FastQCFastQC Report
Fri 10 Feb 2017
ERR1630285.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630285.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907230
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG34300.17984196976767353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32020.16788745982393313No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25970.13616606282409568No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG24490.12840611777289576No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT23920.12541749028696067No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA22390.11739538492997698No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC21780.11419702919941487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21000.11010732842918788No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC19970.10470682613004201No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGA400.00193165223.12532
GGTATCA13050.021.4061321
AACCGTA500.00703654318.4999987
TATACTG2250.018.0888885
CAAGACG4800.017.7291664
AAGACGG4200.017.6190475
TACCGTG650.0015804517.0769237
TTTCGGA3700.016.530
CGGACCA4400.016.3977289
GCGCAAG5300.016.0566041
GACGGAC4650.015.9139797
TCTAGCG2550.015.23529428
ACGGACC4750.015.1894738
CGGCCTT6100.014.86065624
CGAACGA2750.014.79999916
TCGCCAT5800.014.67241413
CGCAAGA5350.014.5233642
TTCCTCG6850.014.313868519
ACGAACG3000.014.18333315
GTATCAA20000.014.15252
Produced by FastQC (version 0.11.5)