FastQCFastQC Report
Fri 10 Feb 2017
ERR1630304.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630304.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1057580
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT114441.0820930804289037No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT80820.7641975075171619No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT73430.6943209969931353No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30220.2857467047410125No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC11340.10722593089884454No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGAT11190.10580759847954764No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG10660.10079615726469866No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGAC250.00549614829.59999825
ACGTTTA701.9226172E-723.78571526
TTGGTCG400.00193108623.12510
AATACTG2400.022.3541665
CTCTAAT2550.021.7647061
TTTGAAC603.7259248E-521.5833323
AAAGGAG603.7259248E-521.5833325
TGTTTAC1750.021.14285714
TTACTTA1151.3460522E-1020.91304429
GGTCACA806.959035E-720.812535
ACAAGGA806.959035E-720.81253
GGTATTG1700.020.67647233
ATAGCAT450.003825608420.5555576
CGTAGCG450.003825608420.55555715
CAGTCAT450.003825608420.55555720
CGACTAT450.003825608420.55555736
TTTAGCG555.14272E-420.18181826
ACTCAAG555.14272E-420.18181811
CGTTTGG1750.020.08571428
TTAGAGT1202.382876E-1020.0416664
Produced by FastQC (version 0.11.5)