FastQCFastQC Report
Fri 10 Feb 2017
ERR1630291.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630291.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1933021
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG100820.5215670186718095No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG70990.3672489848791089No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT63490.3284496133254631No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA56920.29446136384446936No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC53180.27511341056305133No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT48940.25317883251139023No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC40540.2097235363713069No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC40280.2083784914907805No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT40210.2080163640229465No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA39890.20636092416999094No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC39160.20258445200543604No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA37860.1958592276028041No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC37270.19280701037391731No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC35690.18463327609994926No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC35500.1836503586872569No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA35300.18261570877915967No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC34240.1771320642662444No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT33540.1735107895879041No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG32870.17004471239577842No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA32030.1656991827817701No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA31900.1650266603415069No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC30820.15943955083778188No Hit
GCCTTGTACCAGCATTACAAATAATCCAGCCACAAAGTAAATG30320.15685292606753884No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA29750.15390417382946175No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG28730.14862745929816593No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT28320.1465064269865666No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC27990.14479925463820623No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA27820.14391980221632356No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG27020.13978120258393467No Hit
GCCTGGGAAGCTGAGAATGATCTGGATTTCTCCTCTGTGTCTT26750.13838442520800343No Hit
GTGTTCATCTCATCAGAGAAAGAACCATCAGCATGTCTGCGGC26510.13714284531828677No Hit
TTCCCAGGCAGACCCACTCAGTGATCCTGATCAGATGAACGAG26100.13502181300668747No Hit
GTCCAGATACTTGCTGTAGTCACTGGTGAATGTGCCCTGTGAA26000.13450448805263884No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG25190.1303141559248451No Hit
GATCAGGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGA24960.1291243085305333No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT24330.12586516132002704No Hit
CTTCAGCATGTCTCTCAAATTCATCGTGACGTTTGGCAATGTT24160.12498570889814441No Hit
ACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTC23380.12095057425656525No Hit
CTTCTGGTAGTGTGCTGTAGAACAGAGCAGGTGAAGAGAGAGC22940.11867434445875136No Hit
CTGGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGAC22380.11577732471607914No Hit
CTACAGCAAGTATCTGGACTCCAGGCGTGCCCAAGATTTTGTG21610.11179392256990484No Hit
GTTGAAGAACTTGGCCGCAGACATGCTGATGGTTCTTTCTCTG21550.11148352759747565No Hit
ATGTTATTCCTGTTCCTCTTGGTATTCATCAACCACTGCACAA21160.10946596027668608No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG20740.10729319546968191No Hit
TGTTATTCCTGTTCCTCTTGGTATTCATCAACCACTGCACAAA20740.10729319546968191No Hit
TGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGA20580.10646547554320412No Hit
TTGTAATGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGA20350.10527562814889233No Hit
CTGGTGAATGTGCCCTGTGAATGGCGCTTGTCCTCGTTCATCT20280.1049135006810583No Hit
GCCTTTCACCAGCCAAGCAATGAATTCCTTGGCAGCTTGGCCT19820.10253380589243469No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC405.942195E-527.7525
GCTTAGG24800.019.2459681
CTTAGGA26200.018.641222
CTTATAC6600.018.537
GTGCTAT902.1537617E-618.51
CGCATAG500.00703657618.49999827
TAGGACA26650.018.4652924
GGTATCA10050.018.407961
TCGCCAT13650.018.0256413
TTAGGAC27600.017.896743
TCTTATA12350.017.52631637
TCTAGCG852.7246708E-517.41176428
AGGTCGC14450.017.1557110
CGTACTA650.001580460617.07692327
TGTTGAA14700.016.86394520
GAGGTCG14750.016.6813569
GTGTAAG2450.016.6122441
TACCGCA1901.8189894E-1216.55263128
GTCGCCA15000.016.2812
GGACACA30350.016.2141696
Produced by FastQC (version 0.11.5)