FastQCFastQC Report
Fri 10 Feb 2017
ERR1630301.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630301.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031274
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26520.130558457401611No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23210.11426326532018821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11650.019.0557961
CTGCGTA650.001580498617.0769239
TCCTATA1251.6594277E-716.2799992
TCTAGCG2300.016.08695628
GACGGAC4100.015.7926847
AAGACGG5050.015.7524765
TATACTT2350.015.7446815
ATACCGC3050.015.16393527
GTCTTAC2109.094947E-1214.97619151
CTAGCGG2500.014.79999929
ACGGACC4400.014.7159098
CTATACT2900.014.6724154
CGGGTAA2400.014.64583324
TATACTG2950.014.423735
CGGACCA4500.014.3888899
CGTTATT2201.8189894E-1114.2954542
CGCAATA2600.014.23076936
TACACCG1304.4477183E-614.2307695
TACCGCA3300.014.01515128
ATACCGT3150.013.5079376
Produced by FastQC (version 0.11.5)