FastQCFastQC Report
Fri 10 Feb 2017
ERR1630299.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630299.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162888
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT98610.8479750414485315No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT78070.6713458217816333No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64890.5580073059486382No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT47650.4097557116420498No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT33010.28386224640722063No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30750.2644278726756145No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA29910.2572044771293538No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC26980.23200858552156356No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC26950.2317506071091971No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG26020.22375327632583705No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC25820.22203342024339404No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA25400.21842172247026367No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG24700.21240222618171312No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC24590.21145630533636944No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG24350.20939247803743785No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG24330.20922049242919355No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT23460.2017391184705664No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG23220.19967529117163474No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG23110.19872937032629107No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA21930.18858221943987727No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA21280.18299268717193745No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC20530.17654322686277613No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC20510.17637124125453182No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA20340.17490936358445525No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG20330.1748233707803331No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG19990.17189961544017998No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC18760.1613225005331554No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC18440.15857073080124656No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC18080.15547498985284913No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG17540.15083137843025296No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAG16940.14567181018292388No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC16860.14498386774994668No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT16640.14309202605925936No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGC16280.13999628511086193No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG16060.1381044434201746No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGG16000.13758848659544168No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC15950.13715852257483094No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGG15860.1363845873377316No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG15800.1358686305129987No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTT15590.1340627816264335No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC15280.13139700469864682No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACT15270.13131101189452465No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG15210.13079505506979175No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA15170.13045108385330315No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC15080.12967714861620377No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCG14830.12752732851315002No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTA14530.12494754438948548No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTT14460.12434559476063042No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG14150.12167981783284376No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGA13840.11901404090505707No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC13760.11832609847207987No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTG13600.11695021360612545No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG13390.11514436471956027No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA13260.1140264582659723No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT13130.11290855181238435No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAG12850.11050075329696411No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG12720.10938284684337614No Hit
TCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTC12420.1068030627197116No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCT12410.10671706991558948No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGA12380.10645909150322301No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGG12380.10645909150322301No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG12350.10620111309085656No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGG11880.10215945129711546No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGT11880.10215945129711546No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTC11840.10181548008062685No Hit
CCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAAT11720.10078356643116104No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC11710.10069757362703889No Hit
GGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTTAATGA11650.10018161680230599No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCTA200.001841944637.04
GTAAACA555.002221E-1033.63636436
AATACTG754.620233E-1027.1333335
ATCGTGG358.869461E-426.4285734
CACCGTC706.5920176E-926.428577
AAGACGG8750.024.7371435
TGCTAGA751.3737008E-824.66666616
TACACAG451.3232254E-424.6666665
ACCGTCC701.9229628E-723.7857138
CAAGACG9850.022.9137064
CTCTGAT652.6828911E-622.769237
CATAGCA502.702128E-422.218
ACGTGAT502.702128E-422.26
TACGTGA502.702128E-422.25
GCGCAAG9750.022.1999991
CGCAAGA9750.022.1999992
GACGGAC9950.021.93977
ATCTTAT603.726335E-521.5833327
AGACGGA10050.021.5373136
GCAAGAC10500.021.3190463
Produced by FastQC (version 0.11.5)