##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630308.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	314899
Sequences flagged as poor quality	0
Sequence length	43
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.776306053687055	31.0	31.0	34.0	30.0	34.0
2	31.885468038958525	33.0	31.0	34.0	30.0	34.0
3	31.924048663222177	33.0	31.0	34.0	30.0	34.0
4	35.622593275939266	37.0	35.0	37.0	33.0	37.0
5	35.60013845709259	37.0	35.0	37.0	33.0	37.0
6	35.72128523748884	37.0	35.0	37.0	33.0	37.0
7	35.68797296911073	37.0	35.0	37.0	33.0	37.0
8	35.772361931921026	37.0	35.0	37.0	35.0	37.0
9	37.41543161458118	39.0	37.0	39.0	34.0	39.0
10	37.33431671742368	39.0	37.0	39.0	34.0	39.0
11	37.432500579550904	39.0	37.0	39.0	34.0	39.0
12	37.40047126221423	39.0	37.0	39.0	34.0	39.0
13	37.41793717985767	39.0	37.0	39.0	34.0	39.0
14	38.47164964004332	40.0	38.0	41.0	34.0	41.0
15	38.46451084315923	40.0	38.0	41.0	34.0	41.0
16	38.327482780193016	40.0	38.0	41.0	34.0	41.0
17	38.47842324046758	40.0	38.0	41.0	34.0	41.0
18	38.53041451386	40.0	38.0	41.0	34.0	41.0
19	38.58993836118883	40.0	38.0	41.0	34.0	41.0
20	38.628563444151936	40.0	38.0	41.0	34.0	41.0
21	38.590999018732994	40.0	38.0	41.0	34.0	41.0
22	38.52467616600878	40.0	38.0	41.0	34.0	41.0
23	38.41729887995834	40.0	38.0	41.0	34.0	41.0
24	38.34240502510329	40.0	38.0	41.0	34.0	41.0
25	38.293097151785176	40.0	37.0	41.0	34.0	41.0
26	37.98761190095872	40.0	37.0	41.0	33.0	41.0
27	37.75221261420329	40.0	36.0	41.0	33.0	41.0
28	37.61148812793943	40.0	36.0	41.0	33.0	41.0
29	37.4273751266279	39.0	35.0	41.0	33.0	41.0
30	37.16153115760927	39.0	35.0	41.0	33.0	41.0
31	36.87978367667093	39.0	35.0	41.0	32.0	41.0
32	36.5834315129613	38.0	35.0	40.0	31.0	41.0
33	36.33936913105472	38.0	35.0	40.0	31.0	41.0
34	36.1304577023109	38.0	35.0	40.0	30.0	41.0
35	35.8586753212935	38.0	35.0	40.0	30.0	41.0
36	35.54784549966815	38.0	35.0	40.0	29.0	41.0
37	35.271118676146955	38.0	35.0	40.0	27.0	41.0
38	34.932197307708186	38.0	35.0	40.0	25.0	41.0
39	34.578874496267055	37.0	34.0	40.0	23.0	41.0
40	34.1655038599678	37.0	33.0	40.0	21.0	41.0
41	33.73919256650545	37.0	33.0	40.0	18.0	41.0
42	33.33876893861206	37.0	33.0	40.0	15.0	41.0
43	32.16792050784537	35.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	7.0
11	9.0
12	2.0
13	5.0
14	5.0
15	4.0
16	4.0
17	6.0
18	9.0
19	47.0
20	84.0
21	139.0
22	321.0
23	545.0
24	911.0
25	1464.0
26	2141.0
27	3236.0
28	4363.0
29	5762.0
30	7196.0
31	8998.0
32	10928.0
33	13785.0
34	18115.0
35	22731.0
36	30973.0
37	51755.0
38	61719.0
39	69632.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.76737620633917	20.39987424539297	14.137548864874134	18.695200683393722
2	17.7625206812343	26.648544453936022	33.152852184351175	22.436082680478503
3	22.276031362436846	28.094087310534487	30.97628128384022	18.65360004318845
4	14.27473570890984	18.077224760955097	32.663806490335	34.98423303980006
5	12.552596229267163	41.71972600738649	30.640300540808322	15.08737722253802
6	28.797487448356456	33.96041270375581	18.67582939291646	18.566270454971274
7	24.018812381112674	35.268451154179594	21.983239070305082	18.72949739440265
8	30.519626927999138	31.333856252322175	20.58945884235898	17.55705797731971
9	28.279543599693866	12.756153560347922	22.784130784791316	36.18017205516689
10	20.57326317327143	30.251922044846125	27.778430544396777	21.39638423748567
11	30.196666232665077	26.743177971349546	19.459255189759254	23.600900606226123
12	19.504666575632186	32.205881885938034	28.807331874664577	19.482119663765207
13	31.006449687042515	21.269676944036025	27.77303198803426	19.950841380887205
14	22.127729843537132	24.40052207215647	30.499302951104955	22.97244513320144
15	27.198244516495766	27.19887964077371	26.76318438610475	18.839691456625776
16	19.383992962823	27.477381636651753	30.682536305291535	22.45608909523371
17	18.48052867744896	30.31733984547426	30.19698379480405	21.005147682272728
18	17.370649001743416	25.90513148660364	33.98359473990073	22.740624771752213
19	18.506251210705653	28.74604238184307	35.493285148571445	17.25442125887983
20	19.592631288127304	26.35956290747192	36.24971816360166	17.798087640799114
21	21.037221458308856	26.681570916389063	34.23002295974265	18.051184665559433
22	21.169962432398957	27.6129806699926	32.972159327276366	18.244897570332075
23	19.48688309584978	28.28780021530713	33.5755273913223	18.64978929752079
24	18.370017053086862	29.357031937224313	33.8556171978952	18.417333811793625
25	18.493866287285766	29.389105713260445	33.725734283055836	18.391293716397954
26	19.05499858684848	29.129657445720692	33.653012553231356	18.16233141419947
27	18.44623196644003	29.592980606480175	33.87181286698275	18.088974560097046
28	17.637401198479512	30.91816741240842	34.06107990180979	17.383351487302278
29	17.903835833076638	30.737792117472583	33.85879281928491	17.499579230165864
30	17.158835055049394	32.2519283960889	34.57902375047237	16.010212798389325
31	17.934004236278934	30.976598845979186	33.492961235189696	17.596435682552183
32	16.309038771161546	31.81909120067069	33.64380325120118	18.228066776966585
33	16.864454952222776	31.59457476841781	34.22335415482424	17.317616124535167
34	16.23695216561501	31.873711888573798	33.346565089123814	18.542770856687383
35	16.911771710929536	32.204294075243176	33.046151305656736	17.837782908170556
36	16.48211013690104	31.934366257117365	33.027732701596385	18.555790904385216
37	16.77617267758869	32.08933658093547	32.71779205396016	18.41669868751568
38	16.650418070555954	31.207784083150468	33.06806309324577	19.073734753047802
39	16.77903073683943	30.42308803775179	34.54313922876859	18.254741996640192
40	16.361754086230825	31.06805674200299	33.88038704473498	18.689802127031207
41	15.927646642256724	30.430391966948132	34.32148085576645	19.320480535028693
42	15.333487880240968	30.91816741240842	34.00931727315742	19.739027434193186
43	15.429709208349344	31.09282658884277	33.59521624393853	19.88224795886935
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	194.0
1	211.5
2	229.0
3	659.0
4	1089.0
5	1089.0
6	1631.5
7	2174.0
8	2119.0
9	2064.0
10	3017.5
11	3971.0
12	3971.0
13	7329.5
14	10688.0
15	15400.5
16	20113.0
17	16939.0
18	13765.0
19	13765.0
20	14936.5
21	16108.0
22	11500.0
23	6892.0
24	6956.0
25	7020.0
26	7020.0
27	7223.0
28	7426.0
29	7186.5
30	6947.0
31	7207.0
32	7467.0
33	7467.0
34	7904.0
35	8341.0
36	8543.5
37	8746.0
38	10048.0
39	11350.0
40	11350.0
41	12744.0
42	14138.0
43	15900.5
44	17663.0
45	20562.5
46	23462.0
47	23462.0
48	26138.5
49	28815.0
50	25423.0
51	22031.0
52	19988.5
53	17946.0
54	17946.0
55	16661.5
56	15377.0
57	14191.0
58	13005.0
59	11080.0
60	9155.0
61	9155.0
62	7615.0
63	6075.0
64	5079.5
65	4084.0
66	3531.0
67	2978.0
68	2978.0
69	2633.5
70	2289.0
71	1863.5
72	1438.0
73	1172.0
74	906.0
75	906.0
76	705.0
77	504.0
78	388.0
79	272.0
80	183.0
81	94.0
82	94.0
83	70.0
84	46.0
85	36.5
86	27.0
87	16.0
88	5.0
89	5.0
90	4.0
91	3.0
92	2.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	314899.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.089818979958665
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.08230544942901	23.521006199997355
2	12.804010181960408	8.47364758277235
3	5.0015116433049185	4.96497344709346
4	2.635930275872936	3.488898226897118
5	1.6526348823348445	2.7342694548212645
6	1.0880741853389226	2.1602506697778563
7	0.7699245167067679	1.7833664020241373
8	0.5983863660861801	1.584039722709358
9	0.4727251664788263	1.4078151167449664
>10	3.4048178985327193	22.116821208834573
>50	0.32612051915092866	7.414294782043108
>100	0.15059387887397624	8.9452029359532
>500	0.005983862736714287	1.5764492889046127
>1k	0.0039892418244761915	2.4344706728593946
>5k	0.0029919313683571436	7.39449428856723
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8787	2.7904185151429504	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7963	2.528747312630399	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5657	1.7964490201620202	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3667	1.1645003636086493	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1400	0.44458699456016054	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1223	0.38837849596219737	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1087	0.3451900450620675	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	990	0.31438651758182784	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	966	0.30676502624651075	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	871	0.2765966230442142	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	854	0.27119806668169794	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	564	0.1791050463799504	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	532	0.16894305793286102	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	495	0.15719325879091392	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	459	0.14576102178793837	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	455	0.14449077323205217	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	451	0.143220524676166	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	442	0.1403624654254221	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	388	0.12321410992095878	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	370	0.117497991419471	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	352	0.11178187291798322	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	338	0.10733600297238163	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	336	0.10670087869443852	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	334	0.10606575441649545	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	330	0.10479550586060928	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	329	0.10447794372163773	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.175621389715433E-4	0.0
2	0.0	0.0	0.0	3.175621389715433E-4	0.0
3	0.0	0.0	0.0	3.175621389715433E-4	0.0
4	0.0	0.0	0.0	3.175621389715433E-4	0.0
5	0.0	0.0	0.0	3.175621389715433E-4	0.0
6	0.0	0.0	0.0	3.175621389715433E-4	0.0
7	0.0	0.0	0.0	3.175621389715433E-4	0.0
8	0.0	0.0	0.0	3.175621389715433E-4	0.0
9	0.0	0.0	0.0	3.175621389715433E-4	0.0
10	0.0	0.0	0.0	3.175621389715433E-4	0.0
11	0.0	0.0	0.0	6.351242779430866E-4	0.0
12	0.0	0.0	0.0	6.351242779430866E-4	0.0
13	0.0	0.0	0.0	6.351242779430866E-4	0.0
14	0.0	0.0	0.0	0.0012702485558861731	0.0
15	0.0	0.0	0.0	0.0012702485558861731	0.0
16	0.0	0.0	0.0	0.0022229349728008028	0.0
17	0.0	0.0	0.0	0.0060336806404593215	0.0
18	0.0	0.0	0.0	0.007621491335317038	0.0
19	0.0	0.0	0.0	0.00984442630811784	0.0
20	0.0	0.0	0.0	0.0117497991419471	0.0
21	0.0	0.0	0.0	0.016195669087548707	0.0
22	0.0	0.0	0.0	0.02508740897875192	0.0
23	0.0	0.0	0.0	0.03715477025967056	0.0
24	0.0	0.0	0.0	0.053032877208247724	0.0
25	0.0	0.0	0.0	0.05938411998767859	0.0
26	0.0	0.0	0.0	0.07176904340756877	0.0
27	0.0	0.0	0.0	0.10320769516575155	0.0
28	0.0	0.0	0.0	0.16386206370931633	0.0
29	0.0	0.0	0.0	0.2613536403735801	0.0
30	0.0	0.0	0.0	0.4376006275027866	0.0
31	0.0	0.0	0.0	0.8272493720208702	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCTAA	20	0.0018399075	37.0	17
ACGTTTA	25	0.005490359	29.6	26
TGGACGT	25	0.005490359	29.6	26
AGTGGAC	25	0.005490359	29.6	24
TTTCAAT	25	0.005490359	29.6	31
TCACGTT	25	0.005490359	29.6	24
GTATACT	40	5.9266495E-5	27.75	4
CGAGGAG	35	8.85504E-4	26.42857	31
ACTGTGC	85	6.91216E-11	26.117647	8
CTGTGCG	80	9.713403E-10	25.4375	9
GTCGTCA	60	1.3322588E-6	24.666668	24
CATAATC	60	1.3322588E-6	24.666668	37
AGGTCGT	60	1.3322588E-6	24.666668	22
AGTCCTA	45	1.3202333E-4	24.666666	29
CCGTATA	45	1.3202333E-4	24.666666	2
CTGATCA	45	1.3202333E-4	24.666666	4
TACTGTG	115	0.0	24.130436	7
GCATAAT	55	1.8969758E-5	23.545454	36
CGGGATA	55	1.8969758E-5	23.545454	24
GTATTAG	40	0.0019280915	23.125	1
>>END_MODULE