FastQCFastQC Report
Fri 10 Feb 2017
ERR1630293.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630293.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111400
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG36790.3310239337772179No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35590.3202267410473277No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34340.30897966528702536No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG25030.22521144502429366No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24860.22368184272089256No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT20940.18841101313658448No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC20560.18499190210545258No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA19590.176264171315458No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG18170.16348749325175455No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA17810.1602483354327875No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC17790.16006838222062264No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC17790.16006838222062264No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT16350.14711175094475437No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG15800.14216303761022134No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT15390.13847399676084218No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA15370.13829404354867733No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA15170.13649451142702895No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT14820.13334533021414433No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC14580.13118589166816627No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC14460.13010617239517724No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG14090.12677703797012776No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA13780.12398776318157279No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG13300.1196688860896167No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12950.11651970487673205No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC12940.11642972827064964No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG12900.11606982184631995No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC12610.11346050026992982No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC11910.10716213784416051No Hit
TAGCAGATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGC11600.10437286305560553No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG11270.10140363505488574No Hit
GCAGAGATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAA11200.1007737988123088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11100.024.8333341
CTTAGCG450.003825730620.55555516
GTAACTC555.1429466E-420.1818183
CGAACGA1205.173206E-918.516
CCGACCA1653.6379788E-1217.9393949
GACGGAC2850.017.5263167
ACGGACC2550.017.4117648
CGTCAGA1301.3928002E-817.0769231
TCTATAC1005.8774385E-616.653
AAGACGG2900.016.5862065
GACCATA1901.8189894E-1216.55263111
CGCAAGA2800.016.5178572
CGATGCG1801.0913936E-1116.44444533
GGTGCTT803.382439E-416.18758
CGTAATT803.382439E-416.187535
TCCGACC1851.8189894E-1116.08
TTCCGAC1851.8189894E-1116.07
GTATCAA17150.015.9650142
CGGACCA2900.015.9482769
GCGCAAG3250.015.9384621
Produced by FastQC (version 0.11.5)