FastQCFastQC Report
Fri 10 Feb 2017
ERR1630310.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630310.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1771301
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT139900.7898149439310429No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT93630.5285945189439852No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT91550.5168517377904716No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40310.2275728405279509No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA20100.11347591403155083No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGT400.001931597823.1254
CGCACTA450.00382661420.55555529
GTGCGTA852.7244636E-517.41176415
TGCGTAC852.7244636E-517.41176416
GGTATCA38300.017.3890341
TCGTTTA1502.5102054E-1017.26666630
CAGTCGG2400.016.95833410
TACGGCG1107.813469E-716.81818219
CGTTATT2100.016.7380962
CTTATAC10400.016.72115337
CCGGATA904.4481436E-516.4444454
GCAGTCG2600.015.6538469
GCGTTAT2250.015.6222211
TACCGTC2250.015.6222217
TATACTG1557.215931E-915.5161295
GACGGAC2750.015.4727287
AAGACGG3000.015.4166675
ACGGACC3000.015.4166678
TACGACG1802.0190782E-1015.4166665
GCTTAGG5500.015.1363641
Produced by FastQC (version 0.11.5)