FastQCFastQC Report
Fri 10 Feb 2017
ERR1630292.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630292.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2373931
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT90030.37924438410383454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT78690.33147551466323155No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59750.2516922353682563No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA33290.1402315400068494No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32210.13568212386964912No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC27190.11453576367636634No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC26780.11280867051316992No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA25060.10556330407244356No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG24070.10139300594667663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36600.021.9371571
TCTAGCG2650.019.5471728
TACGGCT609.2407665E-418.54
CTAGCGG2850.018.17543829
ATGATCG2000.017.57515
ACGGACC3800.017.5263168
AAGACGG4000.017.1125015
TGATCGG2100.016.73809416
GACGGAC3900.016.6025627
CTTATAC12400.016.11290437
CGGACCA4150.016.0481939
TCTATCG2200.015.97727331
GTACTAT4100.015.7926821
CGCAAGA4350.015.3103462
CGGTCCA3800.015.09210610
GTATCAA53500.014.9728982
TATCGCC2350.014.95744733
AGACGGA4350.014.8850586
GTAAACG3650.014.69863127
CCGGTCC4300.014.6279079
Produced by FastQC (version 0.11.5)