FastQCFastQC Report
Fri 10 Feb 2017
ERR1630305.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630305.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247593
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28510.22852003818553007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22480.18018696802563017No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA22400.1795457332639731No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19340.15501850363059108No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA13550.1086091377556623No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC12790.10251740751992036No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAT250.00549652229.59999814
TCGCACG701.9231993E-723.78571322
CTCGCTA450.003825988420.55555513
AATGCGT656.902733E-519.92307735
CGTATTA851.245482E-619.58823615
TAGCGGG951.6763988E-719.47368428
CCGATCG801.6168515E-518.518
TCTATAC1156.406117E-817.6956523
TATACAG953.6068868E-617.5263165
GTATTGG1401.869921E-917.1785721
GGTATCA12300.016.9959351
TTAGACT1107.8089033E-716.8181824
CGTTATT2900.016.5862062
ACGGACC2800.016.5178578
TAATACG904.4464457E-516.4444454
GATATAC1708.54925E-1116.323533
TAGAACA2950.016.3050864
TAATGGT803.382856E-416.187516
AATACGC803.382856E-416.18755
AAATGCG803.382856E-416.187534
Produced by FastQC (version 0.11.5)