##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630309.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2922750
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90777452741425	33.0	31.0	34.0	30.0	34.0
2	32.1153054486357	33.0	31.0	34.0	30.0	34.0
3	32.243560003421436	34.0	31.0	34.0	30.0	34.0
4	35.867103583953465	37.0	35.0	37.0	35.0	37.0
5	35.80051903173381	37.0	35.0	37.0	35.0	37.0
6	35.863851851851855	37.0	35.0	37.0	35.0	37.0
7	35.84114960225815	37.0	35.0	37.0	35.0	37.0
8	35.85762073389787	37.0	35.0	37.0	35.0	37.0
9	37.60066615345137	39.0	37.0	39.0	35.0	39.0
10	37.492288426995124	39.0	37.0	39.0	35.0	39.0
11	37.56283534342657	39.0	37.0	39.0	35.0	39.0
12	37.49193191343769	39.0	37.0	39.0	35.0	39.0
13	37.50675972970661	39.0	37.0	39.0	35.0	39.0
14	38.7509516722265	40.0	38.0	41.0	35.0	41.0
15	38.75710854503464	40.0	38.0	41.0	35.0	41.0
16	38.71300898126764	40.0	38.0	41.0	35.0	41.0
17	38.69875938756308	40.0	38.0	41.0	35.0	41.0
18	38.66730510649217	40.0	38.0	41.0	34.0	41.0
19	38.70227491232572	40.0	38.0	41.0	34.0	41.0
20	38.68192934736122	40.0	38.0	41.0	34.0	41.0
21	38.634531862116155	40.0	38.0	41.0	34.0	41.0
22	38.59524865280985	40.0	38.0	41.0	34.0	41.0
23	38.53609648447524	40.0	38.0	41.0	34.0	41.0
24	38.50328389359336	40.0	38.0	41.0	34.0	41.0
25	38.48104148490292	40.0	38.0	41.0	34.0	41.0
26	38.32389667265418	40.0	38.0	41.0	34.0	41.0
27	38.21243110084681	40.0	38.0	41.0	34.0	41.0
28	38.14670088101959	40.0	38.0	41.0	33.0	41.0
29	38.08290890428535	40.0	38.0	41.0	33.0	41.0
30	38.0124146779574	40.0	37.0	41.0	33.0	41.0
31	37.954727226071334	40.0	37.0	41.0	33.0	41.0
32	37.86696022581473	40.0	37.0	41.0	33.0	41.0
33	37.7691478915405	40.0	37.0	41.0	33.0	41.0
34	37.70494534257121	40.0	37.0	41.0	33.0	41.0
35	37.5831531947652	40.0	37.0	41.0	33.0	41.0
36	37.4984925156103	40.0	37.0	41.0	32.0	41.0
37	37.4335297237191	40.0	37.0	41.0	32.0	41.0
38	37.3339666410059	40.0	36.0	41.0	32.0	41.0
39	37.25210401163288	40.0	36.0	41.0	31.0	41.0
40	37.144612436917285	40.0	36.0	41.0	31.0	41.0
41	37.02294072363357	39.0	36.0	41.0	31.0	41.0
42	36.9683151141904	39.0	36.0	41.0	31.0	41.0
43	35.82467162774784	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	2.0
10	8.0
11	9.0
12	8.0
13	8.0
14	4.0
15	9.0
16	24.0
17	49.0
18	81.0
19	173.0
20	339.0
21	686.0
22	1315.0
23	2335.0
24	4077.0
25	6744.0
26	10028.0
27	14815.0
28	21868.0
29	30192.0
30	41328.0
31	54949.0
32	71374.0
33	94272.0
34	125874.0
35	170442.0
36	240337.0
37	369611.0
38	677958.0
39	983827.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.90464459840903	20.375742023778976	12.832811564451289	24.886801813360705
2	18.197416816354462	21.62678983833718	35.216354460696266	24.959438884612094
3	19.499683517235482	24.486699170301943	29.96883072448892	26.044786587973658
4	14.044683944914894	16.41081173552305	35.93766144897785	33.60684287058421
5	13.91624326404927	37.389718586947225	34.50428534770336	14.189752801300145
6	32.54065520485844	36.27552818407322	16.56460525190317	14.619211359165169
7	28.004995295526474	31.31246257805149	22.545753143443676	18.13678898297836
8	26.645864340090668	33.925378496279194	20.64788298691301	18.780874176717134
9	26.577230348131042	14.512223077581046	21.046377555384485	37.86416901890343
10	17.1681464374305	27.031460097510905	33.68601488324352	22.11437858181507
11	34.341493456505	22.920674022752543	22.292909075357112	20.44492344538534
12	20.55670173637841	26.946334787443334	30.139491916859125	22.357471559319134
13	29.230177059276368	20.830245487982207	25.95079976049953	23.988777692241893
14	22.135283551449835	21.73873920109486	26.426071336925837	29.699905910529466
15	24.634539389273797	28.528406466512703	23.36641861260799	23.470635531605506
16	23.944196390385766	26.820118039517578	25.660935762552388	23.574749807544265
17	22.435685570096656	27.34914036438286	26.723291420751003	23.49188264476948
18	22.609802412111883	25.99257548541613	27.865537593020274	23.532084509451714
19	22.94332392438628	26.26362158925669	28.19984603541186	22.59320845094517
20	23.24105722350526	25.924557351809085	27.959421777435633	22.87496364725002
21	23.73839705756565	26.016525532460864	27.567530579077925	22.67754683089556
22	23.637362073389788	25.8176374989308	27.865640236079038	22.679360191600377
23	23.282046018304676	26.017038747754683	27.797519459413223	22.903395774527414
24	22.70840817723035	26.66464801984432	27.44651441279617	23.18042939012916
25	23.35906252672996	26.03031391668805	27.733949191685912	22.876674364896076
26	22.831100846805235	26.800786930117184	27.610538020699682	22.7575742023779
27	23.167427936019163	26.337250876742797	27.41144470105209	23.083876486185957
28	22.901685056881362	26.36133778119921	28.033119493627577	22.70385766829185
29	22.611581558463776	26.39189119835771	28.21907450175349	22.777452741425027
30	22.47455307501497	26.941544778034388	27.784175861774013	22.79972628517663
31	22.99221623471046	26.6338893165683	27.57321016166282	22.80068428705842
32	22.5420579933282	26.4554272517321	27.669284064665128	23.33323069027457
33	22.218116499871694	26.476537507484387	27.99387563082713	23.311470361816784
34	23.26350183902147	25.94730989650158	27.834676246685486	22.954512017791465
35	22.889333675476863	26.12057137969378	28.300983662646477	22.689111282182875
36	22.587802583183645	26.37533145154392	27.56995979813532	23.466906167137115
37	22.90096655547002	25.51312975793345	28.141134205799332	23.444769480797195
38	22.23351295868617	25.777538277307332	28.487862458301255	23.501086305705243
39	22.419604824223764	25.628124198101105	28.607646907877854	23.34462406979728
40	22.198990676588828	25.365597468137885	29.27325292960397	23.162158925669317
41	21.696210760413994	25.503070738174667	29.390847660593618	23.409870840817725
42	21.710957146522965	25.737199555213415	29.2664784877256	23.28536481053802
43	21.471456676075615	24.988110512359935	29.585801043537764	23.954631768026687
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2480.0
1	2034.5
2	1589.0
3	2574.5
4	3560.0
5	3560.0
6	4377.5
7	5195.0
8	4508.5
9	3822.0
10	5427.5
11	7033.0
12	7033.0
13	12046.0
14	17059.0
15	21695.0
16	26331.0
17	26193.5
18	26056.0
19	26056.0
20	32304.0
21	38552.0
22	37944.5
23	37337.0
24	43503.0
25	49669.0
26	49669.0
27	56815.5
28	63962.0
29	72925.5
30	81889.0
31	91958.5
32	102028.0
33	102028.0
34	114265.5
35	126503.0
36	137305.0
37	148107.0
38	155147.0
39	162187.0
40	162187.0
41	173150.5
42	184114.0
43	189104.5
44	194095.0
45	198514.0
46	202933.0
47	202933.0
48	206798.0
49	210663.0
50	204203.5
51	197744.0
52	194841.0
53	191938.0
54	191938.0
55	184120.5
56	176303.0
57	165131.0
58	153959.0
59	144527.5
60	135096.0
61	135096.0
62	123646.0
63	112196.0
64	99965.0
65	87734.0
66	75995.0
67	64256.0
68	64256.0
69	54152.5
70	44049.0
71	36419.5
72	28790.0
73	22114.0
74	15438.0
75	15438.0
76	11935.5
77	8433.0
78	6638.0
79	4843.0
80	3928.0
81	3013.0
82	3013.0
83	2235.0
84	1457.0
85	1207.5
86	958.0
87	862.5
88	767.0
89	767.0
90	621.5
91	476.0
92	278.5
93	81.0
94	60.5
95	40.0
96	40.0
97	23.0
98	6.0
99	7.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2922750.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.1341234315697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.98567887141851	41.699832550404295
2	11.560254272425528	12.05367446277927
3	3.497439949205777	5.470078980191905
4	1.4428464140453905	3.008861321705605
5	0.8020729376775609	2.090768476700184
6	0.4853923928254164	1.5183304152183137
7	0.3382960670318275	1.2345738240536313
8	0.2497234511533168	1.041529058094767
9	0.19160783617280908	0.8990375923340294
>10	1.2010761421513523	12.28926707875436
>50	0.13745276177288923	5.01368120540127
>100	0.1017849948984079	9.716321925614627
>500	0.004731149112755226	1.6804010143361057
>1k	0.0013799184911947057	1.0496168693941188
>5k	1.3142080868521006E-4	0.48930049105898354
>10k+	1.3142080868521006E-4	0.7447247339586391
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11558	0.3954494910615003	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10181	0.3483363270892139	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8229	0.2815499101873236	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6054	0.207133692584039	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.4214352921050384E-5	0.0
5	0.0	0.0	0.0	3.4214352921050384E-5	0.0
6	0.0	0.0	0.0	3.4214352921050384E-5	0.0
7	0.0	0.0	0.0	3.4214352921050384E-5	0.0
8	0.0	0.0	0.0	3.4214352921050384E-5	0.0
9	0.0	0.0	0.0	1.0264305876315114E-4	0.0
10	0.0	0.0	0.0	1.3685741168420153E-4	0.0
11	0.0	0.0	0.0	1.3685741168420153E-4	0.0
12	0.0	0.0	0.0	2.0528611752630227E-4	0.0
13	0.0	0.0	0.0	2.0528611752630227E-4	0.0
14	0.0	0.0	0.0	3.763578821315542E-4	0.0
15	0.0	0.0	0.0	8.211444701052091E-4	0.0
16	0.0	0.0	0.0	0.001163287999315713	0.0
17	0.0	0.0	0.0	0.002052861175263023	0.0
18	0.0	0.0	0.0	0.002326575998631426	0.0
19	0.0	0.0	0.0	0.002805576939526131	0.0
20	0.0	0.0	0.0	0.0030108630570524334	0.0
21	0.0	0.0	0.0	0.0039688649388418444	0.0
22	0.0	0.0	0.0	0.0056453682319733125	0.0
23	0.0	0.0	0.0	0.0076640150543152855	0.0
24	0.0	0.0	0.0	0.011838166110683432	0.0
25	0.0	0.0	0.0	0.013617312462578052	0.0
26	0.0	0.0	0.0	0.01905739457702506	0.0
27	0.0	0.0	0.0	0.03855957574202378	0.0
28	0.0	0.0	0.0	0.07992472842357369	0.0
29	0.0	0.0	0.0	0.13596783850825422	0.0
30	0.0	0.0	0.0	0.21763749893080148	0.0
31	0.0	0.0	0.0	0.4391754340946027	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4205	0.0	20.281809	1
CGAACGA	340	0.0	16.323528	16
CGGACCA	505	0.0	15.019801	9
ACGGACC	510	0.0	14.872549	8
GACGGAC	555	0.0	14.333333	7
CGCAAGA	555	0.0	14.333333	2
AAGACGG	585	0.0	14.23077	5
ATAACGA	455	0.0	13.824175	12
ATCGTAC	150	1.3073568E-6	13.566667	10
GTATCAA	6365	0.0	13.340926	2
CGAATTA	265	1.8189894E-12	13.264152	15
TCTTATA	2745	0.0	13.209472	37
TCTAGCG	325	0.0	13.092307	28
CTAGCGG	340	0.0	13.058823	29
CGAAAGC	600	0.0	12.95	19
CGATACG	100	0.0018336572	12.949999	26
AGACGGA	635	0.0	12.818897	6
TTTCGGA	405	0.0	12.790123	30
TAGGACC	700	0.0	12.685714	4
GCGAAAG	600	0.0	12.641666	18
>>END_MODULE