FastQCFastQC Report
Fri 10 Feb 2017
ERR1630297.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630297.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2707728
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG97700.3608191073844936No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT72040.2660533111154444No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG64280.23739459798029935No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC57170.2111364213835363No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA55650.20552285901685843No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT45220.16700348040866736No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC44750.16526770783476036No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC42460.1568104329533838No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT41840.15452069040908098No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA40480.14949802934415865No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC40400.14920257869328085No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC39830.14709749280577666No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC39010.14406912363427937No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC37780.13952656987703344No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA36830.13601809339785975No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC36370.13431925215531249No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36220.13376528218491665No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT35090.12959204174126795No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA34740.12829944514367767No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA32730.12087624754037332No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG32590.12035920890133721No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32000.11818026035111356No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG30950.11430247055834264No Hit
GTGTTCATCTCATCAGAGAAAGAACCATCAGCATGTCTGCGGC30670.1132683932802704No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA30090.11112637606140648No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA29520.10902129017390226No Hit
GCCTTGTACCAGCATTACAAATAATCCAGCCACAAAGTAAATG29500.1089474275111828No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC29410.1086150455289453No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG29180.10776562490767166No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA27440.10133957325107988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12400.022.3790321
CGGCCTT13300.018.08270624
TAGTACG953.6100446E-617.5263164
GGATACG650.001580682117.0769231
TCGCCAT14000.016.91428613
TTCCTCG14500.016.58620619
TCTCGCC15200.016.0657912
ATCTCGC15250.015.77049211
TCGGCCT15700.015.67197423
GTCGCCA15400.015.61688212
AAGACCG957.06533E-515.5789485
TTGGCCG15550.015.46623931
GCTTAGG26300.015.4049431
CGCAAGA3750.015.2933342
CTCGCCT16150.015.23529413
TCCTCGG16050.015.21495320
CAAGACG4500.015.2111114
GACGGAC3550.015.1126777
CGAATTA1353.9776387E-715.07407515
AAGACGG4050.015.0740745
Produced by FastQC (version 0.11.5)