FastQCFastQC Report
Fri 10 Feb 2017
ERR1630300.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630300.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126444
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT14870.13200833774248874No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA12550.11141255135630355No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT11650.1034228066375248No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC11530.10235750734168764No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA500.007034795418.56
AAGACGG4100.018.048785
GACGGAC4300.017.6395347
CGCGAAC752.0672208E-417.26666628
CGCAAGA4550.017.0769232
CAAGACG4450.017.0449454
CTTATAC2400.016.95833237
TAACACT1107.807266E-716.8181824
GCGAACT803.3824897E-416.187529
GTGTAAC1151.2420642E-616.0869561
GCGCAAG4950.016.0707071
GTATAGA1059.342686E-615.8571421
TAAACGC2700.015.75925828
GGTATCA6350.015.7322841
TTTACAC957.0594746E-515.5789483
GTTCAAA4550.015.4505491
TCTTATA4200.015.41666637
TTTCGGA2650.015.35849130
CGGACCA5300.015.3584919
ACGGACC4950.015.3232338
Produced by FastQC (version 0.11.5)