##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630306.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	378975
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.910400422191437	33.0	31.0	34.0	30.0	34.0
2	32.112920377333595	33.0	31.0	34.0	30.0	34.0
3	32.20077577676628	34.0	31.0	34.0	30.0	34.0
4	35.84300547529521	37.0	35.0	37.0	35.0	37.0
5	35.78027310508609	37.0	35.0	37.0	35.0	37.0
6	35.86239725575566	37.0	35.0	37.0	35.0	37.0
7	35.84713503529257	37.0	35.0	37.0	35.0	37.0
8	35.87920311366185	37.0	35.0	37.0	35.0	37.0
9	37.601345735206806	39.0	37.0	39.0	35.0	39.0
10	37.51904215317633	39.0	37.0	39.0	35.0	39.0
11	37.58770103568837	39.0	37.0	39.0	35.0	39.0
12	37.54632363612375	39.0	37.0	39.0	35.0	39.0
13	37.5493053631506	39.0	37.0	39.0	35.0	39.0
14	38.753783231083844	40.0	38.0	41.0	35.0	41.0
15	38.78086945049146	40.0	38.0	41.0	35.0	41.0
16	38.700996107922684	40.0	38.0	41.0	34.0	41.0
17	38.73502473777954	40.0	38.0	41.0	35.0	41.0
18	38.72582360314005	40.0	38.0	41.0	35.0	41.0
19	38.78802295665941	40.0	38.0	41.0	35.0	41.0
20	38.77833102447391	40.0	38.0	41.0	35.0	41.0
21	38.73295599973613	40.0	38.0	41.0	35.0	41.0
22	38.69787189128571	40.0	38.0	41.0	34.0	41.0
23	38.63065109835741	40.0	38.0	41.0	34.0	41.0
24	38.589170789629925	40.0	38.0	41.0	34.0	41.0
25	38.58054225212745	40.0	38.0	41.0	34.0	41.0
26	38.408095520812715	40.0	38.0	41.0	34.0	41.0
27	38.27510785671878	40.0	38.0	41.0	34.0	41.0
28	38.22254502275876	40.0	38.0	41.0	34.0	41.0
29	38.1439672801636	40.0	38.0	41.0	34.0	41.0
30	38.047884425094004	40.0	37.0	41.0	33.0	41.0
31	37.935088066495155	40.0	37.0	41.0	33.0	41.0
32	37.80636189722277	40.0	37.0	41.0	33.0	41.0
33	37.692921696681836	40.0	37.0	41.0	33.0	41.0
34	37.60403720562042	40.0	36.0	41.0	33.0	41.0
35	37.46820766541329	40.0	36.0	41.0	33.0	41.0
36	37.34892275216043	40.0	36.0	41.0	32.0	41.0
37	37.261244145392176	40.0	36.0	41.0	32.0	41.0
38	37.12485256283396	40.0	36.0	41.0	31.0	41.0
39	36.991545616465466	40.0	36.0	41.0	31.0	41.0
40	36.84953097169998	39.0	35.0	41.0	31.0	41.0
41	36.690407018932646	39.0	35.0	41.0	30.0	41.0
42	36.59178309914902	39.0	35.0	41.0	30.0	41.0
43	35.508466257668715	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	2.0
12	0.0
13	0.0
14	0.0
15	1.0
16	1.0
17	6.0
18	8.0
19	27.0
20	57.0
21	85.0
22	210.0
23	322.0
24	558.0
25	933.0
26	1449.0
27	2118.0
28	2820.0
29	4050.0
30	5586.0
31	7194.0
32	9485.0
33	12549.0
34	16394.0
35	21863.0
36	31108.0
37	49237.0
38	83739.0
39	129169.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.177650240781055	22.000395804472593	14.510719704465993	22.31123425028036
2	17.38109373969259	23.391516590804144	36.49475559073817	22.73263407876509
3	19.55142159773072	25.352859687314467	32.63460650438683	22.46111221056798
4	14.463487037403521	17.715152714559007	35.8955076192361	31.925852628801373
5	12.596081535721353	38.643710007256416	35.13134111748796	13.62886733953427
6	30.022824724586055	36.818523649317235	17.27660135892869	15.882050267168019
7	25.06022824724586	33.96160696615872	23.17699056666007	17.80117421993535
8	25.386898871957253	35.40312685533347	21.590078501220397	17.619895771488885
9	25.13147305231216	15.75407348769708	23.19203113661851	35.92242232337225
10	17.065505640213736	29.218286166633682	34.10119401015898	19.6150141829936
11	30.652417705653406	24.91879411570684	23.068276271521867	21.360511907117885
12	18.873276601358928	29.4853222508081	30.851375420542254	20.79002572729072
13	28.061217758427336	22.284847285440993	27.756448314532623	21.897486641599052
14	21.192163071442707	24.6736592123491	28.336697671350354	25.79748004485784
15	24.151197308529586	29.77505112474438	25.46685137542054	20.606900191305495
16	21.455241110891222	29.056006332871558	27.929546803878885	21.559205752358334
17	20.20449897750511	30.004617718846887	28.843591265914636	20.94729203773336
18	19.17250478263738	27.580183389405633	30.999406293291116	22.247905534665875
19	20.536974734481166	28.16861270532357	32.17019592321393	19.12421663698133
20	21.1631374101194	27.09782967214196	32.07071706576951	19.668315851969126
21	21.127515007586254	27.835345339402334	30.66297249158916	20.374167161422257
22	21.214064252259384	27.87202322052906	30.591463816874466	20.322448710337092
23	21.06576950986213	27.869648393693513	30.679332409789563	20.385249686654795
24	20.686588825120392	28.578138399630582	30.763242957978758	19.97202981727027
25	20.860478923411836	27.803153242298308	30.88040108186556	20.455966752424303
26	20.53671086483277	28.217428590276405	30.594894122303586	20.650966422587242
27	20.51006003034501	28.43855135563032	30.177188468896365	20.874200145128306
28	20.234843987070388	28.465729929414866	30.89333069463685	20.406095388877894
29	20.307672010027048	28.62563493634145	30.9373969259186	20.12929612771291
30	20.082591199947224	29.454977241242826	30.48169404314269	19.98073751566726
31	20.278910218352134	28.85309057325681	30.851111550893854	20.016887657497197
32	19.714493040438025	29.00587109967676	30.631044264133518	20.648591595751697
33	19.843789168150934	28.891879411570688	30.926578270334453	20.33775314994393
34	20.192360973679	28.34487763045056	30.627877828352794	20.834883567517647
35	20.088660201860282	28.648855465400093	30.793324097895642	20.469160234843987
36	20.080744112408468	28.810871429513817	30.405435714756912	20.7029487433208
37	20.39791542977769	27.785737845504322	30.601226993865033	21.215119730852958
38	20.05778745299822	28.171251401807506	31.22052905864503	20.550432086549243
39	19.575697605382942	28.169404314268753	31.61791674912593	20.636981331222376
40	19.535061679530312	27.446665347318426	32.08813246256349	20.93014051058777
41	18.98858763770697	27.993403258790156	32.1506695692328	20.867339534270073
42	19.18701761329903	27.34797809881918	31.90764562306221	21.557358664819578
43	18.527079622666403	27.689161554192225	31.948281548914835	21.83547727422653
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	531.0
1	410.5
2	290.0
3	523.5
4	757.0
5	757.0
6	937.0
7	1117.0
8	1068.0
9	1019.0
10	1460.0
11	1901.0
12	1901.0
13	3282.0
14	4663.0
15	6460.5
16	8258.0
17	7642.5
18	7027.0
19	7027.0
20	8458.0
21	9889.0
22	8887.5
23	7886.0
24	8682.0
25	9478.0
26	9478.0
27	10353.5
28	11229.0
29	13375.0
30	15521.0
31	16584.5
32	17648.0
33	17648.0
34	18595.5
35	19543.0
36	20819.5
37	22096.0
38	22894.5
39	23693.0
40	23693.0
41	24846.0
42	25999.0
43	25193.5
44	24388.0
45	24318.5
46	24249.0
47	24249.0
48	23945.0
49	23641.0
50	23352.5
51	23064.0
52	23094.0
53	23124.0
54	23124.0
55	20528.0
56	17932.0
57	16426.0
58	14920.0
59	13149.5
60	11379.0
61	11379.0
62	10315.5
63	9252.0
64	7963.5
65	6675.0
66	5517.0
67	4359.0
68	4359.0
69	3575.0
70	2791.0
71	2385.5
72	1980.0
73	1619.0
74	1258.0
75	1258.0
76	910.5
77	563.0
78	503.0
79	443.0
80	308.5
81	174.0
82	174.0
83	135.5
84	97.0
85	87.0
86	77.0
87	57.0
88	37.0
89	37.0
90	31.5
91	26.0
92	13.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	378975.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.40765584036832
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.71345939799977	44.16735924169545
2	11.773234329927421	13.046546317612556
3	3.5644040693855343	5.92485821857566
4	1.5634709723987223	3.465130462202976
5	0.7822729044818754	2.1671953932388535
6	0.4650236057607132	1.5459520743382031
7	0.2995544328290167	1.1618326243752823
8	0.24022456702000153	1.064822410707659
9	0.18531253684938198	0.9240959938190516
>10	1.2207869566843454	13.417249577590928
>50	0.13643990279326298	5.04075788191455
>100	0.05343101088405768	4.972296618598354
>500	4.7706259717908645E-4	0.1794795281499273
>1k	0.0019082503887163458	2.922423657180554
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4083	1.0773797743914506	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3125	0.8245926512302924	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2312	0.6100666270862194	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1536	0.4053037799327132	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	679	0.1791674912593179	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	450	0.11874134177716208	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	440	0.11610264529322514	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	437	0.11531103634804406	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	399	0.1052839897090837	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	387	0.10211755392835939	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.638696483936935E-4	0.0
10	0.0	0.0	0.0	5.27739296787387E-4	0.0
11	0.0	0.0	0.0	5.27739296787387E-4	0.0
12	0.0	0.0	0.0	5.27739296787387E-4	0.0
13	0.0	0.0	0.0	5.27739296787387E-4	0.0
14	0.0	0.0	0.0	5.27739296787387E-4	0.0
15	0.0	0.0	0.0	7.916089451810805E-4	0.0
16	0.0	0.0	0.0	0.001055478593574774	0.0
17	0.0	0.0	0.0	0.001583217890362161	0.0
18	0.0	0.0	0.0	0.0018470875387558547	0.0
19	0.0	0.0	0.0	0.002110957187149548	0.0
20	0.0	0.0	0.0	0.002374826835543242	0.0
21	0.0	0.0	0.0	0.003166435780724322	0.0
22	0.0	0.0	0.0	0.004749653671086484	0.0
23	0.0	0.0	0.0	0.006596741209842338	0.0
24	0.0	0.0	0.0	0.01055478593574774	0.0
25	0.0	0.0	0.0	0.01134639488092882	0.0
26	0.0	0.0	0.0	0.01741539679398377	0.0
27	0.0	0.0	0.0	0.04195527409459727	0.0
28	0.0	0.0	0.0	0.08048024276007652	0.0
29	0.0	0.0	0.0	0.1250742133386107	0.0
30	0.0	0.0	0.0	0.20660993469226202	0.0
31	0.0	0.0	0.0	0.46572992941486907	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGAGT	25	0.0054917536	29.599998	4
CCTAAGC	40	0.0019288124	23.125	3
AGTACCT	45	0.0038211485	20.555555	5
GGTATCA	1580	0.0	17.446203	1
ACCTTCA	75	2.0626633E-4	17.266666	10
TCCTAAG	65	0.0015772936	17.076923	2
ATACAGT	65	0.0015772936	17.076923	6
TCTTATA	360	0.0	16.958334	37
TAACACT	110	7.7736877E-7	16.818182	4
CGCTGTC	100	5.856904E-6	16.65	35
AACACTC	70	0.002588197	15.857142	5
AGTCACC	85	5.3523754E-4	15.235294	6
CGGCCTT	85	5.3523754E-4	15.235294	24
TGTATAA	85	5.3523754E-4	15.235294	10
TAAGTCA	85	5.3523754E-4	15.235294	4
TAATCTA	85	5.3523754E-4	15.235294	20
ACTATTC	110	1.4466894E-5	15.136364	8
CAGATGT	75	0.0040976596	14.799999	4
GCCCTTA	75	0.0040976596	14.799999	32
AGCTGGC	75	0.0040976596	14.799999	15
>>END_MODULE