##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630286.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2342307
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.522013980234018	31.0	31.0	34.0	30.0	34.0
2	31.744088200223114	31.0	31.0	34.0	30.0	34.0
3	31.864066068196866	33.0	31.0	34.0	30.0	34.0
4	35.57093369912654	37.0	35.0	37.0	33.0	37.0
5	35.457885751099234	37.0	35.0	37.0	33.0	37.0
6	35.519557000854284	37.0	35.0	37.0	33.0	37.0
7	35.50402146260076	37.0	35.0	37.0	33.0	37.0
8	35.50537397531579	37.0	35.0	37.0	33.0	37.0
9	37.198281864845214	39.0	37.0	39.0	34.0	39.0
10	37.03398956669643	39.0	37.0	39.0	33.0	39.0
11	37.128001581346936	39.0	37.0	39.0	33.0	39.0
12	37.02992605153808	39.0	37.0	39.0	33.0	39.0
13	37.056162578176135	39.0	37.0	39.0	33.0	39.0
14	38.21041135939909	40.0	38.0	41.0	33.0	41.0
15	38.206558747422946	40.0	38.0	41.0	33.0	41.0
16	38.156750161272626	40.0	38.0	41.0	33.0	41.0
17	38.12249760599273	40.0	37.0	41.0	33.0	41.0
18	38.069466982765285	40.0	37.0	41.0	33.0	41.0
19	38.10263300242026	40.0	37.0	41.0	33.0	41.0
20	38.07348353567658	40.0	37.0	41.0	33.0	41.0
21	38.01680010348772	40.0	37.0	41.0	33.0	41.0
22	37.983255824279226	40.0	37.0	41.0	33.0	41.0
23	37.91771872773296	40.0	37.0	41.0	33.0	41.0
24	37.87789516916442	40.0	37.0	41.0	33.0	41.0
25	37.864627907443385	40.0	37.0	41.0	33.0	41.0
26	37.67750640714475	40.0	37.0	41.0	32.0	41.0
27	37.56075569940234	39.0	37.0	41.0	32.0	41.0
28	37.48318687516197	39.0	37.0	41.0	32.0	41.0
29	37.420916216362755	39.0	37.0	41.0	32.0	41.0
30	37.35395488294233	39.0	36.0	41.0	32.0	41.0
31	37.2949007111365	39.0	36.0	40.0	31.0	41.0
32	37.19957119199149	39.0	36.0	40.0	31.0	41.0
33	37.10020548117732	39.0	36.0	40.0	31.0	41.0
34	37.04098736843633	39.0	36.0	40.0	31.0	41.0
35	36.914659777731956	39.0	36.0	40.0	30.0	41.0
36	36.82474884803742	39.0	36.0	40.0	30.0	41.0
37	36.761833098735565	39.0	35.0	40.0	30.0	41.0
38	36.65451070248264	39.0	35.0	40.0	30.0	41.0
39	36.580607922018764	39.0	35.0	40.0	30.0	41.0
40	36.47389133875278	39.0	35.0	40.0	30.0	41.0
41	36.363012192680124	39.0	35.0	40.0	30.0	41.0
42	36.318394642546856	39.0	35.0	40.0	30.0	41.0
43	35.07640117200692	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	5.0
11	3.0
12	1.0
13	7.0
14	4.0
15	9.0
16	26.0
17	52.0
18	115.0
19	267.0
20	521.0
21	967.0
22	1754.0
23	3101.0
24	5231.0
25	7982.0
26	12177.0
27	17118.0
28	24566.0
29	33325.0
30	43724.0
31	57205.0
32	72493.0
33	94020.0
34	122402.0
35	163240.0
36	228984.0
37	349278.0
38	618279.0
39	485445.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.632288167178764	19.847569084667384	12.869406102615924	25.650736645537926
2	18.063644091060652	21.339943909999842	35.36163278340542	25.234779215534086
3	19.32043920801159	23.69988221014581	30.19437674053828	26.78530184130432
4	14.417537923081817	16.21034305067611	35.27812536956086	34.09399365668121
5	14.218161837880347	37.02200437431985	34.56737310694115	14.192460680858657
6	33.91485403066293	36.17309771947059	15.97980111061445	13.932247139252027
7	28.944924811307825	30.96716186221533	21.82664356124112	18.261269765235728
8	26.741114636125836	34.99186912731764	19.838347407064916	18.428668829491606
9	26.574910974522126	14.597403329281772	19.761030471240534	39.06665522495557
10	16.428077105178783	27.2321262755053	33.366975379401595	22.972821239914325
11	35.714746188266524	22.251310353425062	21.96753030238991	20.066413155918504
12	21.08557930279848	25.786115995896353	30.20308610271839	22.925218598586778
13	29.564655700555054	20.03635731780676	25.488973050927992	24.910013930710193
14	22.35612155024939	20.629575883946895	25.623925471767787	31.39037709403592
15	24.636650959929675	28.317637269580803	22.45222338489361	24.59348838559591
16	24.630631253716956	26.798579349333796	24.291606522970728	24.279182873978517
17	23.332893595929143	27.094526891649984	25.815574132682013	23.757005379738864
18	23.41187555687619	25.725193153587465	26.880549816911277	23.982381472625068
19	24.149310914410453	25.915176789379018	26.799048971804297	23.136463324406236
20	24.198279730197623	25.55527520517165	26.598819027565558	23.647626037065166
21	24.474844672367883	25.743551122888675	26.062979788729656	23.718624416013785
22	24.41302527806987	25.525347445915504	26.23639855919826	23.82522871681637
23	23.693520960318182	25.77343618919296	26.49409321664496	24.0389496338439
24	23.979094115331595	26.11617520675129	26.187515129314814	23.717215548602297
25	24.320893887948934	25.276575615408227	26.656369126677248	23.746161369965595
26	24.016535834115682	26.04129176918312	26.383817321982132	23.55835507471907
27	24.151872491522248	25.608470623193284	26.095981440519967	24.143675444764497
28	23.672558720953315	25.800503520674273	26.766559635436344	23.760378122936064
29	23.57047987304824	26.04355449563187	26.7147730848262	23.671192546493693
30	23.385534005576552	26.48043147204871	26.43308498843234	23.700949533942392
31	24.067938148159058	25.92764313132309	26.343259017712022	23.661159702805822
32	23.420029910682075	25.993048733577623	26.402431448994516	24.184489906745785
33	23.408161270064088	25.743764587647988	26.812241093930044	24.03583304835788
34	24.17044392558277	25.441028865985544	26.405761499239848	23.982765709191835
35	24.033527628957263	25.57299278019491	26.86564997671099	23.52782961413683
36	23.65889697635707	25.804303193390105	26.438549686270846	24.098250143981982
37	23.95544221999934	25.116220888209785	26.77620824255744	24.152128649233426
38	23.454312351028282	25.359442634974833	27.154723953777193	24.031521060219692
39	23.54255868252966	25.093252080107348	27.243055671182297	24.121133566180696
40	23.387625960217854	24.92354759645085	27.775308702061686	23.91351774126961
41	22.672305551748767	25.291005833138016	28.03223488637484	24.004453728738376
42	23.275001953202548	25.121642893096425	27.727492595974823	23.875862557726208
43	22.940545368305692	24.64753766265481	27.995518947772428	24.416398021267067
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	388.0
1	379.0
2	370.0
3	731.0
4	1092.0
5	1092.0
6	1509.0
7	1926.0
8	1809.5
9	1693.0
10	2456.5
11	3220.0
12	3220.0
13	5372.0
14	7524.0
15	11561.5
16	15599.0
17	15601.5
18	15604.0
19	15604.0
20	18105.0
21	20606.0
22	21439.0
23	22272.0
24	26269.5
25	30267.0
26	30267.0
27	35064.0
28	39861.0
29	48957.0
30	58053.0
31	64209.0
32	70365.0
33	70365.0
34	79684.0
35	89003.0
36	98143.0
37	107283.0
38	119029.0
39	130775.0
40	130775.0
41	141102.0
42	151429.0
43	152475.5
44	153522.0
45	159892.5
46	166263.0
47	166263.0
48	170484.5
49	174706.0
50	176045.5
51	177385.0
52	178276.0
53	179167.0
54	179167.0
55	165928.0
56	152689.0
57	143743.0
58	134797.0
59	127305.0
60	119813.0
61	119813.0
62	109546.5
63	99280.0
64	86049.5
65	72819.0
66	62606.5
67	52394.0
68	52394.0
69	43696.0
70	34998.0
71	29684.0
72	24370.0
73	19460.5
74	14551.0
75	14551.0
76	11065.0
77	7579.0
78	6046.0
79	4513.0
80	3519.0
81	2525.0
82	2525.0
83	1898.5
84	1272.0
85	1093.5
86	915.0
87	821.0
88	727.0
89	727.0
90	641.5
91	556.0
92	326.5
93	97.0
94	66.0
95	35.0
96	35.0
97	19.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2342307.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.14907265369404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.67431913889178	42.07090930061367
2	10.74530271289737	11.207151437216414
3	3.3306235229279078	5.210667842378096
4	1.4889317489804472	3.105856398158923
5	0.8196662561036565	2.1372417570665485
6	0.5355500226899296	1.675706222576678
7	0.3885125547864578	1.4182398612501892
8	0.2713769462606996	1.1321644869669476
9	0.20301526731192118	0.9528352132372646
>10	1.2801127540842034	12.814240314546632
>50	0.15445172899236104	5.623547314815969
>100	0.10198288496867183	9.623431667935881
>500	0.005333866290580407	1.906568049121072
>1k	6.564758511483485E-4	0.6159233706147573
>5k	1.6411896278708713E-4	0.5055167635011553
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6416	0.2739179791547393	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5397	0.23041386120606738	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4540	0.19382600145924508	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2622	0.11194091978549353	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	4.269295186326984E-5	0.0	0.0	4.269295186326984E-5
4	0.0	4.269295186326984E-5	0.0	0.0	4.269295186326984E-5
5	0.0	4.269295186326984E-5	0.0	0.0	4.269295186326984E-5
6	0.0	4.269295186326984E-5	0.0	0.0	8.538590372653968E-5
7	0.0	4.269295186326984E-5	0.0	0.0	8.538590372653968E-5
8	0.0	4.269295186326984E-5	0.0	0.0	8.538590372653968E-5
9	0.0	4.269295186326984E-5	0.0	0.0	8.538590372653968E-5
10	0.0	4.269295186326984E-5	0.0	0.0	8.538590372653968E-5
11	0.0	8.538590372653968E-5	0.0	0.0	8.538590372653968E-5
12	0.0	1.2807885558980953E-4	0.0	4.269295186326984E-5	8.538590372653968E-5
13	0.0	1.2807885558980953E-4	0.0	4.269295186326984E-5	8.538590372653968E-5
14	0.0	1.2807885558980953E-4	0.0	4.269295186326984E-5	8.538590372653968E-5
15	0.0	1.2807885558980953E-4	0.0	4.269295186326984E-5	8.538590372653968E-5
16	0.0	1.2807885558980953E-4	0.0	2.988506630428889E-4	8.538590372653968E-5
17	0.0	1.2807885558980953E-4	0.0	5.123154223592381E-4	8.538590372653968E-5
18	0.0	1.2807885558980953E-4	0.0	5.977013260857778E-4	8.538590372653968E-5
19	0.0	1.2807885558980953E-4	0.0	7.257801816755873E-4	8.538590372653968E-5
20	0.0	1.2807885558980953E-4	0.0	9.819378928552064E-4	8.538590372653968E-5
21	0.0	1.2807885558980953E-4	0.0	0.0013234815077613651	1.2807885558980953E-4
22	0.0	1.2807885558980953E-4	0.0	0.0015369462670777143	1.2807885558980953E-4
23	0.0	1.2807885558980953E-4	0.0	0.0020492616894369525	1.2807885558980953E-4
24	0.0	1.2807885558980953E-4	0.0	0.0032446643416085084	1.2807885558980953E-4
25	0.0	1.2807885558980953E-4	0.0	0.004098523378873905	1.2807885558980953E-4
26	0.0	1.2807885558980953E-4	0.0	0.005848934405267969	1.7077180745307937E-4
27	0.0	1.2807885558980953E-4	0.0	0.014045981163015779	2.5615771117961907E-4
28	0.0	1.7077180745307937E-4	0.0	0.03838096372507959	2.5615771117961907E-4
29	0.0	1.7077180745307937E-4	0.0	0.07637769088338975	2.5615771117961907E-4
30	0.0	1.7077180745307937E-4	0.0	0.1305550467978792	2.5615771117961907E-4
31	0.0	1.7077180745307937E-4	0.0	0.31609861559564995	2.5615771117961907E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAC	80	6.966475E-7	20.8125	28
GGTATCA	2165	0.0	18.713627	1
ATTAGAC	160	3.45608E-11	17.34375	3
TCTATCG	110	7.8161065E-7	16.818182	29
ACCGTCG	310	0.0	16.112904	8
TATCGCC	105	9.351303E-6	15.857142	31
TCCGCGA	95	7.064678E-5	15.578948	26
TACCGTC	340	0.0	15.235293	7
ACGAACG	220	1.8189894E-12	15.136364	15
CGGTCGA	100	1.09413355E-4	14.8	20
ATACCGT	365	0.0	14.69863	6
TCTTATA	1585	0.0	14.123028	37
GTGTTAT	185	4.9058144E-9	14.0	1
CTAGCAC	305	0.0	13.950819	3
CGGGTAA	270	0.0	13.703703	24
CCGTCGT	355	0.0	13.549296	9
CGACGGT	260	1.8189894E-12	13.519231	7
GACGGAC	390	0.0	13.282052	7
CGTCGTA	365	0.0	13.178082	10
CTATCGC	155	1.8897026E-6	13.129032	30
>>END_MODULE