FastQCFastQC Report
Fri 10 Feb 2017
ERR1630289.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630289.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2157491
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT46130.21381317465518976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39160.18150713027308107No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG37170.1722834533261089No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35460.16435758017067048No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA30590.14178506422506512No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA30550.14159966368341745No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC29920.13867960515246647No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC27190.12602601818501213No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA27120.1257015672371287No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC26150.12120560410217238No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG25550.11842459597745715No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT24850.11518008649862271No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC24670.11434578406120813No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA23580.10929361930130878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16400.021.996951
TATACGA759.271949E-619.7333345
TTATACG752.068325E-417.2666664
AATACGC752.068325E-417.2666665
CGGCCTT7600.015.82236924
TTATTCG957.0642636E-515.57894814
ATTAGAC1201.9373183E-615.4166663
GTCTATA1201.9373183E-615.4166661
ATACCGC2055.456968E-1215.34146327
ACGGACC1701.4879333E-915.2352948
TCGCCAT7350.015.10204113
CCTTATA1601.097942E-815.031251
ATCTCGC8150.014.98159511
AAGACCG750.004105956814.85
TATTCGA1001.09407E-414.815
ACCGTTC1001.09407E-414.88
AAGACGG2500.014.7999995
AATGCGT1252.961473E-614.79999935
TTATACT3800.014.6052644
TCTCGCC8500.014.58235412
Produced by FastQC (version 0.11.5)