FastQCFastQC Report
Fri 10 Feb 2017
ERR1630287.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630287.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1844845
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC98790.5354921416162334No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT61160.3315183660415916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57130.3096737124257051No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47370.2567695389043524No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG47300.256390103233605No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG40970.2220782775788752No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT40480.21942222788364335No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39580.2145437692597481No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG38540.20890643929435807No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG38140.20673823546151576No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT35030.18988045066116666No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT32380.17551610026858624No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG32130.1741609728730598No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC29820.16163959573839537No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG29620.1605554938219742No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT29610.16050128872615316No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG28820.15621908615628954No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC28010.15182847339478384No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG27400.1485219625496993No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT27170.14727524534581496No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG27090.1468416045792465No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT26040.14115006951803538No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT23470.12721935989202343No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA21910.11876336494393838No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA21590.11702880187766451No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT21460.11632413563199076No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21250.11518582861974853No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC21070.1142101368949695No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA20440.11079521585824284No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG20180.10938588336689532No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA20150.10922326807943215No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC20080.10884383240868474No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT19500.10569993685106338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCATCA22850.020.64551514
CAGGACA24800.020.3649184
TTAGTCG555.1447167E-420.1818185
GCATCAG23500.019.99574515
GACAGGC24400.019.5614767
ACAGGCT25000.018.9448
CCAGGAC26200.018.9236643
GCTGCAT25000.018.79612
GGACAGG25750.018.5359236
TCCAGGA26400.018.52
TATACTG1501.2732926E-1118.55
CTGCATC25350.018.31755313
CTAACCG752.0681208E-417.26666621
CATCAGA27400.016.9470816
ATCAGAA27300.016.87362717
GGCCATC27700.016.4963926
ATCAAGC27300.016.46703330
AGGCCAT27800.016.37050425
AGAGGCC28800.016.12326423
AAGAGGC28600.016.10664422
Produced by FastQC (version 0.11.5)