FastQCFastQC Report
Fri 10 Feb 2017
ERR1630296.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630296.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216768
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24591.1343925302627695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23081.0647328018895779No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15790.7284285503395335No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7400.3413788012990847No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3900.1799158547387068No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2850.13147697077059345No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2800.12917035724830234No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2710.1250184529081783No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC2580.11902125775022145No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2570.11855993504576322No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.11717596693238855No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2440.11256273988780631No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCAA200.001838643537.010
CTGATCA200.001838643537.09
ACAAGCC200.001838643537.06
TACAAGC200.001838643537.05
CTTCTCG352.3794426E-531.71428510
AATCCGG250.005486629429.620
AATTTTC250.005486629429.615
GCGGTTC250.005486629429.616
ATCCGGG250.005486629429.621
TCAGAGT250.005486629429.613
TGAGCGT250.005486629429.627
TCGCGGT250.005486629429.614
GCATAAG405.9182563E-527.751
TCTTGCT405.9182563E-527.7517
TCGTCTT405.9182563E-527.7514
ATGATTT358.8461005E-426.4285722
AGGACGG358.8461005E-426.428575
GTCAGAT358.8461005E-426.4285713
TAGGGTC509.046726E-625.9000024
GTGTTTC451.3183789E-424.66666637
Produced by FastQC (version 0.11.5)