FastQCFastQC Report
Fri 10 Feb 2017
ERR1630298.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630298.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012960
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT63800.6298373084820724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46950.4634931290475438No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41750.4121584267888169No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19270.19023456010108988No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT15570.15370794503238033No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA11750.11599668298846944No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT11270.11125809508766386No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10510.10375533091138842No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACGA200.001841832437.05
AGTCCGC358.8686636E-426.42857210
TGCCGAA451.323061E-424.66666612
TTAACGG502.701796E-422.19999935
CTATAGA555.1425153E-420.1818181
TACACCG555.1425153E-420.1818185
TGTGCGC656.9010974E-519.92307710
TATACAG759.263991E-619.7333345
CGCAAGA3850.019.7012982
TCTTATA8600.019.5755837
CGGACCA3750.018.7466689
GCGCAAG4150.018.722891
ACGGACC3800.018.58
GACGGAC3800.018.57
TAGAGTA500.00703432118.4999984
CAAGACG4350.018.2873554
AAGACGG4100.018.0487825
CAGTCGG1552.0008883E-1117.90322510
CTTATAC4350.017.86206837
TTAGGAC2200.017.659093
Produced by FastQC (version 0.11.5)