FastQCFastQC Report
Fri 10 Feb 2017
ERR1630295.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630295.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2127793
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT61290.2880449367020194No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46400.21806632506075543No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37000.17388909541482653No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG23590.11086604759015563No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23420.11006709769230373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG1103.8562575E-818.57
GGTATCA30900.016.6440141
TAATACT3000.016.0333334
CGCAAGA4650.013.9247312
GTATTAC1601.7854109E-713.8751
TCTAGCG3200.013.87528
GTGCTAG3900.013.7564111
ACGGACC3900.013.7564118
TGTATCG1356.572791E-613.7037035
CGGGAGT3800.013.6315794
ACGAACG2051.4260877E-913.53658615
ATCTCGC4300.013.33720911
GTAGAAC7500.013.323
TTAATAC2800.013.2142853
TTACACT2408.185452E-1113.1041664
CGGTTTG850.009408168513.05882433
TTAACGG850.009408168513.05882435
GCTTAGG6700.012.9776121
GACGGAC4000.012.9499997
GTATAGA2001.4642865E-812.9499991
Produced by FastQC (version 0.11.5)