FastQCFastQC Report
Fri 10 Feb 2017
ERR1630294.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630294.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467862
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9650.20625740068652723No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8290.17718900017526534No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6380.1363649965160667No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA4810.10280809298468352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG1050.026.428575
GCGCAAG1100.025.2272761
CGCAAGA1100.025.2272762
GACGGAC1200.023.1249987
AGACGGA1450.021.6896556
CGGACCA1450.021.6896559
CAAGACG1301.8189894E-1221.3461534
ACGGACC1351.8189894E-1220.5555538
CGGGTTG701.2172372E-418.514
GCGAAAG1501.2732926E-1118.518
TGACCGG902.1466694E-618.510
TATACTC609.2256616E-418.55
CAGAGCG1601.8189894E-1218.514
GCTATGC500.00702880218.4999988
TACACGG500.00702880218.4999985
TACCGCT500.00702880218.49999836
GTCTCAC953.5971461E-617.5263161
ACCGGGT852.7175312E-517.41176412
TTAATAC852.7175312E-517.4117643
GAGAGTA650.001578041617.0769237
Produced by FastQC (version 0.11.5)