FastQCFastQC Report
Fri 10 Feb 2017
ERR1630303.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630303.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214400
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT55300.455368906455863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46320.3814229249011858No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35910.29570158102766797No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24400.20092226613965744No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA15400.12681159420289853No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCGA451.3232723E-424.66666820
GTACTAG701.9231084E-723.7857131
GTTCGGT400.001931250323.12511
GGTATCA20500.020.4853671
GACGGAC1450.020.4137927
GGCGTCG500.007035106418.58
TGCGACG609.2372246E-418.522
TCGAACG500.007035106418.53
GCGAAAG1653.6379788E-1217.93939418
ACGGACC1552.0008883E-1117.9032258
AAGACGG1705.456968E-1217.4117645
AGACGGA1705.456968E-1217.4117646
CGGACCA1705.456968E-1217.4117649
TACTAGG1655.4569682E-1116.8181822
CGCAAGA1655.4569682E-1116.8181822
CGAATTA1801.0913936E-1116.44444515
TATACTG1801.0913936E-1116.4444455
GTAAACG904.446289E-516.44444527
GCGGTTC1151.2422606E-616.08695611
CGAAAGC1851.8189894E-1116.019
Produced by FastQC (version 0.11.5)