FastQCFastQC Report
Fri 10 Feb 2017
ERR1630309.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630309.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2922750
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT115580.3954494910615003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT101810.3483363270892139No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT82290.2815499101873236No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60540.207133692584039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42050.020.2818091
CGAACGA3400.016.32352816
CGGACCA5050.015.0198019
ACGGACC5100.014.8725498
GACGGAC5550.014.3333337
CGCAAGA5550.014.3333332
AAGACGG5850.014.230775
ATAACGA4550.013.82417512
ATCGTAC1501.3073568E-613.56666710
GTATCAA63650.013.3409262
CGAATTA2651.8189894E-1213.26415215
TCTTATA27450.013.20947237
TCTAGCG3250.013.09230728
CTAGCGG3400.013.05882329
CGAAAGC6000.012.9519
CGATACG1000.001833657212.94999926
AGACGGA6350.012.8188976
TTTCGGA4050.012.79012330
TAGGACC7000.012.6857144
GCGAAAG6000.012.64166618
Produced by FastQC (version 0.11.5)