##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630307.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2589102
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.890626557007025	33.0	31.0	34.0	30.0	34.0
2	32.07405115750557	33.0	31.0	34.0	30.0	34.0
3	32.22526304487038	34.0	31.0	34.0	30.0	34.0
4	35.86588245654285	37.0	35.0	37.0	35.0	37.0
5	35.78489568970245	37.0	35.0	37.0	35.0	37.0
6	35.85258904438682	37.0	35.0	37.0	35.0	37.0
7	35.82486862240267	37.0	35.0	37.0	35.0	37.0
8	35.81623358214547	37.0	35.0	37.0	35.0	37.0
9	37.51757945418914	39.0	37.0	39.0	35.0	39.0
10	37.430652017572115	39.0	37.0	39.0	34.0	39.0
11	37.517099751187864	39.0	37.0	39.0	35.0	39.0
12	37.397013713635076	39.0	37.0	39.0	34.0	39.0
13	37.44182191354377	39.0	37.0	39.0	35.0	39.0
14	38.67313145638913	40.0	38.0	41.0	35.0	41.0
15	38.66173599958596	40.0	38.0	41.0	34.0	41.0
16	38.64824251806225	40.0	38.0	41.0	34.0	41.0
17	38.62731402625312	40.0	38.0	41.0	34.0	41.0
18	38.61953642614312	40.0	38.0	41.0	34.0	41.0
19	38.64551222779172	40.0	38.0	41.0	34.0	41.0
20	38.598069137484735	40.0	38.0	41.0	34.0	41.0
21	38.57051595495272	40.0	38.0	41.0	34.0	41.0
22	38.54225750858792	40.0	38.0	41.0	34.0	41.0
23	38.49864779371381	40.0	38.0	41.0	34.0	41.0
24	38.488431510230185	40.0	38.0	41.0	34.0	41.0
25	38.46399910084655	40.0	38.0	41.0	34.0	41.0
26	38.30759352084236	40.0	38.0	41.0	34.0	41.0
27	38.222325346780465	40.0	38.0	41.0	34.0	41.0
28	38.1520585129516	40.0	38.0	41.0	33.0	41.0
29	38.08235789860731	40.0	38.0	41.0	33.0	41.0
30	38.013874694778345	40.0	37.0	41.0	33.0	41.0
31	37.89450280444726	40.0	37.0	41.0	33.0	41.0
32	37.79598138659659	40.0	37.0	41.0	33.0	41.0
33	37.725520276914544	40.0	37.0	41.0	33.0	41.0
34	37.63981836173314	40.0	37.0	41.0	33.0	41.0
35	37.56090219697795	40.0	37.0	41.0	33.0	41.0
36	37.460408666788716	40.0	37.0	41.0	32.0	41.0
37	37.38994948827818	40.0	36.0	41.0	32.0	41.0
38	37.2986336575384	40.0	36.0	41.0	32.0	41.0
39	37.19214963334778	39.0	36.0	41.0	31.0	41.0
40	37.08518590615588	39.0	36.0	41.0	31.0	41.0
41	36.958265066420715	39.0	36.0	41.0	31.0	41.0
42	36.872866345165235	39.0	35.0	41.0	31.0	41.0
43	35.7448972655384	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	7.0
10	12.0
11	14.0
12	4.0
13	6.0
14	8.0
15	11.0
16	13.0
17	25.0
18	60.0
19	135.0
20	283.0
21	567.0
22	1062.0
23	1960.0
24	3387.0
25	5624.0
26	8744.0
27	13235.0
28	19139.0
29	26799.0
30	36813.0
31	49259.0
32	64255.0
33	85924.0
34	114912.0
35	155764.0
36	221429.0
37	341831.0
38	613083.0
39	824735.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.1417920190089	18.57311917413837	12.628934665378189	25.656154141474534
2	17.617730008319487	20.75121026518075	35.29505596921249	26.336003757287273
3	19.070048225214766	22.985421200091768	30.305140546799624	27.63939002789384
4	14.014897829440478	15.44133834819949	34.55935687354148	35.984406948818545
5	14.020073369067731	37.40401112045798	34.33626794154885	14.239647568925443
6	34.722618112380275	35.15724757077937	15.831666732326497	14.288467584513858
7	29.581337467585282	31.180038484385708	20.887666843561973	18.35095720446703
8	28.19873454193771	34.44858487614625	19.0207647284657	18.331915853450347
9	26.951197751189408	14.35717866658015	19.17220719770793	39.51941638452251
10	16.73584122989361	27.58863884080272	32.713890762125246	22.961629167178426
11	36.15060356834145	22.189044695805727	21.096658223584857	20.56369351226796
12	21.387878886192972	25.631628263390166	30.014576482502424	22.965916367914435
13	30.386867724794158	19.581847296862	24.797091810210645	25.234193168133203
14	22.40610837270992	20.074064289471792	25.561835725282357	31.95799161253593
15	24.837916775777856	27.991133605396772	22.322063788912143	24.84888582991323
16	24.395948865668483	26.74448515353972	24.417037258478036	24.44252872231376
17	23.235623779982404	26.889168522522482	26.06162291018276	23.813584787312358
18	23.396953847318493	25.677976379455114	26.953940014723248	23.97112975850314
19	23.926983177951275	25.831156903049784	27.098623383706013	23.14323653529293
20	23.947144608439526	25.708759253208257	26.971127441097337	23.37296869725488
21	24.298270211061595	25.324996852190452	26.720770367486484	23.655962569261465
22	24.371577481304328	25.25620852326405	26.47184236078764	23.90037163464398
23	23.912267651100652	25.676392818822897	26.68141309226133	23.72992643781512
24	23.732436960768638	25.973522866229292	26.37296637984908	23.921073793152992
25	24.016937146547335	25.51880922420206	26.930186605239964	23.53406702401064
26	23.92003096054153	25.858270550947783	26.818101411222887	23.403597077287802
27	23.694779116465863	25.723667897209147	26.614980792568232	23.966572193756754
28	23.636110126213644	25.823702581049336	26.847957322654725	23.69222997008229
29	23.62517969550833	26.069308972763526	27.04014751060406	23.26536382112408
30	23.36288798201075	26.38764328326964	26.68670450217875	23.562764232540857
31	23.859778409657093	26.032500843921945	26.65402908035296	23.453691666068003
32	23.262582934160182	26.27559671268262	26.753600283032497	23.7082200701247
33	23.08425855760028	26.200937622387993	27.076569405145108	23.63823441486662
34	23.855259468340762	25.543026114845997	26.704857514304187	23.896856902509057
35	23.347206869408776	25.928410699926076	27.2815053250123	23.442877105652848
36	23.50992738022681	25.92605467069277	26.686047903867827	23.87797004521259
37	23.377989743161915	25.60594368240417	27.135856370278187	23.880210204155727
38	23.00902011585484	25.79187687468474	27.37331321825096	23.825789791209463
39	23.015083994373338	25.480224417577986	27.763332614937536	23.74135897311114
40	22.930460059124748	25.359642068949	27.885730264779063	23.82416760714719
41	22.427196765519476	25.439747062881263	28.1495669154788	23.983489256120464
42	22.428818949581746	25.551098411727306	28.01314896052763	24.006933678163318
43	22.404640682367862	25.13798220386837	28.325805626815786	24.131571486947983
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	326.0
1	370.0
2	414.0
3	1080.5
4	1747.0
5	1747.0
6	2419.0
7	3091.0
8	2894.5
9	2698.0
10	3620.0
11	4542.0
12	4542.0
13	8627.0
14	12712.0
15	20915.5
16	29119.0
17	26258.0
18	23397.0
19	23397.0
20	26586.5
21	29776.0
22	25757.5
23	21739.0
24	26840.5
25	31942.0
26	31942.0
27	36071.0
28	40200.0
29	48038.5
30	55877.0
31	62427.5
32	68978.0
33	68978.0
34	77623.0
35	86268.0
36	97800.5
37	109333.0
38	122299.5
39	135266.0
40	135266.0
41	148892.5
42	162519.0
43	169732.0
44	176945.0
45	182532.0
46	188119.0
47	188119.0
48	192286.5
49	196454.0
50	196374.5
51	196295.0
52	193681.5
53	191068.0
54	191068.0
55	181194.5
56	171321.0
57	160362.0
58	149403.0
59	142162.5
60	134922.0
61	134922.0
62	123622.5
63	112323.0
64	99528.5
65	86734.0
66	74015.5
67	61297.0
68	61297.0
69	51340.5
70	41384.0
71	34231.0
72	27078.0
73	21737.5
74	16397.0
75	16397.0
76	12781.0
77	9165.0
78	7059.0
79	4953.0
80	3857.0
81	2761.0
82	2761.0
83	1956.5
84	1152.0
85	914.5
86	677.0
87	519.5
88	362.0
89	362.0
90	320.0
91	278.0
92	159.5
93	41.0
94	30.0
95	19.0
96	19.0
97	12.5
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2589102.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.03814985419004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.06184219456125	37.55955708063021
2	13.364073458432552	13.374270207509037
3	4.546394572187592	6.824795186981969
4	2.1915068150322656	4.3863578566825305
5	1.2150469246968256	3.0399350048926266
6	0.7587970276684652	2.2781279627633193
7	0.4991694494939333	1.7484261001487689
8	0.3588265607613996	1.4364013775234035
9	0.28516623273889524	1.2842271618429306
>10	1.5052656682720826	13.982671956238363
>50	0.12874250143002738	4.486838410502411
>100	0.08254559261377396	7.084140224481638
>500	0.0017743837808341793	0.5712470751282925
>1k	6.171769672456371E-4	0.586726874906276
>5k	7.714712090570464E-5	0.36838835234098233
>10k+	1.5429424181140927E-4	0.9878891674272324
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14739	0.569270735567776	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10852	0.4191414629473848	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9543	0.36858339300653276	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4924	0.19018176958652072	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	3.862343005412688E-5
9	0.0	0.0	0.0	3.862343005412688E-5	3.862343005412688E-5
10	0.0	0.0	0.0	7.724686010825376E-5	3.862343005412688E-5
11	0.0	0.0	0.0	7.724686010825376E-5	3.862343005412688E-5
12	0.0	0.0	0.0	3.476108704871419E-4	3.862343005412688E-5
13	0.0	0.0	0.0	3.8623430054126874E-4	3.862343005412688E-5
14	0.0	0.0	0.0	5.407280207577762E-4	3.862343005412688E-5
15	0.0	0.0	0.0	7.338451710284106E-4	3.862343005412688E-5
16	0.0	0.0	0.0	0.0010814560415155525	3.862343005412688E-5
17	0.0	0.0	0.0	0.0016221840622733287	3.862343005412688E-5
18	0.0	0.0	0.0	0.001892548072652217	3.862343005412688E-5
19	0.0	0.0	0.0	0.002162912083031105	3.862343005412688E-5
20	0.0	0.0	0.0	0.0028195103939512617	7.724686010825376E-5
21	0.0	0.0	0.0	0.003437485274817292	1.1587029016238063E-4
22	0.0	0.0	0.0	0.00482792875676586	1.1587029016238063E-4
23	0.0	0.0	0.0	0.007145334560013472	1.1587029016238063E-4
24	0.0	0.0	0.0	0.010544196404776636	1.1587029016238063E-4
25	0.0	0.0	0.0	0.01162565244629219	1.1587029016238063E-4
26	0.0	0.0	0.0	0.013788564529323295	1.1587029016238063E-4
27	0.0	0.0	0.0	0.023560292333017395	1.544937202165075E-4
28	0.0	0.0	0.0	0.053647944345182226	2.703640103788881E-4
29	0.0	0.0	0.0	0.09910772151888957	2.703640103788881E-4
30	0.0	0.0	0.0	0.1648447994710135	2.703640103788881E-4
31	0.0	0.0	0.0	0.36390995796998343	2.703640103788881E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATA	60	3.728609E-5	21.583332	2
TTAACGG	95	3.609921E-6	17.526316	35
CGCGCTT	75	2.0685267E-4	17.266666	12
GACGGAC	300	0.0	17.266666	7
CGAACTA	110	7.8168887E-7	16.818182	24
GTATTAG	555	0.0	16.0	1
TCGTTTA	220	0.0	15.977273	30
TCTAGCG	140	3.4784534E-8	15.857143	28
TAGGACG	245	0.0	15.857143	4
CGATACA	130	2.5924237E-7	15.653846	2
CGGACCA	320	0.0	15.609375	9
CTTATAC	1360	0.0	15.371324	37
ATAGTGC	255	0.0	15.235294	8
GGCGTAA	110	1.4531861E-5	15.136364	13
TAACGGC	110	1.4531861E-5	15.136364	36
ATCCGTA	135	3.9774386E-7	15.074073	12
AAGACGG	445	0.0	14.966291	5
TAACGCC	235	0.0	14.957447	4
GTTCTAG	225	1.8189894E-12	14.8	1
AATAACG	215	1.2732926E-11	14.627907	2
>>END_MODULE