##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630287.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1844845
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.791880076646006	31.0	31.0	34.0	30.0	34.0
2	32.00281649677886	33.0	31.0	34.0	30.0	34.0
3	32.11409793234662	34.0	31.0	34.0	30.0	34.0
4	35.7755404925617	37.0	35.0	37.0	35.0	37.0
5	35.71304201708003	37.0	35.0	37.0	33.0	37.0
6	35.76057121329976	37.0	35.0	37.0	35.0	37.0
7	35.73701693096168	37.0	35.0	37.0	33.0	37.0
8	35.7359854079882	37.0	35.0	37.0	33.0	37.0
9	37.48726207350753	39.0	37.0	39.0	35.0	39.0
10	37.375161598941915	39.0	37.0	39.0	34.0	39.0
11	37.44828264705165	39.0	37.0	39.0	35.0	39.0
12	37.37153853033724	39.0	37.0	39.0	34.0	39.0
13	37.36367770734127	39.0	37.0	39.0	34.0	39.0
14	38.59382495548407	40.0	38.0	41.0	34.0	41.0
15	38.60168252617429	40.0	38.0	41.0	34.0	41.0
16	38.56817727234537	40.0	38.0	41.0	34.0	41.0
17	38.53491756760053	40.0	38.0	41.0	34.0	41.0
18	38.416692459258094	40.0	38.0	41.0	34.0	41.0
19	38.452520943493894	40.0	38.0	41.0	34.0	41.0
20	38.432283470969104	40.0	38.0	41.0	34.0	41.0
21	38.38687586219981	40.0	38.0	41.0	34.0	41.0
22	38.339607934541924	40.0	38.0	41.0	34.0	41.0
23	38.28312785084926	40.0	38.0	41.0	34.0	41.0
24	38.2315701319081	40.0	38.0	41.0	33.0	41.0
25	38.213229837737046	40.0	38.0	41.0	33.0	41.0
26	38.01002198016636	40.0	37.0	41.0	33.0	41.0
27	37.8892768769192	40.0	37.0	41.0	33.0	41.0
28	37.79890505706442	40.0	37.0	41.0	33.0	41.0
29	37.72464570194244	40.0	37.0	41.0	32.0	41.0
30	37.66527757074443	40.0	37.0	41.0	32.0	41.0
31	37.626183229485406	40.0	37.0	41.0	32.0	41.0
32	37.531762288972786	40.0	37.0	41.0	32.0	41.0
33	37.435074491352935	40.0	37.0	41.0	32.0	41.0
34	37.33774382129664	40.0	36.0	41.0	31.0	41.0
35	37.17432413021148	39.0	36.0	41.0	31.0	41.0
36	37.09332979193374	39.0	36.0	41.0	31.0	41.0
37	36.99347316441219	39.0	36.0	41.0	31.0	41.0
38	36.88771143375189	39.0	36.0	41.0	30.0	41.0
39	36.81083722480751	39.0	35.0	41.0	30.0	41.0
40	36.67545457748483	39.0	35.0	41.0	30.0	41.0
41	36.5274985161355	39.0	35.0	41.0	30.0	41.0
42	36.50229477273158	39.0	35.0	41.0	30.0	41.0
43	35.21470692659817	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	5.0
11	4.0
12	1.0
13	6.0
14	6.0
15	6.0
16	15.0
17	41.0
18	106.0
19	196.0
20	390.0
21	784.0
22	1342.0
23	2299.0
24	3784.0
25	5844.0
26	8626.0
27	12431.0
28	17202.0
29	23375.0
30	30991.0
31	40551.0
32	52006.0
33	66457.0
34	88887.0
35	115224.0
36	155275.0
37	233772.0
38	423500.0
39	561712.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.3462973854172	17.61849911510181	11.517769785537538	27.517433713943447
2	19.083879675528294	19.88687396502145	33.951199152232306	27.07804720721795
3	20.16174800593004	21.957617035577513	29.41108873645211	28.469546222040332
4	15.139049622054971	14.660743856529953	33.201380061739606	36.99882645967548
5	15.460973686136233	36.116475909900295	32.80449035013782	15.61806005382566
6	36.28440329675392	33.46806913317921	15.326924484170757	14.920603085896106
7	30.71342036864886	29.746455664297	20.370546035032753	19.16957793202139
8	28.904596321100147	32.5156313945074	19.14551086947684	19.434261414915614
9	27.963975293317322	13.361068274028442	18.235461515737093	40.43949491691714
10	17.949095994514444	26.072163244066576	31.493269082226423	24.48547167919256
11	36.87751545522794	21.035263125086388	20.62899593190756	21.45822548777811
12	23.644967463391232	23.901032336049912	28.19591889833563	24.25808130222322
13	30.595632695429696	18.326092435949903	24.419720897961618	26.658553970658783
14	23.33139098406641	19.256685521005828	24.733514197669724	32.678409297258035
15	26.253208264108906	26.047987771330384	21.426949147489356	26.271854817071354
16	25.46940257853641	25.11294986841713	22.194601714507183	27.223045838539285
17	24.37689887226298	25.75679799657966	24.85010935878082	25.016193772376543
18	24.553715894831274	23.583715705113438	25.685518295574965	26.17705010448032
19	25.577650154891064	23.778366204206858	24.98193615181763	25.66204748908445
20	25.243855174824986	24.58130628860419	24.937867408915114	25.23697112765571
21	27.58930967100217	23.67141955015191	23.92650873108581	24.81276204776011
22	26.724955213039575	24.315972344560112	24.283503492163298	24.67556895023701
23	25.918437592318057	24.33429366694763	24.007165913667546	25.740102827066774
24	26.160842780829825	23.953502868804698	24.53300954822763	25.352644802137846
25	25.758695174933393	23.71603034401264	25.006111624553824	25.519162856500138
26	26.34226723654291	24.25293181812022	25.00800880290756	24.39679214242931
27	26.871742612523004	23.928839550206117	23.822109716534452	25.37730812073643
28	25.208838682924583	23.35789727592291	25.56182226691131	25.871441774241195
29	25.217348882968487	23.595695031289893	25.854638194536665	25.33231789120495
30	24.84203279950348	24.207941588588746	25.448425206453656	25.501600405454116
31	24.854662586829786	24.409476134851438	24.946648634438123	25.78921264388065
32	24.561575633725326	24.109667749865167	25.515639525271773	25.813117091137737
33	25.740048621970953	24.296024869297963	24.839593570191536	25.124332938539553
34	26.026739373768525	24.580547417262697	24.6636980342522	24.729015174716576
35	26.14973073618651	24.111293902739796	25.44538972108768	24.293585639986016
36	25.747257899715155	24.769831611869833	23.73191243708821	25.750998051326807
37	24.67649043686597	24.135144144901062	25.231550618073605	25.95681480015936
38	25.81924226696552	23.93631985342942	25.421810504405517	24.822627375199545
39	24.175147505617005	24.366979339727727	25.655217647010996	25.802655507644275
40	25.126880578043142	23.08394472164328	26.708639479197437	25.080535221116136
41	23.67136534505609	23.43362179478493	27.136155069938123	25.75885779022086
42	24.030961950732987	24.077307307659993	25.894424734869325	25.99730600673769
43	24.004726684355596	21.987809273949843	27.517813149614195	26.489650892080363
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	342.0
1	300.0
2	258.0
3	564.0
4	870.0
5	870.0
6	1185.0
7	1500.0
8	1404.0
9	1308.0
10	1777.5
11	2247.0
12	2247.0
13	4029.0
14	5811.0
15	9096.5
16	12382.0
17	11460.5
18	10539.0
19	10539.0
20	13312.5
21	16086.0
22	15981.0
23	15876.0
24	18831.5
25	21787.0
26	21787.0
27	24769.0
28	27751.0
29	31703.0
30	35655.0
31	41030.5
32	46406.0
33	46406.0
34	52958.0
35	59510.0
36	66462.5
37	73415.0
38	77172.5
39	80930.0
40	80930.0
41	85979.0
42	91028.0
43	93572.0
44	96116.0
45	97664.5
46	99213.0
47	99213.0
48	100336.5
49	101460.0
50	108553.0
51	115646.0
52	125119.0
53	134592.0
54	134592.0
55	138648.0
56	142704.0
57	136123.0
58	129542.0
59	116245.0
60	102948.0
61	102948.0
62	101472.0
63	99996.0
64	87741.0
65	75486.0
66	63539.5
67	51593.0
68	51593.0
69	45136.5
70	38680.0
71	36125.5
72	33571.0
73	40040.0
74	46509.0
75	46509.0
76	44836.5
77	43164.0
78	32360.5
79	21557.0
80	13208.5
81	4860.0
82	4860.0
83	3255.5
84	1651.0
85	1241.0
86	831.0
87	681.5
88	532.0
89	532.0
90	456.5
91	381.0
92	231.0
93	81.0
94	50.0
95	19.0
96	19.0
97	12.0
98	5.0
99	6.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1844845.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.05744152984331
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.74159957319978	40.10033741618113
2	11.156820424204748	10.94650131238772
3	3.1101378406151623	4.577262157971944
4	1.2127846041862984	2.37984439232654
5	0.6260612910101876	1.5356482588915248
6	0.3897879444794223	1.147319957720224
7	0.26093599080779595	0.8960596478459627
8	0.18919327099655156	0.7425070263802503
9	0.13319521069281892	0.5880794634556313
>10	0.9425100047553356	9.128557853048944
>50	0.12247847036713264	4.232549406428788
>100	0.09079559664729979	8.93723782885404
>500	0.013574520753928572	4.600984564211184
>1k	0.009791457592997658	9.002189750967919
>5k	3.337996906703747E-4	1.1849209633281887
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	9879	0.5354921416162334	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	6116	0.3315183660415916	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5713	0.3096737124257051	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4737	0.2567695389043524	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	4730	0.256390103233605	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	4097	0.2220782775788752	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	4048	0.21942222788364335	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3958	0.2145437692597481	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	3854	0.20890643929435807	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	3814	0.20673823546151576	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	3503	0.18988045066116666	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	3238	0.17551610026858624	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	3213	0.1741609728730598	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	2982	0.16163959573839537	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	2962	0.1605554938219742	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	2961	0.16050128872615316	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	2882	0.15621908615628954	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	2801	0.15182847339478384	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	2740	0.1485219625496993	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	2717	0.14727524534581496	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	2709	0.1468416045792465	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	2604	0.14115006951803538	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	2347	0.12721935989202343	No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA	2191	0.11876336494393838	No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA	2159	0.11702880187766451	No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT	2146	0.11632413563199076	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2125	0.11518582861974853	No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC	2107	0.1142101368949695	No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA	2044	0.11079521585824284	No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG	2018	0.10938588336689532	No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA	2015	0.10922326807943215	No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC	2008	0.10884383240868474	No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT	1950	0.10569993685106338	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.420509582105814E-5	0.0
5	1.0841019164211628E-4	0.0	0.0	5.420509582105814E-5	0.0
6	1.0841019164211628E-4	0.0	0.0	5.420509582105814E-5	5.420509582105814E-5
7	1.0841019164211628E-4	0.0	0.0	5.420509582105814E-5	5.420509582105814E-5
8	1.0841019164211628E-4	0.0	0.0	5.420509582105814E-5	5.420509582105814E-5
9	1.0841019164211628E-4	0.0	0.0	1.0841019164211628E-4	5.420509582105814E-5
10	1.0841019164211628E-4	0.0	0.0	1.0841019164211628E-4	5.420509582105814E-5
11	1.0841019164211628E-4	0.0	0.0	1.0841019164211628E-4	5.420509582105814E-5
12	1.0841019164211628E-4	0.0	0.0	1.0841019164211628E-4	5.420509582105814E-5
13	1.626152874631744E-4	0.0	0.0	1.626152874631744E-4	5.420509582105814E-5
14	1.626152874631744E-4	0.0	0.0	2.1682038328423256E-4	5.420509582105814E-5
15	2.1682038328423256E-4	0.0	0.0	2.1682038328423256E-4	5.420509582105814E-5
16	2.1682038328423256E-4	0.0	0.0	4.878458623895232E-4	1.0841019164211628E-4
17	2.710254791052907E-4	0.0	0.0	9.214866289579883E-4	1.0841019164211628E-4
18	2.710254791052907E-4	0.0	0.0	9.214866289579883E-4	1.0841019164211628E-4
19	2.710254791052907E-4	0.0	0.0	0.0010841019164211628	1.0841019164211628E-4
20	2.710254791052907E-4	0.0	0.0	0.0013009222997053952	1.0841019164211628E-4
21	2.710254791052907E-4	0.0	0.0	0.0017887681620949185	2.1682038328423256E-4
22	2.710254791052907E-4	0.0	0.0	0.002710254791052907	2.1682038328423256E-4
23	3.252305749263488E-4	0.0	0.0	0.003956971994937244	2.1682038328423256E-4
24	3.252305749263488E-4	0.0	0.0	0.006016765636137453	2.1682038328423256E-4
25	3.252305749263488E-4	0.0	0.0	0.007263482840021791	2.1682038328423256E-4
26	3.252305749263488E-4	0.0	0.0	0.010082147822716814	2.1682038328423256E-4
27	3.252305749263488E-4	0.0	0.0	0.02184465361588643	2.1682038328423256E-4
28	3.7943567074740696E-4	0.0	0.0	0.05474714677926872	2.1682038328423256E-4
29	3.7943567074740696E-4	0.0	0.0	0.09935794063999957	2.1682038328423256E-4
30	3.7943567074740696E-4	0.0	0.0	0.16472928620019567	2.1682038328423256E-4
31	3.7943567074740696E-4	0.0	0.0	0.3161783239242321	2.1682038328423256E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCATCA	2285	0.0	20.645515	14
CAGGACA	2480	0.0	20.364918	4
TTAGTCG	55	5.1447167E-4	20.181818	5
GCATCAG	2350	0.0	19.995745	15
GACAGGC	2440	0.0	19.561476	7
ACAGGCT	2500	0.0	18.944	8
CCAGGAC	2620	0.0	18.923664	3
GCTGCAT	2500	0.0	18.796	12
GGACAGG	2575	0.0	18.535923	6
TCCAGGA	2640	0.0	18.5	2
TATACTG	150	1.2732926E-11	18.5	5
CTGCATC	2535	0.0	18.317553	13
CTAACCG	75	2.0681208E-4	17.266666	21
CATCAGA	2740	0.0	16.94708	16
ATCAGAA	2730	0.0	16.873627	17
GGCCATC	2770	0.0	16.49639	26
ATCAAGC	2730	0.0	16.467033	30
AGGCCAT	2780	0.0	16.370504	25
AGAGGCC	2880	0.0	16.123264	23
AAGAGGC	2860	0.0	16.106644	22
>>END_MODULE