FastQCFastQC Report
Fri 10 Feb 2017
ERR1630288.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630288.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2091556
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38040.1818741644976276No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34880.16676579541738304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27770.13277196498683277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12300.021.8089431
TATTACG650.001580519417.0769237
ATTACGG700.002593446315.8571438
ACCGTAT700.002593446315.8571438
ACGAACG1902.7284841E-1115.57894815
CTTATAC8900.014.34269737
CTAGTAC1956.730261E-1014.230773
TCGATTG800.006300898313.87513
CGTATTG3350.013.8059727
TCTTATA15150.013.7986837
GTATCAA20100.013.3457722
ATTCGTA3800.013.14473724
CGCTAGA3250.013.09230737
CAATACG1703.735131E-713.0588244
GTATAGA3150.012.9206351
CCGATAA2451.1459633E-1012.8367349
TACGACG1602.697805E-612.718755
CGATAAC2358.094503E-1012.59574510
TCCGCTA1205.124561E-412.33333428
TTGCGCG1951.4689431E-712.33333418
Produced by FastQC (version 0.11.5)