FastQCFastQC Report
Fri 10 Feb 2017
ERR1630307.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630307.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2589102
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT147390.569270735567776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108520.4191414629473848No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT95430.36858339300653276No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49240.19018176958652072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA603.728609E-521.5833322
TTAACGG953.609921E-617.52631635
CGCGCTT752.0685267E-417.26666612
GACGGAC3000.017.2666667
CGAACTA1107.8168887E-716.81818224
GTATTAG5550.016.01
TCGTTTA2200.015.97727330
TCTAGCG1403.4784534E-815.85714328
TAGGACG2450.015.8571434
CGATACA1302.5924237E-715.6538462
CGGACCA3200.015.6093759
CTTATAC13600.015.37132437
ATAGTGC2550.015.2352948
GGCGTAA1101.4531861E-515.13636413
TAACGGC1101.4531861E-515.13636436
ATCCGTA1353.9774386E-715.07407312
AAGACGG4450.014.9662915
TAACGCC2350.014.9574474
GTTCTAG2251.8189894E-1214.81
AATAACG2151.2732926E-1114.6279072
Produced by FastQC (version 0.11.5)