FastQCFastQC Report
Fri 10 Feb 2017
ERR1630286.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630286.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2342307
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64160.2739179791547393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53970.23041386120606738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45400.19382600145924508No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26220.11194091978549353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC806.966475E-720.812528
GGTATCA21650.018.7136271
ATTAGAC1603.45608E-1117.343753
TCTATCG1107.8161065E-716.81818229
ACCGTCG3100.016.1129048
TATCGCC1059.351303E-615.85714231
TCCGCGA957.064678E-515.57894826
TACCGTC3400.015.2352937
ACGAACG2201.8189894E-1215.13636415
CGGTCGA1001.09413355E-414.820
ATACCGT3650.014.698636
TCTTATA15850.014.12302837
GTGTTAT1854.9058144E-914.01
CTAGCAC3050.013.9508193
CGGGTAA2700.013.70370324
CCGTCGT3550.013.5492969
CGACGGT2601.8189894E-1213.5192317
GACGGAC3900.013.2820527
CGTCGTA3650.013.17808210
CTATCGC1551.8897026E-613.12903230
Produced by FastQC (version 0.11.5)