##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630305.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1247593
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84055136570981	33.0	31.0	34.0	30.0	34.0
2	32.05944727166632	33.0	31.0	34.0	30.0	34.0
3	32.18479183515778	34.0	31.0	34.0	30.0	34.0
4	35.82977541554016	37.0	35.0	37.0	35.0	37.0
5	35.75877149038188	37.0	35.0	37.0	35.0	37.0
6	35.81867564181588	37.0	35.0	37.0	35.0	37.0
7	35.80398014416561	37.0	35.0	37.0	35.0	37.0
8	35.80488268209264	37.0	35.0	37.0	35.0	37.0
9	37.547191271512425	39.0	37.0	39.0	35.0	39.0
10	37.438753664055504	39.0	37.0	39.0	34.0	39.0
11	37.501064048932626	39.0	37.0	39.0	35.0	39.0
12	37.42098264417963	39.0	37.0	39.0	34.0	39.0
13	37.42737174703609	39.0	37.0	39.0	34.0	39.0
14	38.64618749864739	40.0	38.0	41.0	34.0	41.0
15	38.66244039522505	40.0	38.0	41.0	34.0	41.0
16	38.6119607917005	40.0	38.0	41.0	34.0	41.0
17	38.59249691205385	40.0	38.0	41.0	34.0	41.0
18	38.54662618337871	40.0	38.0	41.0	34.0	41.0
19	38.57616305958754	40.0	38.0	41.0	34.0	41.0
20	38.5537807602319	40.0	38.0	41.0	34.0	41.0
21	38.4923264237616	40.0	38.0	41.0	34.0	41.0
22	38.46075923798867	40.0	38.0	41.0	34.0	41.0
23	38.389049153049115	40.0	38.0	41.0	34.0	41.0
24	38.33791388698077	40.0	38.0	41.0	34.0	41.0
25	38.316108698910625	40.0	38.0	41.0	34.0	41.0
26	38.15527499753525	40.0	38.0	41.0	33.0	41.0
27	38.04828096983552	40.0	38.0	41.0	33.0	41.0
28	37.95433526799204	40.0	37.0	41.0	33.0	41.0
29	37.896962390779684	40.0	37.0	41.0	33.0	41.0
30	37.82073160077044	40.0	37.0	41.0	33.0	41.0
31	37.76920117378023	40.0	37.0	41.0	33.0	41.0
32	37.6845766207409	40.0	37.0	41.0	32.0	41.0
33	37.582815870239735	40.0	37.0	41.0	32.0	41.0
34	37.52823156269713	40.0	37.0	41.0	32.0	41.0
35	37.38555121742427	40.0	37.0	41.0	31.0	41.0
36	37.301862065593504	40.0	36.0	41.0	31.0	41.0
37	37.251341583352904	39.0	36.0	41.0	31.0	41.0
38	37.13134331468676	39.0	36.0	41.0	31.0	41.0
39	37.05025597290142	39.0	36.0	41.0	31.0	41.0
40	36.940681776829464	39.0	36.0	41.0	31.0	41.0
41	36.81984108599519	39.0	35.0	41.0	30.0	41.0
42	36.77603433170914	39.0	35.0	41.0	30.0	41.0
43	35.515963940163175	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	3.0
11	2.0
12	1.0
13	3.0
14	1.0
15	3.0
16	5.0
17	29.0
18	59.0
19	100.0
20	232.0
21	396.0
22	732.0
23	1302.0
24	2051.0
25	3198.0
26	4923.0
27	7210.0
28	10171.0
29	14165.0
30	19115.0
31	25169.0
32	32347.0
33	42000.0
34	56206.0
35	76179.0
36	106790.0
37	162075.0
38	296782.0
39	386338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.81300071417522	19.259405912024192	12.114126962879721	25.81346641092087
2	18.6592101751132	21.356003119607113	33.69776842287509	26.2870182824046
3	19.53529716822714	23.157391873792175	30.495121405778967	26.81218955220172
4	14.592980242755448	15.718427403808773	35.01718909932967	34.67140325410611
5	14.237816339142654	37.25718243048815	34.30589943996159	14.199101790407608
6	33.90264292922451	35.86826793673899	15.942058026936671	14.287031107099832
7	29.21257172811967	31.01075430849644	21.03185894758948	18.744815015794416
8	28.14876325853063	32.92748516543456	19.74858788082331	19.1751636952115
9	26.905328901332403	14.120229914723792	19.125548155528286	39.84889302841552
10	17.41184825499983	26.381840872784636	32.46403274144693	23.742278130768607
11	36.37885111570841	21.96461506276486	21.11385684273637	20.542676978790357
12	21.729762831307966	25.733873146130186	28.477075456499033	24.05928856606281
13	30.37208448588602	19.139495011594327	24.980502455528367	25.507918046991286
14	23.310486673137795	20.485526930657674	24.132068711510886	32.071917684693645
15	25.769301366711737	27.52524260716436	21.631814221464854	25.073641804659054
16	25.752308645527826	25.892578749640304	23.493959969316915	24.861152635514948
17	24.47440791989054	26.24750219021748	24.575642857887146	24.70244703200483
18	24.038769053689784	24.543020039387844	26.140255676330344	25.27795523059203
19	24.798471937562972	25.008235858970036	26.051364507495634	24.14192769597136
20	25.041339603540578	24.342153250298775	25.609633911059138	25.006873235101512
21	25.500143075506195	25.22288919543473	24.62285376721415	24.654113961844928
22	25.860517011557455	24.506469657973394	24.867404674441104	24.76560865602805
23	24.880309524019452	24.767372051622605	25.433374505948652	24.91894391840929
24	24.97481149701866	25.159727571411512	25.181609707653056	24.683851223916776
25	25.259279268158767	24.255666711820282	25.440428088326883	25.04462593169407
26	24.65010624458457	25.54438827406053	25.254229544410716	24.55127593694418
27	25.549277689118167	24.535725993973998	24.82307932154156	25.091916995366276
28	24.325160529114864	25.086386345546984	26.139373978533065	24.44907914680509
29	24.531798431058846	25.179445540332463	25.71680027060107	24.57195575800762
30	23.897937869160856	26.39867328527813	25.541903489359107	24.161485356201904
31	24.788693107447703	25.65299741181619	25.23643527977473	24.32187420096137
32	24.438338464547332	25.46190945284239	24.870370385213768	25.22938169739651
33	24.110346883959753	25.075725817634435	25.576530166488592	25.237397131917223
34	25.08574511078533	24.72448947693679	25.340635928544003	24.84912948373388
35	24.98346816630103	24.98146430767085	26.063788431002738	23.97127909502538
36	23.96093918449366	25.691471497515618	25.55023954126065	24.797349776730073
37	25.451810005346292	24.572516838424068	25.333662500510982	24.642010655718654
38	24.112831668661176	25.17391489051317	26.171435716615914	24.54181772420974
39	24.659804920354635	24.903955055855555	25.9104531686215	24.525786855168313
40	24.30680518406243	24.410685215450872	26.817800356366217	24.464709244120478
41	23.36731610388965	25.097287336495157	27.05241212478749	24.482984434827703
42	24.229776858318377	24.23418534730477	26.904447203535124	24.631590590841725
43	23.5398884091206	23.752457732609912	27.119661620416274	25.587992237853207
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	95.0
1	82.5
2	70.0
3	167.0
4	264.0
5	264.0
6	372.5
7	481.0
8	459.0
9	437.0
10	693.5
11	950.0
12	950.0
13	1620.5
14	2291.0
15	4411.0
16	6531.0
17	6381.5
18	6232.0
19	6232.0
20	7120.5
21	8009.0
22	9007.5
23	10006.0
24	11903.0
25	13800.0
26	13800.0
27	15877.5
28	17955.0
29	24293.5
30	30632.0
31	32227.5
32	33823.0
33	33823.0
34	37077.5
35	40332.0
36	44774.5
37	49217.0
38	54970.5
39	60724.0
40	60724.0
41	68789.5
42	76855.0
43	76043.0
44	75231.0
45	79696.5
46	84162.0
47	84162.0
48	88347.5
49	92533.0
50	91693.5
51	90854.0
52	96451.0
53	102048.0
54	102048.0
55	94690.0
56	87332.0
57	82868.0
58	78404.0
59	72411.0
60	66418.0
61	66418.0
62	63323.5
63	60229.0
64	54815.5
65	49402.0
66	43013.0
67	36624.0
68	36624.0
69	30755.5
70	24887.0
71	21283.0
72	17679.0
73	13932.5
74	10186.0
75	10186.0
76	7784.5
77	5383.0
78	4482.0
79	3581.0
80	2705.0
81	1829.0
82	1829.0
83	1350.0
84	871.0
85	728.5
86	586.0
87	474.0
88	362.0
89	362.0
90	287.5
91	213.0
92	130.0
93	47.0
94	33.0
95	19.0
96	19.0
97	11.5
98	4.0
99	4.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1247593.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.31532542386451
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.79541589544944	32.4962301467696
2	12.598656207687894	10.662324746634079
3	4.110317743855055	5.217882987801335
4	1.805119069645238	3.0553680344344714
5	0.9865442830859664	2.087297119191789
6	0.6334029517503802	1.6081591216652213
7	0.4152564216513351	1.2300197431568016
8	0.30666798448811805	1.0381404448556277
9	0.24373570872011294	0.9282380248717047
>10	1.666530633385362	13.997072030294444
>50	0.23461883391448032	6.904717020630176
>100	0.18774017515800498	15.240944320790554
>500	0.013138003492885138	3.761933748318241
>1k	0.002856087715844595	1.771672510585976
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2851	0.22852003818553007	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2248	0.18018696802563017	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2240	0.1795457332639731	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1934	0.15501850363059108	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	1355	0.1086091377556623	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	1279	0.10251740751992036	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	8.015434520713086E-5	0.0
10	0.0	0.0	0.0	8.015434520713086E-5	0.0
11	0.0	2.4046303562139256E-4	0.0	8.015434520713086E-5	0.0
12	0.0	2.4046303562139256E-4	0.0	8.015434520713086E-5	0.0
13	0.0	2.4046303562139256E-4	0.0	8.015434520713086E-5	0.0
14	0.0	2.4046303562139256E-4	0.0	8.015434520713086E-5	0.0
15	0.0	2.4046303562139256E-4	0.0	1.6030869041426172E-4	0.0
16	0.0	2.4046303562139256E-4	0.0	2.4046303562139256E-4	0.0
17	0.0	2.4046303562139256E-4	0.0	3.2061738082852343E-4	0.0
18	0.0	2.4046303562139256E-4	0.0	4.0077172603565425E-4	0.0
19	0.0	2.4046303562139256E-4	0.0	5.610804164499159E-4	0.0
20	0.0	2.4046303562139256E-4	0.0	8.015434520713085E-4	0.0
21	0.0	2.4046303562139256E-4	0.0	8.816977972784393E-4	0.0
22	0.0	2.4046303562139256E-4	0.0	0.0013626238685212244	0.0
23	0.0	2.4046303562139256E-4	0.0	0.0019237042849711405	0.0
24	0.0	3.2061738082852343E-4	0.0	0.003206173808285234	0.0
25	0.0	3.2061738082852343E-4	0.0	0.003687099879528019	0.0
26	0.0	3.2061738082852343E-4	0.0	0.005129878093256375	0.0
27	0.0	3.2061738082852343E-4	0.0	0.009538367079648571	0.0
28	0.0	3.2061738082852343E-4	0.0	0.02540892743066048	0.0
29	0.0	3.2061738082852343E-4	0.0	0.04769183539824286	0.0
30	0.0	3.2061738082852343E-4	0.0	0.08095588865920215	0.0
31	0.0	3.2061738082852343E-4	0.0	0.1737746204090597	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACTAT	25	0.005496522	29.599998	14
TCGCACG	70	1.9231993E-7	23.785713	22
CTCGCTA	45	0.0038259884	20.555555	13
AATGCGT	65	6.902733E-5	19.923077	35
CGTATTA	85	1.245482E-6	19.588236	15
TAGCGGG	95	1.6763988E-7	19.473684	28
CCGATCG	80	1.6168515E-5	18.5	18
TCTATAC	115	6.406117E-8	17.695652	3
TATACAG	95	3.6068868E-6	17.526316	5
GTATTGG	140	1.869921E-9	17.178572	1
GGTATCA	1230	0.0	16.995935	1
TTAGACT	110	7.8089033E-7	16.818182	4
CGTTATT	290	0.0	16.586206	2
ACGGACC	280	0.0	16.517857	8
TAATACG	90	4.4464457E-5	16.444445	4
GATATAC	170	8.54925E-11	16.32353	3
TAGAACA	295	0.0	16.305086	4
TAATGGT	80	3.382856E-4	16.1875	16
AATACGC	80	3.382856E-4	16.1875	5
AAATGCG	80	3.382856E-4	16.1875	34
>>END_MODULE