FastQCFastQC Report
Fri 10 Feb 2017
ERR1630302.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630302.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651362
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33030.20001671347651212No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29180.17670262486359745No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23230.1406717606436384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12350.020.0728761
AAGACGG2000.017.5749995
CGGGTCG650.001580329317.07692334
CGCAAGA2300.016.8913042
ACGGACC2100.016.7380948
TTAGTAC1005.880922E-616.653
GACGGAC2250.016.4444457
GTGCTAG2150.016.3488371
TACACCG1251.658882E-716.285
CAAGACG2400.016.18754
CGGACCA2300.016.0869569
GTATAGA1902.7284841E-1115.5789481
CGCGGTT1201.936649E-615.41666710
GAGTCGT1252.9604544E-614.8000016
TTATAGT2400.014.6458344
CTTATAC5800.014.35344837
ATCTCGC2850.014.28070211
CAATACG1051.6563223E-414.0952384
GCGAAAG2500.014.06000118
ATATACT2252.7284841E-1113.97777754
Produced by FastQC (version 0.11.5)