##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630299.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1162888
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.760711263681454	31.0	31.0	34.0	30.0	34.0
2	31.93124101375197	33.0	31.0	34.0	30.0	34.0
3	32.04942608402529	33.0	31.0	34.0	30.0	34.0
4	35.73082962417705	37.0	35.0	37.0	33.0	37.0
5	35.659588885602055	37.0	35.0	37.0	33.0	37.0
6	35.72459772566231	37.0	35.0	37.0	35.0	37.0
7	35.69332902222742	37.0	35.0	37.0	33.0	37.0
8	35.69044310372108	37.0	35.0	37.0	33.0	37.0
9	37.36031844855223	39.0	37.0	39.0	34.0	39.0
10	37.26540647078652	39.0	37.0	39.0	34.0	39.0
11	37.3332943499288	39.0	37.0	39.0	34.0	39.0
12	37.203913876486816	39.0	37.0	39.0	34.0	39.0
13	37.24978587791774	39.0	37.0	39.0	34.0	39.0
14	38.34941972055778	40.0	38.0	41.0	34.0	41.0
15	38.31576213702437	40.0	38.0	41.0	34.0	41.0
16	38.28280367498848	40.0	38.0	41.0	33.0	41.0
17	38.284152902085154	40.0	38.0	41.0	33.0	41.0
18	38.25048327955917	40.0	38.0	41.0	33.0	41.0
19	38.22595727189549	40.0	38.0	41.0	33.0	41.0
20	38.18699823198795	40.0	38.0	41.0	33.0	41.0
21	38.103580052421215	40.0	38.0	41.0	33.0	41.0
22	38.050426180337226	40.0	37.0	41.0	33.0	41.0
23	37.93474952016015	40.0	37.0	41.0	33.0	41.0
24	37.85620455280302	40.0	37.0	41.0	33.0	41.0
25	37.82655165415758	40.0	37.0	41.0	33.0	41.0
26	37.59433840576221	40.0	37.0	41.0	32.0	41.0
27	37.44549776074738	40.0	37.0	41.0	32.0	41.0
28	37.21879235145603	39.0	36.0	41.0	31.0	41.0
29	37.10431959053666	39.0	36.0	41.0	31.0	41.0
30	36.96677496027132	39.0	36.0	41.0	31.0	41.0
31	36.81063352618653	39.0	35.0	40.0	31.0	41.0
32	36.64947183219708	39.0	35.0	40.0	30.0	41.0
33	36.46613689366474	39.0	35.0	40.0	30.0	41.0
34	36.36368678668969	39.0	35.0	40.0	30.0	41.0
35	36.1424316013236	38.0	35.0	40.0	29.0	41.0
36	35.95930648523331	38.0	35.0	40.0	29.0	41.0
37	35.85123846836497	38.0	35.0	40.0	28.0	41.0
38	35.63157586973122	38.0	35.0	40.0	27.0	41.0
39	35.49159764310922	38.0	34.0	40.0	27.0	41.0
40	35.24677612977346	38.0	34.0	40.0	26.0	41.0
41	35.03034686057471	38.0	34.0	40.0	25.0	41.0
42	34.80418664566149	38.0	33.0	40.0	24.0	41.0
43	33.471181231554546	36.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	12.0
11	10.0
12	9.0
13	11.0
14	12.0
15	14.0
16	46.0
17	109.0
18	185.0
19	319.0
20	591.0
21	1017.0
22	1681.0
23	2641.0
24	3956.0
25	5673.0
26	7904.0
27	10617.0
28	14239.0
29	18701.0
30	24319.0
31	30772.0
32	38331.0
33	48768.0
34	64430.0
35	84278.0
36	118534.0
37	177411.0
38	263986.0
39	244304.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.813333700235965	19.36953515729804	12.234798192087286	24.582332950378714
2	20.687460873274123	22.036687969950673	30.494682204993083	26.781168951782114
3	20.555203940534255	23.504241165099305	29.406615254435508	26.53393963993093
4	13.813024126141125	15.695492601179135	33.99235351985746	36.499129752822284
5	15.265786558980746	36.566032154429315	31.68740239816732	16.480778888422616
6	34.09640481284526	34.992621817406324	15.710455349096389	15.200518020652032
7	29.337649025529544	30.943908613727206	20.511949560060813	19.20649280068244
8	29.975457653703536	29.246582645964185	19.542724664800048	21.235235035532227
9	27.87327756413343	12.60233143690536	19.952308390833856	39.57208260812735
10	19.94095734068973	24.44594836304098	28.333511051795185	27.279583244474104
11	38.92653462758236	20.92643487592958	19.370653063751625	20.776377432736428
12	23.444734144646777	26.30648867302784	25.341219446756696	24.907557735568687
13	30.523059830353393	16.80669161604557	25.18884019785224	27.481408355748794
14	24.195021360612547	20.979234457660585	23.09517339589023	31.730570785836644
15	27.28543075515441	27.04404895398353	20.4594939495463	25.21102634131576
16	27.132535549425224	24.024755608450686	23.143329366198635	25.699379475925454
17	25.283604267994853	25.089260530678793	23.488160510728463	26.138974690597887
18	25.928206327694497	21.06754906749403	26.462909583726034	26.541335021085438
19	25.313185792612874	23.6016710121697	25.58002146380391	25.505121731413517
20	25.975932333982293	21.88688850516989	25.106201113090858	27.030978047756964
21	28.838030833579847	22.94322411100639	24.46951039137045	23.74923466404331
22	27.392921760307097	22.430191041613636	24.756382385921945	25.42050481215732
23	24.482237326380528	22.08131823529007	26.207252977070876	27.229191461258523
24	24.637884301841623	24.549741677616417	25.45859962438343	25.353774396158528
25	25.406917949106017	23.11065210063222	25.204576881006595	26.277853069255166
26	25.09106637956536	25.736872338522716	25.2127461973982	23.959315084513726
27	26.67376393943355	23.100934913766416	24.796713011055235	25.4285881357448
28	23.997753867956327	23.908579330081658	27.238134712887224	24.855532089074785
29	23.631338529591844	24.451795873721288	26.79578772848288	25.12107786820399
30	23.010986440654648	25.736958331326836	27.190752677815922	24.061302550202598
31	25.416721128775944	25.14833758711071	24.06861193855298	25.366329345560363
32	24.006181162760303	23.784233735321028	24.05407915465634	28.155505947262334
33	23.002387160242428	23.47895928068739	26.339337924202503	27.179315634867674
34	25.766969819965464	23.631424522395967	25.623447829885592	24.978157827752973
35	26.22436554509119	23.893186618143794	26.617266667125293	23.265181169639725
36	22.95354324750105	25.857778221118455	25.562048967742378	25.626629563638115
37	24.8264665212815	24.5842247920694	25.81761958159341	24.77168910505569
38	23.69720901754941	23.41721644732769	27.5330900310262	25.352484504096694
39	25.112650573400018	22.767110848164226	26.0963222597533	26.023916318682456
40	24.39349275252647	24.263729611106143	27.285602740762656	24.057174895604735
41	22.900743665770047	24.116337944840776	28.30969104505335	24.673227344335828
42	24.914265174290215	24.493674369328776	27.64918031659111	22.942880139789903
43	23.335953247432258	22.57930256396145	27.894861757968094	26.189882430638207
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	461.0
1	362.0
2	263.0
3	596.0
4	929.0
5	929.0
6	1319.0
7	1709.0
8	1651.0
9	1593.0
10	2151.5
11	2710.0
12	2710.0
13	5232.5
14	7755.0
15	12969.5
16	18184.0
17	15412.0
18	12640.0
19	12640.0
20	13496.0
21	14352.0
22	10165.5
23	5979.0
24	6168.5
25	6358.0
26	6358.0
27	6927.5
28	7497.0
29	8363.0
30	9229.0
31	10956.0
32	12683.0
33	12683.0
34	17244.5
35	21806.0
36	29159.0
37	36512.0
38	43251.0
39	49990.0
40	49990.0
41	55633.0
42	61276.0
43	62301.5
44	63327.0
45	70070.5
46	76814.0
47	76814.0
48	84659.5
49	92505.0
50	88541.0
51	84577.0
52	86116.0
53	87655.0
54	87655.0
55	87298.5
56	86942.0
57	86478.0
58	86014.0
59	83928.5
60	81843.0
61	81843.0
62	73339.5
63	64836.0
64	57034.5
65	49233.0
66	42701.5
67	36170.0
68	36170.0
69	31346.0
70	26522.0
71	23896.0
72	21270.0
73	16293.0
74	11316.0
75	11316.0
76	8232.5
77	5149.0
78	4541.0
79	3933.0
80	3793.0
81	3653.0
82	3653.0
83	3047.5
84	2442.0
85	2422.0
86	2402.0
87	2319.0
88	2236.0
89	2236.0
90	1991.0
91	1746.0
92	991.5
93	237.0
94	169.0
95	101.0
96	101.0
97	62.5
98	24.0
99	19.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1162888.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.998179704030743
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.56147381677	14.310992132730574
2	11.606228545713462	4.642068882864584
3	4.647361433069078	2.788163072642918
4	2.4719234121124734	1.9773587444010434
5	1.5851675077760041	1.5850232340747537
6	1.0799737588501919	1.2958505583074218
7	0.8148465552193312	1.1406813489740617
8	0.6524392626534452	1.0438078096407128
9	0.5126451698211546	0.9226773207438128
>10	3.9346813732280985	15.854737572764247
>50	0.52618677254963	7.376355268576542
>100	0.48144153572883175	20.626106847110787
>500	0.08690901717356928	12.065229262070307
>1k	0.037431111356933305	12.29246275120669
>5k	0.0012907279778252862	2.0784851938915643
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9861	0.8479750414485315	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7807	0.6713458217816333	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6489	0.5580073059486382	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	4765	0.4097557116420498	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	3301	0.28386224640722063	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3075	0.2644278726756145	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2991	0.2572044771293538	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	2698	0.23200858552156356	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC	2695	0.2317506071091971	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG	2602	0.22375327632583705	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	2582	0.22203342024339404	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	2540	0.21842172247026367	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG	2470	0.21240222618171312	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC	2459	0.21145630533636944	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG	2435	0.20939247803743785	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG	2433	0.20922049242919355	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	2346	0.2017391184705664	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG	2322	0.19967529117163474	No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG	2311	0.19872937032629107	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA	2193	0.18858221943987727	No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA	2128	0.18299268717193745	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2053	0.17654322686277613	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2051	0.17637124125453182	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA	2034	0.17490936358445525	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG	2033	0.1748233707803331	No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG	1999	0.17189961544017998	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	1876	0.1613225005331554	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC	1844	0.15857073080124656	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC	1808	0.15547498985284913	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1754	0.15083137843025296	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAG	1694	0.14567181018292388	No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	1686	0.14498386774994668	No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT	1664	0.14309202605925936	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGC	1628	0.13999628511086193	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG	1606	0.1381044434201746	No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGG	1600	0.13758848659544168	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1595	0.13715852257483094	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGG	1586	0.1363845873377316	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG	1580	0.1358686305129987	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTT	1559	0.1340627816264335	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1528	0.13139700469864682	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACT	1527	0.13131101189452465	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG	1521	0.13079505506979175	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA	1517	0.13045108385330315	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1508	0.12967714861620377	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCG	1483	0.12752732851315002	No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTA	1453	0.12494754438948548	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTT	1446	0.12434559476063042	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	1415	0.12167981783284376	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGA	1384	0.11901404090505707	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1376	0.11832609847207987	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTG	1360	0.11695021360612545	No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG	1339	0.11514436471956027	No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA	1326	0.1140264582659723	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT	1313	0.11290855181238435	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAG	1285	0.11050075329696411	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	1272	0.10938284684337614	No Hit
TCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTC	1242	0.1068030627197116	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCT	1241	0.10671706991558948	No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGA	1238	0.10645909150322301	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGG	1238	0.10645909150322301	No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG	1235	0.10620111309085656	No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGG	1188	0.10215945129711546	No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGT	1188	0.10215945129711546	No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTC	1184	0.10181548008062685	No Hit
CCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAAT	1172	0.10078356643116104	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	1171	0.10069757362703889	No Hit
GGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTTAATGA	1165	0.10018161680230599	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	8.599280412215105E-5	0.0
6	0.0	0.0	0.0	8.599280412215105E-5	0.0
7	0.0	0.0	0.0	8.599280412215105E-5	0.0
8	0.0	0.0	0.0	8.599280412215105E-5	0.0
9	0.0	0.0	0.0	8.599280412215105E-5	0.0
10	0.0	0.0	0.0	8.599280412215105E-5	0.0
11	0.0	0.0	0.0	1.719856082443021E-4	0.0
12	0.0	0.0	0.0	1.719856082443021E-4	0.0
13	0.0	0.0	0.0	1.719856082443021E-4	0.0
14	0.0	0.0	0.0	1.719856082443021E-4	0.0
15	0.0	0.0	0.0	3.439712164886042E-4	0.0
16	0.0	0.0	0.0	0.0010319136494658127	0.0
17	0.0	0.0	0.0	0.0023218057112980787	0.0
18	0.0	0.0	0.0	0.0027517697319088337	0.0
19	0.0	0.0	0.0	0.002923755340153136	0.0
20	0.0	0.0	0.0	0.003181733752519589	0.0
21	0.0	0.0	0.0	0.004557618618474006	0.0
22	0.0	0.0	0.0	0.007137402742138538	0.0
23	0.0	0.0	0.0	0.011523035752368242	0.0
24	0.0	0.0	0.0	0.01711256802030806	0.0
25	0.0	0.0	0.0	0.01857444569038463	0.0
26	0.0	0.0	0.0	0.024163977958324447	0.0
27	0.0	0.0	0.0	0.040072646720922395	0.0
28	0.0	0.0	0.0	0.07962933661711188	0.0
29	0.0	0.0	0.0	0.14446791092521377	0.0
30	0.0	0.0	0.0	0.23708216096477047	0.0
31	0.0	0.0	0.0	0.48620331450664206	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGCTA	20	0.0018419446	37.0	4
GTAAACA	55	5.002221E-10	33.636364	36
AATACTG	75	4.620233E-10	27.133333	5
ATCGTGG	35	8.869461E-4	26.42857	34
CACCGTC	70	6.5920176E-9	26.42857	7
AAGACGG	875	0.0	24.737143	5
TGCTAGA	75	1.3737008E-8	24.666666	16
TACACAG	45	1.3232254E-4	24.666666	5
ACCGTCC	70	1.9229628E-7	23.785713	8
CAAGACG	985	0.0	22.913706	4
CTCTGAT	65	2.6828911E-6	22.76923	7
CATAGCA	50	2.702128E-4	22.2	18
ACGTGAT	50	2.702128E-4	22.2	6
TACGTGA	50	2.702128E-4	22.2	5
GCGCAAG	975	0.0	22.199999	1
CGCAAGA	975	0.0	22.199999	2
GACGGAC	995	0.0	21.9397	7
ATCTTAT	60	3.726335E-5	21.583332	7
AGACGGA	1005	0.0	21.537313	6
GCAAGAC	1050	0.0	21.319046	3
>>END_MODULE