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	3280	0.14094850609623774	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	3262	0.14017500819692913	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2782	0.11954839754869923	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2703	0.11615360121284471	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2557	0.10987967380734144	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	2528	0.10863348274734422	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	2525	0.10850456643079279	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	2398	0.10304710903011528	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	2362	0.10150011323149803	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	2353	0.10111336428184374	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	2341	0.10059769901563799	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.2972105517145654E-5	0.0
2	0.0	0.0	0.0	4.2972105517145654E-5	0.0
3	0.0	0.0	0.0	4.2972105517145654E-5	0.0
4	0.0	0.0	0.0	8.594421103429131E-5	0.0
5	0.0	0.0	0.0	8.594421103429131E-5	0.0
6	0.0	0.0	0.0	8.594421103429131E-5	0.0
7	0.0	0.0	0.0	8.594421103429131E-5	0.0
8	0.0	0.0	0.0	8.594421103429131E-5	0.0
9	0.0	0.0	0.0	1.7188842206858262E-4	0.0
10	0.0	0.0	0.0	2.1486052758572826E-4	4.2972105517145654E-5
11	0.0	0.0	0.0	3.4377684413716523E-4	8.594421103429131E-5
12	0.0	0.0	0.0	3.4377684413716523E-4	8.594421103429131E-5
13	0.0	0.0	0.0	3.867489496543109E-4	8.594421103429131E-5
14	0.0	0.0	0.0	4.2972105517145653E-4	8.594421103429131E-5
15	0.0	0.0	0.0	5.156652662057479E-4	8.594421103429131E-5
16	0.0	0.0	0.0	7.305257937914761E-4	8.594421103429131E-5
17	0.0	0.0	0.0	0.0011602468489629327	8.594421103429131E-5
18	0.0	0.0	0.0	0.001246191059997224	8.594421103429131E-5
19	0.0	0.0	0.0	0.001375107376548661	8.594421103429131E-5
20	0.0	0.0	0.0	0.0016329400096515348	8.594421103429131E-5
21	0.0	0.0	0.0	0.0018478005372372633	1.2891631655143697E-4
22	0.0	0.0	0.0	0.002363465803443011	1.2891631655143697E-4
23	0.0	0.0	0.0	0.003394796335854507	1.2891631655143697E-4
24	0.0	0.0	0.0	0.0047699037124031675	1.2891631655143697E-4
25	0.0	0.0	0.0	0.006445815827571848	1.2891631655143697E-4
26	0.0	0.0	0.0	0.010012500585494938	1.2891631655143697E-4
27	0.0	0.0	0.0	0.02513868172753021	1.2891631655143697E-4
28	0.0	0.0	0.0	0.06153605510055258	1.2891631655143697E-4
29	0.0	0.0	0.0	0.11039533907354719	1.2891631655143697E-4
30	0.0	0.0	0.0	0.17188842206858262	1.2891631655143697E-4
31	0.0	0.0	0.0	0.35176965576335434	1.2891631655143697E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1895	0.0	22.453825	1
TAACGCT	105	4.8024776E-7	17.619047	32
ACGGACC	295	0.0	17.559322	8
CGGACCA	310	0.0	16.709679	9
GTACGGA	90	4.449106E-5	16.444445	6
CTTAACG	115	1.2434575E-6	16.086956	30
TACGTTA	115	1.2434575E-6	16.086956	19
GTGCGAC	70	0.0025935648	15.857142	11
TCTAGCG	225	0.0	15.622222	28
GACGGAC	320	0.0	15.609375	7
CGGCCTT	885	0.0	15.050847	24
TCGCCAT	910	0.0	15.043956	13
GCGAAAG	320	0.0	15.03125	18
CTTATAC	1145	0.0	15.0262	37
ATCTCGC	900	0.0	15.005556	11
CGCAATA	235	0.0	14.957447	36
GTATCAA	2875	0.0	14.928697	2
ACGTTAA	125	2.9617167E-6	14.799999	20
CGCTAAG	125	2.9617167E-6	14.799999	35
CGAAAGC	355	0.0	14.591549	19
>>END_MODULE