FastQCFastQC Report
Fri 10 Feb 2017
ERR1630290.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630290.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1796167
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42890.2387862598522298No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41010.22831952708183595No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30860.17181030494380534No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG24290.1352324143579077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18250.019.0575351
CTAATCG609.2395727E-418.526
CGTATAC500.00703638718.4999983
ACCGACG500.00703638718.49999815
AAGACGG4050.017.8148165
TCGCCAT3950.017.32911313
TGTGCGA650.001580402117.07692310
CGCAAGA4100.016.6951242
CAAGACG4450.016.6292154
GTAAACG3450.016.62318827
GACGGAC4250.016.5411787
TAACGCT904.4481996E-516.44444532
TACGTTA904.4481996E-516.44444519
AGCGTTC803.3838904E-416.187537
ACGCTTC3800.016.0657931
GCTTCGG3600.015.93055533
GTACCGT700.002593254715.8571436
TACGCTA2450.015.8571439
CGTCAGA1403.4762706E-815.8571431
GAGGTCG4550.015.8571429
Produced by FastQC (version 0.11.5)