##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630301.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2031274
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.943763372149697	33.0	31.0	34.0	30.0	34.0
2	32.148781503627774	33.0	31.0	34.0	30.0	34.0
3	32.28542038149457	34.0	31.0	34.0	30.0	34.0
4	35.903340465146506	37.0	35.0	37.0	35.0	37.0
5	35.82867353198042	37.0	35.0	37.0	35.0	37.0
6	35.87740698694514	37.0	35.0	37.0	35.0	37.0
7	35.8513440333505	37.0	35.0	37.0	35.0	37.0
8	35.84988189678005	37.0	35.0	37.0	35.0	37.0
9	37.61191400076996	39.0	37.0	39.0	35.0	39.0
10	37.49942794522059	39.0	37.0	39.0	35.0	39.0
11	37.56756892472409	39.0	37.0	39.0	35.0	39.0
12	37.49019580814799	39.0	37.0	39.0	35.0	39.0
13	37.49826906660549	39.0	37.0	39.0	35.0	39.0
14	38.7675803461276	40.0	38.0	41.0	35.0	41.0
15	38.77651759437673	40.0	38.0	41.0	35.0	41.0
16	38.738517304903226	40.0	38.0	41.0	35.0	41.0
17	38.69917352361129	40.0	38.0	41.0	35.0	41.0
18	38.652331000150646	40.0	38.0	41.0	34.0	41.0
19	38.66968907198142	40.0	38.0	41.0	34.0	41.0
20	38.64686103401117	40.0	38.0	41.0	34.0	41.0
21	38.59031671748863	40.0	38.0	41.0	34.0	41.0
22	38.557123755830084	40.0	38.0	41.0	34.0	41.0
23	38.50117660148262	40.0	38.0	41.0	34.0	41.0
24	38.4630374828802	40.0	38.0	41.0	34.0	41.0
25	38.44388053999608	40.0	38.0	41.0	34.0	41.0
26	38.28970586932142	40.0	38.0	41.0	34.0	41.0
27	38.17468692062223	40.0	38.0	41.0	33.0	41.0
28	38.11629302595317	40.0	38.0	41.0	33.0	41.0
29	38.055415468321854	40.0	38.0	41.0	33.0	41.0
30	37.994629971141265	40.0	38.0	41.0	33.0	41.0
31	37.94014199955299	40.0	37.0	41.0	33.0	41.0
32	37.8643910176569	40.0	37.0	41.0	33.0	41.0
33	37.77174866610807	40.0	37.0	41.0	33.0	41.0
34	37.71241939787542	40.0	37.0	41.0	33.0	41.0
35	37.59875181782468	40.0	37.0	41.0	32.0	41.0
36	37.51626073095013	40.0	37.0	41.0	32.0	41.0
37	37.4490172177658	40.0	37.0	41.0	32.0	41.0
38	37.34085800340082	40.0	37.0	41.0	31.0	41.0
39	37.26509963697659	40.0	36.0	41.0	31.0	41.0
40	37.16387055611404	39.0	36.0	41.0	31.0	41.0
41	37.052952482038364	39.0	36.0	41.0	31.0	41.0
42	37.00035347274666	39.0	36.0	41.0	31.0	41.0
43	35.74481335358991	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	7.0
10	3.0
11	5.0
12	4.0
13	1.0
14	3.0
15	2.0
16	11.0
17	30.0
18	53.0
19	145.0
20	301.0
21	586.0
22	968.0
23	1804.0
24	3013.0
25	4644.0
26	7133.0
27	10636.0
28	15358.0
29	21303.0
30	28676.0
31	37866.0
32	49851.0
33	65163.0
34	87115.0
35	117327.0
36	165312.0
37	252616.0
38	477923.0
39	683415.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.34924781196431	18.255341229199015	11.569192536309725	26.82621842252695
2	19.004575453631563	20.0799104404428	33.87632589202638	27.03918821389926
3	20.000600608288195	22.193214701709373	28.79129058905889	29.014894100943543
4	14.724256796473542	14.957952496807422	33.9351559661572	36.382634740561834
5	15.458475813701156	35.532970933512665	33.65365775370531	15.354895499080873
6	36.12225627857197	34.24195849501348	15.234921532004053	14.400863694410504
7	30.71791397910868	29.413412469218827	20.75653013822852	19.112143413443977
8	28.236515605477152	33.134377735352295	19.20129928311001	19.427807376060542
9	27.298532841950422	13.642571115467437	18.586069629208072	40.47282641337407
10	17.118566968316436	25.77594160118231	32.82787058762137	24.277620842879887
11	37.40322575880949	20.91037447434467	20.818166333050094	20.868233433795737
12	22.104797284856694	24.339700109389476	29.176566036881287	24.37893656887254
13	31.050808507370252	19.003492389505304	24.11171511081223	25.833983992312216
14	23.306604623502295	19.529270792615865	24.204957086045507	32.95916749783633
15	25.85579296540004	26.8760393723348	21.22283847477002	26.045329187495135
16	26.019138727714726	25.31032248726661	23.117560703282766	25.552978081735894
17	24.59244789230798	25.524966105015867	24.52101489016253	25.361571112513627
18	25.15682276246336	24.495267502070128	25.30446409494731	25.043445640519202
19	25.457274597124762	24.494332128506542	25.024393557934577	25.02399971643412
20	25.473766709956415	24.397496349581594	25.007852214915367	25.120884725546627
21	25.82901174336894	24.279639280569732	24.820777502198126	25.0705714738632
22	25.57567319819975	24.20264326723032	25.036602644448752	25.185080890121174
23	25.175333312984854	24.20087098047826	25.141906015633538	25.48188969090334
24	25.220378934599662	24.606970797637345	24.847263343103883	25.325386924659103
25	25.79991670252265	24.28978069920651	24.726058621338133	25.184243976932702
26	25.461360702692005	24.82087596257324	24.645764185432395	25.07199914930236
27	25.49311417366638	24.405323949403183	24.605641582573302	25.495920294357138
28	25.23928332662162	24.5208179694123	24.900481175853184	25.3394175281129
29	24.837564996155123	24.74456917185963	25.187345478748806	25.23052035323644
30	24.989833966269444	24.972504940249323	24.927754699759856	25.109906393721378
31	25.47002521570207	24.654477928630012	24.661911686951147	25.213585168716772
32	25.025624312623506	24.63572122717073	24.767805820386616	25.57084863981915
33	24.83692500371688	24.394050236452593	25.226188096731413	25.542836663099116
34	25.491981879352565	24.214507742431596	24.92662240544604	25.366887972769796
35	25.257350805455097	24.397151738268693	25.427539563840227	24.91795789243598
36	25.021981278744278	24.640693476114006	24.92401320550551	25.41331203963621
37	25.22170814966371	24.123038053950378	25.292353468808244	25.36290032757767
38	24.899791953227382	24.223763017692345	25.599992910852993	25.276452118227283
39	24.831411222710475	24.138791713968672	25.56819020969106	25.461606853629792
40	24.858586286242033	24.012860894197434	25.968185483593054	25.160367335967475
41	24.40340397208845	23.972196759275214	26.320279785001926	25.30411948363441
42	24.614650706896263	24.154053072111395	26.088208680857434	25.14308754013491
43	24.387404161132373	23.61478559761017	26.320427475564596	25.67738276569286
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	147.0
1	171.0
2	195.0
3	387.0
4	579.0
5	579.0
6	755.5
7	932.0
8	841.0
9	750.0
10	1125.5
11	1501.0
12	1501.0
13	2584.5
14	3668.0
15	5103.5
16	6539.0
17	6865.0
18	7191.0
19	7191.0
20	9557.0
21	11923.0
22	12591.5
23	13260.0
24	16260.0
25	19260.0
26	19260.0
27	22602.5
28	25945.0
29	30802.5
30	35660.0
31	41053.0
32	46446.0
33	46446.0
34	54313.0
35	62180.0
36	70558.5
37	78937.0
38	86719.5
39	94502.0
40	94502.0
41	102908.5
42	111315.0
43	115734.0
44	120153.0
45	126695.5
46	133238.0
47	133238.0
48	138620.5
49	144003.0
50	147523.5
51	151044.0
52	151582.5
53	152121.0
54	152121.0
55	150360.5
56	148600.0
57	144202.0
58	139804.0
59	134737.5
60	129671.0
61	129671.0
62	119467.0
63	109263.0
64	98330.5
65	87398.0
66	76943.0
67	66488.0
68	66488.0
69	57227.0
70	47966.0
71	40627.0
72	33288.0
73	26847.0
74	20406.0
75	20406.0
76	16093.5
77	11781.0
78	9245.0
79	6709.0
80	5215.0
81	3721.0
82	3721.0
83	2789.5
84	1858.0
85	1553.5
86	1249.0
87	1042.0
88	835.0
89	835.0
90	712.5
91	590.0
92	345.5
93	101.0
94	74.0
95	47.0
96	47.0
97	28.5
98	10.0
99	5.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2031274.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.62405906117252
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.50588980768067	49.19336046787745
2	9.556150539764264	11.3955296836072
3	2.987873240362974	5.3444739165209665
4	1.3912210706102106	3.318009891248434
5	0.8343656779546755	2.487413423049241
6	0.540117483241402	1.9322398032454324
7	0.36980595088060775	1.5434532299534893
8	0.2673313044281908	1.275150198730139
9	0.21518141670384158	1.1546990548574962
>10	1.2077548336141557	13.185304856908198
>50	0.08066048067177076	3.285040016856415
>100	0.041251793842842416	4.680820842299994
>500	0.0019832277885040617	0.7538315788087189
>1k	4.1317245593834254E-4	0.45067303603681713
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2652	0.130558457401611	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2321	0.11426326532018821	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.9230187557168555E-5	4.9230187557168555E-5	0.0	0.0	0.0
6	4.9230187557168555E-5	4.9230187557168555E-5	0.0	0.0	0.0
7	4.9230187557168555E-5	4.9230187557168555E-5	0.0	0.0	0.0
8	4.9230187557168555E-5	4.9230187557168555E-5	0.0	0.0	0.0
9	4.9230187557168555E-5	4.9230187557168555E-5	0.0	4.9230187557168555E-5	0.0
10	4.9230187557168555E-5	4.9230187557168555E-5	0.0	9.846037511433711E-5	0.0
11	9.846037511433711E-5	4.9230187557168555E-5	0.0	9.846037511433711E-5	0.0
12	9.846037511433711E-5	4.9230187557168555E-5	0.0	9.846037511433711E-5	0.0
13	9.846037511433711E-5	4.9230187557168555E-5	0.0	9.846037511433711E-5	0.0
14	9.846037511433711E-5	4.9230187557168555E-5	0.0	9.846037511433711E-5	0.0
15	9.846037511433711E-5	4.9230187557168555E-5	0.0	1.9692075022867422E-4	0.0
16	9.846037511433711E-5	4.9230187557168555E-5	0.0	3.9384150045734844E-4	0.0
17	9.846037511433711E-5	4.9230187557168555E-5	0.0	4.923018755716856E-4	0.0
18	9.846037511433711E-5	4.9230187557168555E-5	0.0	5.907622506860227E-4	0.0
19	9.846037511433711E-5	4.9230187557168555E-5	0.0	6.892226258003597E-4	0.0
20	9.846037511433711E-5	4.9230187557168555E-5	0.0	9.353735635862025E-4	0.0
21	9.846037511433711E-5	4.9230187557168555E-5	0.0	0.0013784452516007195	0.0
22	9.846037511433711E-5	4.9230187557168555E-5	0.0	0.0019692075022867423	0.0
23	9.846037511433711E-5	4.9230187557168555E-5	0.0	0.002559969752972765	0.0
24	9.846037511433711E-5	4.9230187557168555E-5	0.0	0.003839954629459147	0.0
25	9.846037511433711E-5	4.9230187557168555E-5	0.0	0.004923018755716856	0.0
26	9.846037511433711E-5	4.9230187557168555E-5	0.0	0.0061537734446460695	0.0
27	1.4769056267150568E-4	4.9230187557168555E-5	0.0	0.014079833641350207	0.0
28	1.4769056267150568E-4	4.9230187557168555E-5	0.0	0.032590384162845584	0.0
29	1.4769056267150568E-4	4.9230187557168555E-5	0.0	0.06025774956997431	0.0
30	1.4769056267150568E-4	4.9230187557168555E-5	0.0	0.1034818542451683	0.0
31	1.4769056267150568E-4	4.9230187557168555E-5	0.0	0.21922202519207157	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1165	0.0	19.055796	1
CTGCGTA	65	0.0015804986	17.076923	9
TCCTATA	125	1.6594277E-7	16.279999	2
TCTAGCG	230	0.0	16.086956	28
GACGGAC	410	0.0	15.792684	7
AAGACGG	505	0.0	15.752476	5
TATACTT	235	0.0	15.744681	5
ATACCGC	305	0.0	15.163935	27
GTCTTAC	210	9.094947E-12	14.9761915	1
CTAGCGG	250	0.0	14.799999	29
ACGGACC	440	0.0	14.715909	8
CTATACT	290	0.0	14.672415	4
CGGGTAA	240	0.0	14.645833	24
TATACTG	295	0.0	14.42373	5
CGGACCA	450	0.0	14.388889	9
CGTTATT	220	1.8189894E-11	14.295454	2
CGCAATA	260	0.0	14.230769	36
TACACCG	130	4.4477183E-6	14.230769	5
TACCGCA	330	0.0	14.015151	28
ATACCGT	315	0.0	13.507937	6
>>END_MODULE