##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630297.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2707728
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98816683211903	33.0	31.0	34.0	30.0	34.0
2	32.186150529152115	33.0	31.0	34.0	30.0	34.0
3	32.333867360384794	34.0	31.0	34.0	30.0	34.0
4	35.95260528383944	37.0	35.0	37.0	35.0	37.0
5	35.87037841319364	37.0	35.0	37.0	35.0	37.0
6	35.935059208310435	37.0	35.0	37.0	35.0	37.0
7	35.918947545691445	37.0	35.0	37.0	35.0	37.0
8	35.899790156175214	37.0	35.0	37.0	35.0	37.0
9	37.62946906040784	39.0	37.0	39.0	35.0	39.0
10	37.561283851258324	39.0	37.0	39.0	35.0	39.0
11	37.62871750781467	39.0	37.0	39.0	35.0	39.0
12	37.52740046267572	39.0	37.0	39.0	35.0	39.0
13	37.55247942186217	39.0	37.0	39.0	35.0	39.0
14	38.82882697228082	40.0	38.0	41.0	35.0	41.0
15	38.84637267849651	40.0	38.0	41.0	35.0	41.0
16	38.822385409465056	40.0	38.0	41.0	35.0	41.0
17	38.792357282563096	40.0	38.0	41.0	35.0	41.0
18	38.756924624629946	40.0	38.0	41.0	35.0	41.0
19	38.793792064786416	40.0	38.0	41.0	35.0	41.0
20	38.74926912895239	40.0	38.0	41.0	35.0	41.0
21	38.70894380824071	40.0	38.0	41.0	34.0	41.0
22	38.703905636016614	40.0	38.0	41.0	34.0	41.0
23	38.6600648218728	40.0	38.0	41.0	34.0	41.0
24	38.65865367570155	40.0	38.0	41.0	34.0	41.0
25	38.628358535273854	40.0	38.0	41.0	34.0	41.0
26	38.499981165021005	40.0	38.0	41.0	34.0	41.0
27	38.4186746231527	40.0	38.0	41.0	34.0	41.0
28	38.3677263742887	40.0	38.0	41.0	34.0	41.0
29	38.32602499217056	40.0	38.0	41.0	34.0	41.0
30	38.28275292052968	40.0	38.0	41.0	34.0	41.0
31	38.193883949938844	40.0	38.0	41.0	34.0	41.0
32	38.10814638693399	40.0	38.0	41.0	33.0	41.0
33	38.076549047762555	40.0	38.0	41.0	33.0	41.0
34	38.00173946570704	40.0	38.0	41.0	33.0	41.0
35	37.938277404525124	40.0	37.0	41.0	33.0	41.0
36	37.857351993996446	40.0	37.0	41.0	33.0	41.0
37	37.81967945081633	40.0	37.0	41.0	33.0	41.0
38	37.732261512234615	40.0	37.0	41.0	33.0	41.0
39	37.655896013188915	40.0	37.0	41.0	33.0	41.0
40	37.597547833460375	40.0	37.0	41.0	32.0	41.0
41	37.502078495328924	40.0	37.0	41.0	32.0	41.0
42	37.474433547239606	40.0	37.0	41.0	32.0	41.0
43	36.34466534304775	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	4.0
12	2.0
13	0.0
14	2.0
15	4.0
16	14.0
17	39.0
18	67.0
19	126.0
20	273.0
21	530.0
22	964.0
23	1702.0
24	2783.0
25	4728.0
26	7344.0
27	10672.0
28	16127.0
29	23232.0
30	32269.0
31	44223.0
32	59143.0
33	79054.0
34	107961.0
35	149119.0
36	215302.0
37	333113.0
38	645906.0
39	973022.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.340848120638405	19.170352413536367	12.53851937860819	25.95028008721703
2	17.18765695815828	21.204862526812146	36.567594677161075	25.039885837868503
3	19.215741019777465	23.106641435181082	31.523365714724672	26.15425183031678
4	14.584367410611407	15.468355758037736	35.18835717620086	34.75891965515
5	13.560261592006286	37.69809966141355	35.133255629812155	13.608383116768005
6	34.17038195860145	35.44137372734632	16.06752967801788	14.320714636034342
7	28.46859064130518	31.733135676847894	21.19788250518516	18.600391176661763
8	25.88073839026667	35.92550655014093	20.18068284554431	18.013072214048087
9	25.882621888166017	15.244182576684217	19.498967399975182	39.37422813517458
10	16.188258200232816	28.105518722707746	33.70091087435665	22.005312202702783
11	34.737277894973204	22.342236738697537	22.196284117163913	20.724201249165354
12	20.300746603794767	26.210424385314923	29.656006807182994	23.832822203707316
13	28.998887628299446	20.12015239344572	26.25607151087554	24.624888467379293
14	22.012846194300167	21.35376965485455	25.096427706180236	31.536956444665048
15	24.49983897939527	28.712337428279355	23.003122913379777	23.784700678945594
16	24.508813292915686	27.89168631413495	23.692372350546286	23.907128042403077
17	23.356740411149126	27.685387897159536	25.437931727263596	23.519939964427742
18	22.08615488704922	26.51285505781969	27.25853556930386	24.142454485827233
19	23.989817293317497	26.711582551866364	27.004558803543045	22.294041351273098
20	24.022575384233573	25.626872418499936	27.456524436723335	22.894027760543157
21	23.13467231568311	27.14563648933719	25.76643591970833	23.95325527527137
22	23.531351745817897	26.18778547919141	26.07824715037847	24.20261562461222
23	22.806057329244297	26.992223738868898	26.369524560812607	23.832194371074202
24	24.64431434767451	26.62128544669184	26.060593973988528	22.673806231645127
25	24.213658092688778	25.854997252308948	26.381527243504515	23.54981741149776
26	24.073873003492228	26.57046793474086	25.89720976405311	23.458449297713802
27	24.02763497662985	25.825784569203407	25.980637641594722	24.165942812572016
28	23.05349724935444	26.356561663505346	26.75597401216075	23.83396707497947
29	23.253258820679182	27.068486938126725	26.162856830523594	23.515397410670495
30	22.86821275992271	27.85143116295285	26.0717103047278	23.208645772396636
31	23.817126387879433	26.44759739530706	26.421745463355258	23.31353075345825
32	22.743495653920924	26.78640542920116	26.451696773087992	24.01840214378992
33	23.118385598553473	26.265710588360424	26.569175338143268	24.04672847494283
34	23.309689894996836	26.263125395165243	26.595876690716352	23.831308019121565
35	23.964925575981045	26.193140522238572	26.44438436947877	23.39754953230162
36	23.349169488220383	26.839217233045567	26.47034709542465	23.341266183309404
37	24.639771793917262	25.193704832981744	26.17364077928064	23.992882593820354
38	23.669918101079578	26.076991485112245	27.134557089929267	23.11853332387891
39	23.666114173949527	25.852338196451047	27.08806054374738	23.39348708585205
40	23.310317727629954	24.869595468968818	28.085945117087093	23.734141686314132
41	22.55887592845367	26.01734738496629	27.98139251800772	23.44238416857232
42	23.640963937293556	24.766003084504796	27.552028859619575	24.041004118582073
43	22.724402155607947	25.135205604107945	27.642879934764498	24.49751230551961
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	201.0
1	189.0
2	177.0
3	389.0
4	601.0
5	601.0
6	851.0
7	1101.0
8	1060.5
9	1020.0
10	1794.0
11	2568.0
12	2568.0
13	3658.5
14	4749.0
15	8493.0
16	12237.0
17	13476.0
18	14715.0
19	14715.0
20	18017.0
21	21319.0
22	26480.0
23	31641.0
24	36039.0
25	40437.0
26	40437.0
27	45171.5
28	49906.0
29	68094.5
30	86283.0
31	89168.0
32	92053.0
33	92053.0
34	100678.5
35	109304.0
36	115094.0
37	120884.0
38	141663.0
39	162442.0
40	162442.0
41	176396.5
42	190351.0
43	174553.5
44	158756.0
45	172137.5
46	185519.0
47	185519.0
48	196947.0
49	208375.0
50	215114.0
51	221853.0
52	232460.5
53	243068.0
54	243068.0
55	204963.5
56	166859.0
57	155105.5
58	143352.0
59	127145.0
60	110938.0
61	110938.0
62	102567.0
63	94196.0
64	83724.0
65	73252.0
66	63904.5
67	54557.0
68	54557.0
69	46626.5
70	38696.0
71	33094.0
72	27492.0
73	22321.0
74	17150.0
75	17150.0
76	13362.5
77	9575.0
78	7916.0
79	6257.0
80	4565.0
81	2873.0
82	2873.0
83	2063.0
84	1253.0
85	1019.0
86	785.0
87	645.0
88	505.0
89	505.0
90	432.5
91	360.0
92	200.0
93	40.0
94	30.0
95	20.0
96	20.0
97	13.0
98	6.0
99	4.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2707728.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.130853430870296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.39805747152906	37.18694654736006
2	10.482531144782156	9.461711533593933
3	2.8368296854045862	3.8408563422100888
4	1.2289900894603931	2.2186148638171677
5	0.6872562652819454	1.5508230888943397
6	0.42885539377894716	1.16127659518055
7	0.29343821276361304	0.9270181879875808
8	0.21080866151470773	0.7611179843822578
9	0.15361641902589526	0.6239556082469553
>10	1.0148840841192917	8.712068550816317
>50	0.11396055672792194	3.5836263008581204
>100	0.11583046805948244	11.153528383818266
>500	0.02160320504397749	7.028544360723497
>1k	0.012929190897531943	10.509006464411872
>5k	4.0915161068139056E-4	1.280905187699119
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	9770	0.3608191073844936	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	7204	0.2660533111154444	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	6428	0.23739459798029935	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	5717	0.2111364213835363	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	5565	0.20552285901685843	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	4522	0.16700348040866736	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	4475	0.16526770783476036	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	4246	0.1568104329533838	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	4184	0.15452069040908098	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	4048	0.14949802934415865	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	4040	0.14920257869328085	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	3983	0.14709749280577666	No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC	3901	0.14406912363427937	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	3778	0.13952656987703344	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	3683	0.13601809339785975	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	3637	0.13431925215531249	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3622	0.13376528218491665	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	3509	0.12959204174126795	No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA	3474	0.12829944514367767	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	3273	0.12087624754037332	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	3259	0.12035920890133721	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3200	0.11818026035111356	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	3095	0.11430247055834264	No Hit
GTGTTCATCTCATCAGAGAAAGAACCATCAGCATGTCTGCGGC	3067	0.1132683932802704	No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA	3009	0.11112637606140648	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	2952	0.10902129017390226	No Hit
GCCTTGTACCAGCATTACAAATAATCCAGCCACAAAGTAAATG	2950	0.1089474275111828	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	2941	0.1086150455289453	No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG	2918	0.10776562490767166	No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA	2744	0.10133957325107988	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	3.693133135972299E-5	0.0	0.0	0.0
4	0.0	3.693133135972299E-5	0.0	0.0	0.0
5	0.0	3.693133135972299E-5	0.0	0.0	0.0
6	0.0	3.693133135972299E-5	0.0	0.0	0.0
7	0.0	3.693133135972299E-5	0.0	0.0	0.0
8	0.0	3.693133135972299E-5	0.0	0.0	0.0
9	0.0	7.386266271944598E-5	0.0	0.0	0.0
10	3.693133135972299E-5	7.386266271944598E-5	0.0	0.0	0.0
11	7.386266271944598E-5	1.8465665679861493E-4	0.0	0.0	0.0
12	7.386266271944598E-5	1.8465665679861493E-4	0.0	0.0	0.0
13	7.386266271944598E-5	1.8465665679861493E-4	0.0	0.0	0.0
14	7.386266271944598E-5	1.8465665679861493E-4	0.0	0.0	0.0
15	7.386266271944598E-5	1.8465665679861493E-4	0.0	3.693133135972299E-5	0.0
16	7.386266271944598E-5	1.8465665679861493E-4	0.0	7.386266271944598E-5	0.0
17	7.386266271944598E-5	1.8465665679861493E-4	0.0	1.1079399407916895E-4	0.0
18	7.386266271944598E-5	1.8465665679861493E-4	0.0	2.215879881583379E-4	0.0
19	7.386266271944598E-5	1.8465665679861493E-4	0.0	2.585193195180609E-4	0.0
20	7.386266271944598E-5	1.8465665679861493E-4	0.0	3.6931331359722986E-4	0.0
21	7.386266271944598E-5	1.8465665679861493E-4	0.0	5.170386390361218E-4	0.0
22	7.386266271944598E-5	1.8465665679861493E-4	0.0	8.124892899139057E-4	0.0
23	7.386266271944598E-5	2.215879881583379E-4	0.0	0.0013295279289500274	0.0
24	7.386266271944598E-5	2.215879881583379E-4	0.0	0.0021050858875042103	0.0
25	7.386266271944598E-5	2.215879881583379E-4	0.0	0.0030653005028570077	0.0
26	7.386266271944598E-5	2.215879881583379E-4	0.0	0.004025515118209805	0.0
27	7.386266271944598E-5	2.215879881583379E-4	0.0	0.0091589701772113	0.0
28	7.386266271944598E-5	2.215879881583379E-4	0.0	0.02548261863820886	0.0
29	7.386266271944598E-5	2.954506508777839E-4	0.0	0.04841697541259683	0.0
30	7.386266271944598E-5	2.954506508777839E-4	0.0	0.07988246973108082	0.0
31	7.386266271944598E-5	2.954506508777839E-4	0.0	0.17413122736109388	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1240	0.0	22.379032	1
CGGCCTT	1330	0.0	18.082706	24
TAGTACG	95	3.6100446E-6	17.526316	4
GGATACG	65	0.0015806821	17.076923	1
TCGCCAT	1400	0.0	16.914286	13
TTCCTCG	1450	0.0	16.586206	19
TCTCGCC	1520	0.0	16.06579	12
ATCTCGC	1525	0.0	15.770492	11
TCGGCCT	1570	0.0	15.671974	23
GTCGCCA	1540	0.0	15.616882	12
AAGACCG	95	7.06533E-5	15.578948	5
TTGGCCG	1555	0.0	15.466239	31
GCTTAGG	2630	0.0	15.404943	1
CGCAAGA	375	0.0	15.293334	2
CTCGCCT	1615	0.0	15.235294	13
TCCTCGG	1605	0.0	15.214953	20
CAAGACG	450	0.0	15.211111	4
GACGGAC	355	0.0	15.112677	7
CGAATTA	135	3.9776387E-7	15.074075	15
AAGACGG	405	0.0	15.074074	5
>>END_MODULE