##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630288.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2091556
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.881339538601882	33.0	31.0	34.0	30.0	34.0
2	32.090377690102486	33.0	31.0	34.0	30.0	34.0
3	32.21990948365714	34.0	31.0	34.0	30.0	34.0
4	35.847891713155185	37.0	35.0	37.0	35.0	37.0
5	35.77605811175986	37.0	35.0	37.0	35.0	37.0
6	35.829967736938436	37.0	35.0	37.0	35.0	37.0
7	35.810109793856824	37.0	35.0	37.0	35.0	37.0
8	35.80907515744259	37.0	35.0	37.0	35.0	37.0
9	37.56605608456097	39.0	37.0	39.0	35.0	39.0
10	37.45178900301976	39.0	37.0	39.0	34.0	39.0
11	37.52471461438279	39.0	37.0	39.0	35.0	39.0
12	37.44184186318702	39.0	37.0	39.0	34.0	39.0
13	37.45265104066064	39.0	37.0	39.0	35.0	39.0
14	38.713214468080224	40.0	38.0	41.0	35.0	41.0
15	38.719704851316436	40.0	38.0	41.0	35.0	41.0
16	38.68045273471043	40.0	38.0	41.0	34.0	41.0
17	38.64710722543408	40.0	38.0	41.0	34.0	41.0
18	38.60661392762135	40.0	38.0	41.0	34.0	41.0
19	38.642430802713385	40.0	38.0	41.0	34.0	41.0
20	38.62062502749149	40.0	38.0	41.0	34.0	41.0
21	38.56752484752978	40.0	38.0	41.0	34.0	41.0
22	38.53616350697758	40.0	38.0	41.0	34.0	41.0
23	38.47703193220741	40.0	38.0	41.0	34.0	41.0
24	38.45039626000929	40.0	38.0	41.0	34.0	41.0
25	38.43622547041533	40.0	38.0	41.0	34.0	41.0
26	38.282626905519145	40.0	38.0	41.0	34.0	41.0
27	38.17250649755493	40.0	38.0	41.0	33.0	41.0
28	38.11235893277541	40.0	38.0	41.0	33.0	41.0
29	38.061062672957355	40.0	38.0	41.0	33.0	41.0
30	38.006184869063986	40.0	37.0	41.0	33.0	41.0
31	37.960900879536574	40.0	37.0	41.0	33.0	41.0
32	37.887471337128915	40.0	37.0	41.0	33.0	41.0
33	37.80093002530174	40.0	37.0	41.0	33.0	41.0
34	37.74914178726269	40.0	37.0	41.0	33.0	41.0
35	37.644941373790616	40.0	37.0	41.0	32.0	41.0
36	37.57229498038781	40.0	37.0	41.0	32.0	41.0
37	37.511354226231575	40.0	37.0	41.0	32.0	41.0
38	37.42473928501078	40.0	37.0	41.0	32.0	41.0
39	37.35712168356955	40.0	37.0	41.0	31.0	41.0
40	37.26614730851099	40.0	36.0	41.0	31.0	41.0
41	37.158256341211995	40.0	36.0	41.0	31.0	41.0
42	37.12723063594759	39.0	36.0	41.0	31.0	41.0
43	35.954367944248204	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	4.0
12	0.0
13	1.0
14	6.0
15	3.0
16	6.0
17	28.0
18	57.0
19	124.0
20	268.0
21	506.0
22	992.0
23	1756.0
24	2881.0
25	4723.0
26	7303.0
27	11051.0
28	15718.0
29	22540.0
30	30469.0
31	40189.0
32	52552.0
33	67762.0
34	90095.0
35	120383.0
36	167579.0
37	255457.0
38	485076.0
39	714023.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.87585701745495	19.753810082063307	12.356111909028494	26.01422099145325
2	18.221314657604196	20.94942712506861	35.2996046962166	25.5296535211106
3	19.166161460654173	23.604197066681458	29.924037415206666	27.3056040574577
4	14.072059270705637	16.00172311905586	35.66775166431116	34.25846594592734
5	14.396697960752663	36.55522491389186	34.707079322762574	14.340997802592902
6	33.94420230679934	36.06855374658867	16.044514227685035	13.94272971892696
7	28.68228247295315	30.66740742299035	22.275234323154628	18.375075780901874
8	26.513418717930577	34.37560361759379	20.14968760100136	18.961290063474276
9	26.38385010967911	14.395263621915932	20.268068366326315	38.95281790207864
10	16.341565800772248	26.741908894621996	34.02170441527743	22.894820889328326
11	35.46914354671833	22.160009103270482	22.109472564922957	20.261374785088233
12	20.9509092751999	25.75996052699521	30.448957618156054	22.840172579648836
13	29.639225533526236	20.400123161894783	25.000095622589114	24.960555681989867
14	22.21226684822209	20.96319677790124	25.649086134915823	31.17545023896085
15	24.777390612539183	28.366058570748287	22.357756617561282	24.498794199151252
16	24.533457387705614	26.757304131469585	24.448496717276516	24.260741763548285
17	23.046812994727368	26.771025973007657	26.025169777906974	24.156991254357997
18	23.4410171183559	25.83578924016378	26.845755026401395	23.877438615078916
19	23.729271413244494	25.905306862450733	26.835953711017062	23.529468013287715
20	24.067106020589456	25.750589513261897	26.50132246040747	23.68098200574118
21	24.358420238329742	25.721137755814333	26.2134984671699	23.706943538686033
22	24.22478767004087	25.678968194014406	26.387149089003593	23.70909504694113
23	23.885327478680942	25.754175360353727	26.496780387424483	23.863716773540848
24	23.8047176360566	26.311559432307813	25.997056736707023	23.88666619492856
25	24.225600462048348	25.697614598891928	26.418656732117142	23.658128206942582
26	23.904691052976826	26.20092409670121	26.291335254709892	23.60304959561207
27	23.96373800175563	25.737728275025866	26.24323709238481	24.055296630833695
28	23.890777966260526	25.68489679453957	26.50443019455372	23.919895044646186
29	23.5133077957272	26.008005523160747	26.819936927340223	23.658749753771833
30	23.559206638502626	26.29893725054457	26.446578528138858	23.695277582813944
31	24.000122396914065	26.019002120909025	26.343067075421363	23.637808406755546
32	23.39449672875123	26.037983204848448	26.425684992417132	24.1418350739832
33	23.258138916672564	25.804711898701253	26.843460084262627	24.09368910036356
34	23.97114875241208	25.45004771567197	26.59355044760934	23.985253084306613
35	23.87930325556667	25.654058509549827	27.021891835552097	23.444746399331407
36	23.79505975455594	25.81001895239716	26.36921985354444	24.025701439502456
37	23.680408270206488	25.3210528429552	26.841786688953107	24.15675219788521
38	23.330238348865628	25.518417866889532	27.076587956526144	24.074755827718693
39	23.253118730744003	25.243789790949894	27.396206460644613	24.10688501766149
40	23.30551990957928	25.154717349188832	27.768991124311277	23.77077161692061
41	22.76152300010136	25.194591968849984	27.950387175863327	24.093497855185326
42	22.873736108428368	25.321674389784448	27.857394207948534	23.947195293838654
43	22.805461579799918	24.657623319672055	28.13393473567048	24.40298036485755
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	217.0
1	226.0
2	235.0
3	518.0
4	801.0
5	801.0
6	1055.5
7	1310.0
8	1220.0
9	1130.0
10	1641.0
11	2152.0
12	2152.0
13	3669.0
14	5186.0
15	7451.0
16	9716.0
17	10442.5
18	11169.0
19	11169.0
20	14503.0
21	17837.0
22	18923.5
23	20010.0
24	24234.0
25	28458.0
26	28458.0
27	33763.0
28	39068.0
29	46019.0
30	52970.0
31	60111.5
32	67253.0
33	67253.0
34	75830.5
35	84408.0
36	93413.0
37	102418.0
38	111415.0
39	120412.0
40	120412.0
41	128081.0
42	135750.0
43	139869.0
44	143988.0
45	147150.5
46	150313.0
47	150313.0
48	151403.0
49	152493.0
50	150935.5
51	149378.0
52	148525.5
53	147673.0
54	147673.0
55	141333.0
56	134993.0
57	127196.0
58	119399.0
59	113431.0
60	107463.0
61	107463.0
62	97386.0
63	87309.0
64	76737.5
65	66166.0
66	56551.5
67	46937.0
68	46937.0
69	39808.5
70	32680.0
71	27404.0
72	22128.0
73	17572.0
74	13016.0
75	13016.0
76	10126.5
77	7237.0
78	5685.5
79	4134.0
80	3333.5
81	2533.0
82	2533.0
83	1886.5
84	1240.0
85	1056.0
86	872.0
87	731.5
88	591.0
89	591.0
90	506.5
91	422.0
92	244.5
93	67.0
94	41.0
95	15.0
96	15.0
97	11.0
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2091556.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.01176087188914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.5162093718412	47.288988452621005
2	11.067162869470591	12.840512118279571
3	3.063651513202065	5.331834569360385
4	1.2966789015906923	3.0089050546681246
5	0.702748591772557	2.0383841629483213
6	0.44434830680173765	1.546645663080674
7	0.30060432036122625	1.220701016489655
8	0.2192338800582725	1.0174514739965546
9	0.1659014968936475	0.8661814169474467
>10	1.0335980837673888	11.592545516215287
>50	0.11588106080201437	4.752276383547298
>100	0.07191599526533561	7.27575710748922
>500	0.001735110865580081	0.6570015521684481
>1k	3.3049730772953745E-4	0.5628155121880707
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3804	0.1818741644976276	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3488	0.16676579541738304	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2777	0.13277196498683277	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	4.7811294557735964E-5
17	0.0	0.0	0.0	1.9124517823094385E-4	4.7811294557735964E-5
18	0.0	0.0	0.0	2.868677673464158E-4	4.7811294557735964E-5
19	0.0	0.0	0.0	4.303016510196237E-4	4.7811294557735964E-5
20	0.0	0.0	0.0	4.303016510196237E-4	4.7811294557735964E-5
21	0.0	0.0	0.0	5.737355346928316E-4	9.562258911547193E-5
22	0.0	0.0	0.0	7.649807129237754E-4	9.562258911547193E-5
23	0.0	0.0	0.0	0.001338716247616607	9.562258911547193E-5
24	0.0	0.0	0.0	0.00248618731700227	9.562258911547193E-5
25	0.0	0.0	0.0	0.0031077341462528375	9.562258911547193E-5
26	0.0	0.0	0.0	0.004781129455773596	9.562258911547193E-5
27	0.0	0.0	0.0	0.01467806742922494	9.562258911547193E-5
28	0.0	0.0	0.0	0.03743624363870726	9.562258911547193E-5
29	0.0	0.0	0.0	0.06865701898490885	9.562258911547193E-5
30	0.0	0.0	0.0	0.11536865376781688	9.562258911547193E-5
31	0.0	0.0	0.0	0.27242875638997954	9.562258911547193E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1230	0.0	21.808943	1
TATTACG	65	0.0015805194	17.076923	7
ATTACGG	70	0.0025934463	15.857143	8
ACCGTAT	70	0.0025934463	15.857143	8
ACGAACG	190	2.7284841E-11	15.578948	15
CTTATAC	890	0.0	14.342697	37
CTAGTAC	195	6.730261E-10	14.23077	3
TCGATTG	80	0.0063008983	13.875	13
CGTATTG	335	0.0	13.80597	27
TCTTATA	1515	0.0	13.79868	37
GTATCAA	2010	0.0	13.345772	2
ATTCGTA	380	0.0	13.144737	24
CGCTAGA	325	0.0	13.092307	37
CAATACG	170	3.735131E-7	13.058824	4
GTATAGA	315	0.0	12.920635	1
CCGATAA	245	1.1459633E-10	12.836734	9
TACGACG	160	2.697805E-6	12.71875	5
CGATAAC	235	8.094503E-10	12.595745	10
TCCGCTA	120	5.124561E-4	12.333334	28
TTGCGCG	195	1.4689431E-7	12.333334	18
>>END_MODULE