FastQCFastQC Report
Fri 10 Feb 2017
ERR1630306.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630306.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378975
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40831.0773797743914506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31250.8245926512302924No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23120.6100666270862194No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15360.4053037799327132No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6790.1791674912593179No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4500.11874134177716208No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4400.11610264529322514No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4370.11531103634804406No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA3990.1052839897090837No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3870.10211755392835939No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGT250.005491753629.5999984
CCTAAGC400.001928812423.1253
AGTACCT450.003821148520.5555555
GGTATCA15800.017.4462031
ACCTTCA752.0626633E-417.26666610
TCCTAAG650.001577293617.0769232
ATACAGT650.001577293617.0769236
TCTTATA3600.016.95833437
TAACACT1107.7736877E-716.8181824
CGCTGTC1005.856904E-616.6535
AACACTC700.00258819715.8571425
AGTCACC855.3523754E-415.2352946
CGGCCTT855.3523754E-415.23529424
TGTATAA855.3523754E-415.23529410
TAAGTCA855.3523754E-415.2352944
TAATCTA855.3523754E-415.23529420
ACTATTC1101.4466894E-515.1363648
CAGATGT750.004097659614.7999994
GCCCTTA750.004097659614.79999932
AGCTGGC750.004097659614.79999915
Produced by FastQC (version 0.11.5)