FastQCFastQC Report
Fri 10 Feb 2017
ERR1630308.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630308.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314899
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT87872.7904185151429504No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT79632.528747312630399No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56571.7964490201620202No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36671.1645003636086493No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14000.44458699456016054No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA12230.38837849596219737No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10870.3451900450620675No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA9900.31438651758182784No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9660.30676502624651075No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8710.2765966230442142No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8540.27119806668169794No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5640.1791050463799504No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5320.16894305793286102No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA4950.15719325879091392No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA4590.14576102178793837No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA4550.14449077323205217No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA4510.143220524676166No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4420.1403624654254221No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA3880.12321410992095878No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA3700.117497991419471No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3520.11178187291798322No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA3380.10733600297238163No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA3360.10670087869443852No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT3340.10606575441649545No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3300.10479550586060928No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA3290.10447794372163773No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAA200.001839907537.017
ACGTTTA250.00549035929.626
TGGACGT250.00549035929.626
AGTGGAC250.00549035929.624
TTTCAAT250.00549035929.631
TCACGTT250.00549035929.624
GTATACT405.9266495E-527.754
CGAGGAG358.85504E-426.4285731
ACTGTGC856.91216E-1126.1176478
CTGTGCG809.713403E-1025.43759
GTCGTCA601.3322588E-624.66666824
CATAATC601.3322588E-624.66666837
AGGTCGT601.3322588E-624.66666822
AGTCCTA451.3202333E-424.66666629
CCGTATA451.3202333E-424.6666662
CTGATCA451.3202333E-424.6666664
TACTGTG1150.024.1304367
GCATAAT551.8969758E-523.54545436
CGGGATA551.8969758E-523.54545424
GTATTAG400.001928091523.1251
Produced by FastQC (version 0.11.5)