##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1630294.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 467862 Sequences flagged as poor quality 0 Sequence length 43 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.824407624470464 31.0 31.0 34.0 30.0 34.0 2 32.01853110532593 33.0 31.0 34.0 30.0 34.0 3 32.155381287644644 34.0 31.0 34.0 30.0 34.0 4 35.81096990138118 37.0 35.0 37.0 35.0 37.0 5 35.725463918847865 37.0 35.0 37.0 35.0 37.0 6 35.79370412643044 37.0 35.0 37.0 35.0 37.0 7 35.76595662823653 37.0 35.0 37.0 35.0 37.0 8 35.74661545498459 37.0 35.0 37.0 33.0 37.0 9 37.45742761754534 39.0 37.0 39.0 35.0 39.0 10 37.371915222864864 39.0 37.0 39.0 34.0 39.0 11 37.45903920386781 39.0 37.0 39.0 35.0 39.0 12 37.3255276128431 39.0 37.0 39.0 34.0 39.0 13 37.36202127977908 39.0 37.0 39.0 34.0 39.0 14 38.559553885547444 40.0 38.0 41.0 34.0 41.0 15 38.55206449765102 40.0 38.0 41.0 34.0 41.0 16 38.5273200217158 40.0 38.0 41.0 34.0 41.0 17 38.49591118748691 40.0 38.0 41.0 34.0 41.0 18 38.45955645040632 40.0 38.0 41.0 34.0 41.0 19 38.477194129893 40.0 38.0 41.0 34.0 41.0 20 38.42737815851683 40.0 38.0 41.0 34.0 41.0 21 38.38034078424835 40.0 38.0 41.0 34.0 41.0 22 38.33968135903322 40.0 38.0 41.0 34.0 41.0 23 38.276451175774056 40.0 38.0 41.0 34.0 41.0 24 38.251597693336926 40.0 38.0 41.0 34.0 41.0 25 38.211833831343434 40.0 38.0 41.0 33.0 41.0 26 38.05897038015483 40.0 38.0 41.0 33.0 41.0 27 37.94391936083717 40.0 37.0 41.0 33.0 41.0 28 37.839783953387965 40.0 37.0 41.0 33.0 41.0 29 37.7663926542442 40.0 37.0 41.0 33.0 41.0 30 37.6936874548478 40.0 37.0 41.0 32.0 41.0 31 37.59641945701938 40.0 37.0 41.0 32.0 41.0 32 37.493139002526384 40.0 37.0 41.0 32.0 41.0 33 37.41400455689926 40.0 37.0 41.0 32.0 41.0 34 37.31786295959065 39.0 36.0 41.0 31.0 41.0 35 37.212383138617795 39.0 36.0 41.0 31.0 41.0 36 37.09816356105005 39.0 36.0 41.0 31.0 41.0 37 37.04467342934455 39.0 36.0 41.0 31.0 41.0 38 36.930731711487574 39.0 36.0 41.0 30.0 41.0 39 36.83472263188718 39.0 35.0 41.0 30.0 41.0 40 36.727233671467225 39.0 35.0 41.0 30.0 41.0 41 36.58820335911017 39.0 35.0 40.0 30.0 41.0 42 36.50863929962254 39.0 35.0 40.0 30.0 41.0 43 35.27320021715805 38.0 34.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 2.0 14 0.0 15 0.0 16 5.0 17 8.0 18 28.0 19 58.0 20 96.0 21 178.0 22 341.0 23 538.0 24 901.0 25 1326.0 26 2020.0 27 2884.0 28 4134.0 29 5553.0 30 7567.0 31 10048.0 32 13012.0 33 16658.0 34 22223.0 35 29948.0 36 41614.0 37 62373.0 38 110689.0 39 135658.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.447986799526355 18.91433798855218 12.41049711239639 26.227178099525073 2 19.60022399767453 20.788822345050466 33.48615617425651 26.124797483018497 3 20.389986790976828 22.289905997922467 29.224215687531796 28.095891523568916 4 15.067477162069157 15.481488131115587 33.82578623611236 35.6252484707029 5 15.520388490623304 35.58463820528275 33.45879767965768 15.436175624436267 6 35.68530891587691 34.15729424488417 15.271383442126096 14.886013397112825 7 29.89898730822336 29.651050950921427 20.901676135270655 19.548285605584553 8 27.672262333765087 32.63440929162873 19.75967272400836 19.933655650597824 9 27.035963596103123 13.994297463782054 19.22981562939499 39.739923310719824 10 17.940332833185852 25.82855628369049 31.861318080972595 24.36979280215106 11 36.97137190026119 21.173337437107524 20.4355130358952 21.41977762673609 12 22.266822268104697 25.018702095917174 28.055067519909716 24.659408116068413 13 30.930274311656003 18.73073684120531 24.241763596958076 26.09722525018061 14 23.525313019651094 20.48723768974612 23.883538308304587 32.1039109822982 15 26.51636593696432 26.779477709239046 21.131872218731164 25.57228413506547 16 26.164552795482425 25.441048856286685 22.93283062099508 25.461567727235806 17 24.634828218577272 25.528681534298574 24.334098516229144 25.502391730895006 18 24.806246286298098 23.83929449282053 25.904219620315395 25.450239600565975 19 25.227738093711395 24.564508337928707 25.1841354929445 25.0236180754154 20 25.78196134757685 23.93675912982888 25.169173816210762 25.11210570638351 21 26.625586177120603 24.33260234855577 24.293274512569944 24.74853696175368 22 26.146171306923836 24.03678862570587 24.564935814406812 25.25210425296348 23 25.18691409005219 24.15968811316157 24.925512223689893 25.727885573096344 24 25.22880678490666 24.725025755457803 24.882978314118265 25.16318914551727 25 25.578910020476126 24.330892442643343 24.68120941645186 25.40898812042867 26 25.481231645228718 25.01848835767812 24.556172546605623 24.944107450487536 27 25.812953392239592 24.19922968738645 24.489058739542855 25.4987581808311 28 25.06786189089945 24.594004214918076 25.196532310809598 25.141601583372875 29 24.59934767089441 24.694674925512224 25.53915470801219 25.166822695581175 30 24.40420465863866 25.614390568158985 25.24205855572797 24.739346217474385 31 25.70266446088804 24.8663067314721 24.371716446302543 25.059312361337316 32 24.42066250304577 24.736140143888584 24.72588070841402 26.117316644651627 33 24.412326711722688 24.252877985388853 25.40920185866773 25.92559344422073 34 25.38782803476239 23.979720515878615 25.132197100854526 25.500254348504477 35 25.291432088949307 24.266770970927325 25.76678593260406 24.675011007519313 36 24.40612830279014 24.872291402165597 25.287157324168234 25.434422970876025 37 25.191402593072315 23.958346691973272 25.479521739316297 25.37072897563812 38 24.168023904484656 23.920515023660823 26.45480932411694 25.45665174773758 39 24.57669141755475 23.46717621862857 26.31245965690738 25.643672706909303 40 24.198588472669293 23.589648229606166 27.14817617160616 25.063587126118385 41 23.393864002633254 23.484916492470003 27.791100794678776 25.33011871021797 42 24.063505905587547 23.36030709910187 27.687437748737874 24.888749246572708 43 23.18760660194673 22.96061659207202 27.782337526877583 26.06943927910367 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 7.0 1 18.5 2 30.0 3 81.5 4 133.0 5 133.0 6 179.0 7 225.0 8 215.5 9 206.0 10 297.0 11 388.0 12 388.0 13 684.5 14 981.0 15 1560.5 16 2140.0 17 2060.5 18 1981.0 19 1981.0 20 2319.5 21 2658.0 22 2711.0 23 2764.0 24 3328.0 25 3892.0 26 3892.0 27 4703.0 28 5514.0 29 6674.0 30 7834.0 31 9137.5 32 10441.0 33 10441.0 34 12261.5 35 14082.0 36 16276.5 37 18471.0 38 20660.0 39 22849.0 40 22849.0 41 24256.5 42 25664.0 43 26367.5 44 27071.0 45 28790.5 46 30510.0 47 30510.0 48 32295.5 49 34081.0 50 34654.5 51 35228.0 52 36353.0 53 37478.0 54 37478.0 55 36333.0 56 35188.0 57 34488.0 58 33788.0 59 31785.0 60 29782.0 61 29782.0 62 27269.0 63 24756.0 64 21809.5 65 18863.0 66 16464.0 67 14065.0 68 14065.0 69 11936.0 70 9807.0 71 8420.0 72 7033.0 73 5598.5 74 4164.0 75 4164.0 76 3201.0 77 2238.0 78 1863.0 79 1488.0 80 1164.0 81 840.0 82 840.0 83 640.5 84 441.0 85 377.5 86 314.0 87 274.0 88 234.0 89 234.0 90 217.5 91 201.0 92 112.0 93 23.0 94 14.0 95 5.0 96 5.0 97 3.5 98 2.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 467862.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.7056037952995 #Duplication Level Percentage of deduplicated Percentage of total 1 88.98859313185822 60.25026428886703 2 6.7200117908054455 9.099649116160291 3 1.5909908346372625 3.2315698527571013 4 0.7220063264167111 1.955354970962781 5 0.3721247048380817 1.2597463914104963 6 0.25496283009123916 1.0357447414011423 7 0.17383719015912877 0.8238826345261476 8 0.14479597887895942 0.7842799341705105 9 0.11843013258453336 0.7216545270773884 >10 0.7710316244587138 10.2118686653492 >50 0.0943203110337653 4.586823680879442 >100 0.047948786607561564 5.519736948675752 >500 9.463576304123991E-4 0.5194242477627081 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 965 0.20625740068652723 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 829 0.17718900017526534 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 638 0.1363649965160667 No Hit GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA 481 0.10280809298468352 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 2.1373823905339608E-4 0.0 14 0.0 0.0 0.0 2.1373823905339608E-4 0.0 15 0.0 0.0 0.0 6.412147171601883E-4 0.0 16 0.0 0.0 0.0 0.0019236441514805648 0.0 17 0.0 0.0 0.0 0.004488503020121317 0.0 18 0.0 0.0 0.0 0.00491597949822811 0.0 19 0.0 0.0 0.0 0.006839623649708674 0.0 20 0.0 0.0 0.0 0.007694576605922259 0.0 21 0.0 0.0 0.0 0.010686911952669804 0.0 22 0.0 0.0 0.0 0.013465509060363954 0.0 23 0.0 0.0 0.0 0.01752653560237848 0.0 24 0.0 0.0 0.0 0.02415242101303376 0.0 25 0.0 0.0 0.0 0.027358494598834698 0.0 26 0.0 0.0 0.0 0.03184699761895602 0.0 27 0.0 0.0 0.0 0.045526244918373364 0.0 28 0.0 0.0 0.0 0.0822892220355575 0.0 29 0.0 0.0 0.0 0.13123527877878519 0.0 30 0.0 0.0 0.0 0.2126695478581291 0.0 31 0.0 0.0 0.0 0.4317512428878601 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGACGG 105 0.0 26.42857 5 GCGCAAG 110 0.0 25.227276 1 CGCAAGA 110 0.0 25.227276 2 GACGGAC 120 0.0 23.124998 7 AGACGGA 145 0.0 21.689655 6 CGGACCA 145 0.0 21.689655 9 CAAGACG 130 1.8189894E-12 21.346153 4 ACGGACC 135 1.8189894E-12 20.555553 8 CGGGTTG 70 1.2172372E-4 18.5 14 GCGAAAG 150 1.2732926E-11 18.5 18 TGACCGG 90 2.1466694E-6 18.5 10 TATACTC 60 9.2256616E-4 18.5 5 CAGAGCG 160 1.8189894E-12 18.5 14 GCTATGC 50 0.007028802 18.499998 8 TACACGG 50 0.007028802 18.499998 5 TACCGCT 50 0.007028802 18.499998 36 GTCTCAC 95 3.5971461E-6 17.526316 1 ACCGGGT 85 2.7175312E-5 17.411764 12 TTAATAC 85 2.7175312E-5 17.411764 3 GAGAGTA 65 0.0015780416 17.076923 7 >>END_MODULE